Seasonal variations in the occurrence of<i>Golovinomyces orontii</i>and<i>Podosphaera xanthii</i>, causal agents of cucurbit powdery mildew in Northern Italy

Pirondi, A.; Pérez-Garcı́a, Alejandro; Battistini, G.; Muzzi, Enrico; Brunelli, A.

doi:10.1111/aab.12225

Cited by 17 publications

(24 citation statements)

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“…Moreover, multiplex PCR produced a species‐specific amplicon of 391 bp for all isolates that, according to Pirondi et al . (), confirmed the identity of G. orontii .…”

Section: Resultssupporting

confidence: 68%

“…This was also suggested by the Hudson's K ST test and F ST and N m indices, which indicated a low differentiation between the two populations. This could be explained by the fact that the spread of powdery mildews is caused by wind action that could efficiently transport conidia even to long distances and thus affect the composition of powdery mildew populations (Pirondi et al ., ). This has already been hypothesized for Czech G. orontii populations by Lebeda et al .…”

Section: Discussionmentioning

confidence: 97%

“…() and for the north of Italy by Pirondi et al . (), and also affects population genetics of cucurbit downy mildew, Pseudoperonospora cubensis (Lebeda & Cohen, ; Kitner et al ., ). The majority of the borders of the Czech Republic are natural mountain barriers, except for the southeastern region, which is relatively open to air currents from southern Europe, thus representing the main potential source of genetic material at the beginning of the cucurbit growing season (May–June) (Kitner et al ., ).…”

Section: Discussionmentioning

confidence: 99%

“…In Italy, G. orontii is responsible for the early powdery mildew infections on cucurbit cultivation in late spring and it remains the dominant species until the end of spring/mid-summer. Subsequently, Podosphaera xanthii (Braun & Takamatsu, 2000) progressively appears and becomes the main powdery mildew species infecting cucurbits until the end of the growing season (Pirondi et al, 2015b). Because powdery mildews are obligate parasites, they must be able to survive during seasons when susceptible host tissue is unavailable for infection.…”

Section: Introductionmentioning

confidence: 99%

“…Isolates, location, dates of collection and host plants are given in Table 1. Species identification was performed both by morphology of dry conidia (presence of fibrosin bodies and the position of the germ tube) in 3% KOH solution (Lebeda, 1983) and by multiplex PCR using the species-specific primers for the internal transcribed spacer (ITS) regions of rDNA described by Pirondi et al (2015b).…”

Section: Introductionmentioning

confidence: 99%

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Genetic structure and phylogeny of Italian and Czech populations of the cucurbit powdery mildew fungus Golovinomyces orontii inferred by multilocus sequence typing

et al. 2015

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Cucurbit powdery mildew caused by Golovinomyces orontii is a serious disease that affects cucurbit crops in temperate areas. In northern Italy, the species is responsible for the early infections at the beginning of the growing season. However, chasmothecia have never been recorded in Italy and the impact of either asexual or sexual reproduction of G. orontii remains to be determined. To investigate and compare the genetic structure of Italian populations with those from the Czech Republic, where chasmothecia are sporadically recorded, seven housekeeping gene fragments (tef-1a, csI, ITS, H3, tub2, IGS and mtLSU) were amplified and used in a multilocus sequence typing (MLST) approach. The four-gamete test was performed to detect recombination between and within populations. Phylogeny was inferred using both Bayesian and minimum evolution analyses. Results from MLST revealed the presence of 141 single nucleotide polymorphisms and the existence of 13 different haplotypes. Phylogenetic analysis revealed the existence of two main distinct phylogenetic groups that were also highly genetically different, confirming that G. orontii is a species complex. Recombination was detected within both the phylogenetic groups and the geographical populations, indicating that sexual reproduction could have occurred. However, considering the lower haplotype diversity and the high frequency of ST3 (17 isolates) and ST7 (eight isolates) haplotypes, the Italian population could be predominantly clonal, while sexual reproduction may occasionally occur due to the introduction of genotypes similar to those from the Czech Republic.

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“…Moreover, multiplex PCR produced a species‐specific amplicon of 391 bp for all isolates that, according to Pirondi et al . (), confirmed the identity of G. orontii .…”

Section: Resultssupporting

confidence: 68%

Section: Discussionmentioning

confidence: 97%