Seasonal Variation on Growth and Saikosaponins Content of Bupleurum falcatum L.

Pharmacologically Active Saikosaponin inBupleurum falcatumDetected by Competitive ELISA and Eastern Blotting Using Monoclonal Antibodies

“…On the other hand, no conspicuous color development was observed in phloem and xylem inside the cork. This was similar to the results obtained by Shon et al (1997a) and Minami et al (1995), wherein total saikosaponin content was higher in outer tissues than in the inner tissues of the roots. Saikosaponin was also detected in the leaf tissues by immunohistochemical analysis (Fig.…”

“…It has been reported that secondary metabolites accumulated in cork layer or xylem of Bupleurum falcatum (Minami et al, 1995). The cork tissue was found to contain higher concentration of SSa than phloem and xylem by using ELISA, indicating a good agreement with the above immunohistochemical analysis.…”

“…Furthermore, both analyses showed a higher concentration of SSa in the Jeongsun strain. Shon et al (1998) and Minami et al (1995) found that the clear polymorphism of B. falcatum in both morphological property and concentration of SSa depended on different habitats, but the concentrations of SSc and d were within the standard deviation. Genetic variation was also investigated in this species.…”

“…The cork tissue was found to contain higher concentration of SSa than phloem and xylem by using ELISA, indicating a good agreement with the above immunohistochemical analysis. With regard to the distribution of saikosaponins in the main root, saikosaponins accumulated most in the outer tissues of the phloem layer, especially in the pericycle and its neighboring tissues (Minami et al, 1995;Shon et al, 1997a).…”

Haploid Plantlet Production through Somatic Embryogenesis in Anther-Derived Callus ofBupleurum falcatum

“…It is indispensable to clarify the characteristics of dry matter production, saikosaponin production, and solar energy utilization (Shon et al, 1998;Minami et al, 1995), for genetic improvement of B. falcatum and also to fi nd a way of shortening the breeding intervals for obtaining high yield of high quality roots. Recently, anther culture is widely used to obtain haploid plants and for shortening the breeding intervals.…”