Determination of saikosaponins by micellar electrokinetic capillary chromatography

Hsieh, You‐Zung; Huang, Hsi Ya

doi:10.1016/s0021-9673(96)00768-6

Cited by 24 publications

(10 citation statements)

References 17 publications

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“…1.3-1.5, SSd: 1.5-4.8 mg/g) [23], LC-MS/MS (SSa: 2.1-3.6, SSc: 1.1-2.1, SSd: 0.9-1.1) [25], LC-MS (SSa: 5.9-10.1, SSc: 1.3-3.4 mg/g) [26], or MEKC-UV (SSa: 1.0, SSb 2 : 0.8, SSd: 12.1 mg/g) [21]. Variations in the saikosaponin contents of Bupleuri Radix might be due to environmental or storage conditions and/or seasonal effects.…”

Section: Applicationmentioning

confidence: 99%

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Analysis of saikosaponins in Bupleuri Radix and Caihu‐shugan‐san using reversed‐phase HPLC with pulsed amperometric detection

Kwon

Sim

Lee

et al. 2011

J of Separation Science

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A simple and sensitive reversed-phase (RP) HPLC coupled with pulsed amperometric detection (PAD) method was developed to determine the saikosaponin content in Bupleuri Radix or Caihu-shugan-san. Four saikosaponins in Bupleuri Radix and Caihu-shugan-san were extracted with a 6:4 solution of 10 mM sodium phosphate buffer (pH 8)/100% ethanol. Pulsed amperometric detection of carbohydrates in four major saikosaponins was highly sensitive when used with a water-acetonitrile gradient on an alkaline RP column with a post-column delivery system. The limits of detection (S/N=3) and of quantification (S/N=10) of saikosaponins were 0.01-0.02 and 0.03-0.05 μg/mL, respectively. The intra- and inter-day precision (RSDs) were each <9.7% and the average recoveries were 95.0-97.6% in Bupleuri Radix. This method can be used to analyze saikosaponins in Bupleuri Radix and Caihu-shugan-san.

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Section: Applicationmentioning

confidence: 99%

“…The HPLC-UV and MEKC-UV methods display low sensitivities due to the poor chromophores and short wavelengths (r220 nm) of SSa, SSc, and SSd [20,21]. The HPLC-ELSD method has a slightly increased sensitivity compared to HPLC-UV [22].…”

Section: Introductionmentioning

confidence: 99%

Analysis of saikosaponins in Bupleuri Radix and Caihu‐shugan‐san using reversed‐phase HPLC with pulsed amperometric detection

Kwon

Sim

Lee

et al. 2011

J of Separation Science

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“…These saikosaponins have been determined previously in various species of Chaihu through the use of HPLC with UV detection at 210 nm [4] and the use of micellar electrokinetic capillary chromatography [5]. However, for quantitative analysis of saikosaponins in medicinal preparations containing other plants, interference is common and it is difficult to obtain good results.…”

Section: Introductionmentioning

confidence: 99%

Quality control of Chinese medicinal preparations

Liau

Hsiao

Lee

et al. 2007

Journal of Pharmaceutical and Biomedical Analysis

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“…Capillary electrophoresis (CE) has become one of the most useful techniques for the monitoring of drugs [4,5,6,7]. However, the shortcoming of CE is high detection limits due to the limited optical path length for on-line UV detection and the small amount of sample loaded into the capillary.…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis with field-enhanced stacking for rapid and sensitive determination of strychnine and brucine

et al. 2003

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A new capillary electrophoresis procedure with field-enhanced stacking concentration for the analysis of strychnine and brucine is established. After optimization of the separation and concentration conditions, the two alkaloids can be separated within 5 min and quantified with high sensitivity (The detection limits were 1.0 ng mL(-1) for strychnine and 1.4 ng mL(-1) for brucine). The method was useful for qualitative and quantitative analysis of strychnine and brucine in Strychnos nux-vomica L with recovery of 105.1% for strychnine and 98.4% for brucine.

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Determination of saikosaponins by micellar electrokinetic capillary chromatography

Cited by 24 publications

References 17 publications

Analysis of saikosaponins in Bupleuri Radix and Caihu‐shugan‐san using reversed‐phase HPLC with pulsed amperometric detection

Analysis of saikosaponins in Bupleuri Radix and Caihu‐shugan‐san using reversed‐phase HPLC with pulsed amperometric detection

Quality control of Chinese medicinal preparations

Capillary electrophoresis with field-enhanced stacking for rapid and sensitive determination of strychnine and brucine

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