Seasonal variation of Brazilian red propolis: Antibacterial activity, synergistic effect and phytochemical screening

Regueira-Neto, Marcos da Silveira; Tintino, Saulo Relison; Silva, Ana Raquel Pereira da; Costa, Maria do Socorro; Boligon, Aline Augusti; Matias, Edinardo Fágner Ferreira; Balbino, Valdir Queiroz; Menezes, Irwin Rose Alencar de; Coutinho, Henrique Douglas Melo

doi:10.1016/j.fct.2017.03.052

Cited by 93 publications

(80 citation statements)

References 53 publications

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“…However, when we combined the same red propolis extract with imipenem, we only had synergistic effect against P. aeruginosa strains. In a previous work, we found similar results when we combined red propolis collected in the state of Pernambuco during the dry season . Here we have the first report of the synergistic behavior between Dalbergia ecastaphyllum resin and the antibacterial drugs gentamicin and imipenem.…”

Section: Discussionsupporting

confidence: 82%

“…Drug resistance is a major concern in hospitals over the world, accordingly, many studies have been carried out testing antibacterial drugs association with natural products. The synergistic behavior between antimicrobial drugs and propolis from different types and geographical regions have been performed against Staphylococcus aureus , Pseudomonas aeruginosa , Salmonella and Candida strains and the researches successfully obtained synergistic effect with those association . In our present study, when we combined the hydroethanolic extract of red propolis with gentamicin, we could observe synergistic effect against all strains used in this study.…”

Section: Discussionsupporting

confidence: 52%

“…This phenolic acid, which is not present in our sample collected in Alagoas, along with it caffeic acid, quercetin and luteolin were previously reported in other samples of red propolis from Alagoas and Pernambuco. [13,15,17,25,30] Chlorogenic acid, ellagic acid and apigenin were also reported in red propolis from Pernambuco. [17] Quercetin and luteolin were also found in D. ecastaphyllum resin from previous studies using samples from Alagoas.…”

Section: Discussionmentioning

confidence: 99%

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Comparative Analysis of the Antibacterial Activity and HPLC Phytochemical Screening of the Brazilian Red Propolis and the Resin of Dalbergia ecastaphyllum

Regueira-Neto¹,

Tintino

Silva

et al. 2019

Chemistry & Biodiversity

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The aim of this study was to investigate the antibacterial activity of red propolis and resin and their association with standard antibiotics to evaluate possible differences of activity. We also submitted red propolis and the resin to a HPLC analysis to confirm the botanical origin. The extracts were tested against P. aeruginosa and S. aureus alone and in association with gentamicin and imipenem. The HPLC analysis identified seven compounds with six of them present in both substances. The lowest MIC values obtained in this study were observed against S. aureus. In general, MIC values showed to be lower for red propolis against all species tested in comparison to resin. Despite the synergistic behavior to be similar for both substances, we observed that inhibitory concentrations of drugs were lower when associated with red propolis in comparison to resin.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 99%

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Comparative Analysis of the Antibacterial Activity and HPLC Phytochemical Screening of the Brazilian Red Propolis and the Resin of Dalbergia ecastaphyllum

Regueira-Neto¹,

Tintino

Silva

et al. 2019

Chemistry & Biodiversity

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“…According to literature, the propolis mechanisms of action are inhibition of nucleoside synthesis, inhibition mechanism of DNA‐gyrase, inhibition of cytoplasmic membrane, inhibition of energy metabolism and inhibition of biofilm formation . The mechanism, which silver acts is through the release of positively charged Ag ions that bind the wall of the bacterium causing cell wall disruption, deactivation of enzymes and causing changes in membrane permeability .…”

Section: Resultsmentioning

confidence: 99%

“…54 According to literature, the propolis mechanisms of action are inhibition of nucleoside synthesis, inhibition mechanism of DNA-gyrase, inhibition of cytoplasmic membrane, inhibition of energy metabolism and inhibition of biofilm formation. 55 The mechanism, which silver acts is through the release of positively charged Ag ions that bind the wall of the bacterium causing cell wall disruption, deactivation of enzymes and causing changes in membrane permeability. 56 It is believed that the differential of the nanoparticles is given by their greater surface area that allows a stronger interaction in the therapeutic sites, besides its smaller size to facilitate penetration through the biological membranes.…”

Section: Antibacterial Evaluationmentioning

confidence: 99%

Biogenic synthesis of silver nanoparticles using Brazilian propolis

Barbosa

Souza

Alvino

et al. 2019

Biotechnology Progress

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Biological methods have been used to synthesize silver nanoparticles through materials such as bacteria, fungi, plants, and propolis due to their reducing properties, stabilizer role and environmentally friendly characteristic. Considering the antimicrobial activity of propolis as well as the broad‐spectrum antibacterial effects of silver nanoparticles, this study aim to describe the use of Brazilian propolis to synthesize silver nanoparticles (AgNP‐P) and investigate its antimicrobial activity. The synthesis was optimized by factorial design, choosing the best conditions for smaller size particles. AgNP‐P demonstrated a maximum absorbance at 412 nm in ultraviolet‐visible spectra, which indicated a spherical format and its formation. Dynamic light scattering demonstrated a hydrodynamic size of 109 nm and polydispersity index less than 0.3, showing a good size distribution and stability. After its purification via centrifugation, microscopy analysis corroborates the format and showed the presence of propolis around silver nanoparticle. X‐ray diffraction peaks were attributed to the main planes of the metallic silver crystalline structure; meanwhile infrared spectroscopy demonstrated the main groups responsible for silver reduction, represented by ∼22% of AgNP‐P indicates by thermal analysis. Our product revealed an important antimicrobial activity indicating a synergism between propolis and silver nanoparticles as expected and promising to be an effective antimicrobial product to be used in infections.

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Green and Red Brazilian Propolis: Antimicrobial Potential and Anti‐Virulence against ATCC and Clinically Isolated Multidrug‐Resistant Bacteria

Silva

Braun

et al. 2021

Chemistry & Biodiversity

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Brazilian green and red propolis stand out as commercial products for different medical applications. In this article, we report the antimicrobial activities of the hydroalcoholic extracts of green (EGP) and red (ERP) propolis, as well as guttiferone E plus xanthochymol (8) and oblongifolin B (9) from red propolis, against multidrug‐resistant bacteria (MDRB). We undertook the minimal inhibitory (MIC) and bactericidal (MBC) concentrations, inhibition of biofilm formation (MICB50), catalase, coagulase, DNase, lipase, and hemolysin assays, along with molecular docking simulations. ERP was more effective by displaying MIC and MBC values <100 μg mL−1. Compounds 8 and 9 displayed the lowest MIC values (0.98 to 31.25 μg mL−1) against all tested Gram‐positive MDRB. They also inhibited the biofilm formation of S. aureus (ATCC 43300 and clinical isolate) and S. epidermidis (ATCC 14990 and clinical isolate), with MICB50 values between 1.56 and 6.25 μg mL−1. The molecular docking results indicated that 8 and 9 might interact with the catalase's amino acids. Compounds 8 and 9 have great antimicrobial potential.

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Seasonal variation of Brazilian red propolis: Antibacterial activity, synergistic effect and phytochemical screening

Cited by 93 publications

References 53 publications

Comparative Analysis of the Antibacterial Activity and HPLC Phytochemical Screening of the Brazilian Red Propolis and the Resin of Dalbergia ecastaphyllum

Comparative Analysis of the Antibacterial Activity and HPLC Phytochemical Screening of the Brazilian Red Propolis and the Resin of Dalbergia ecastaphyllum

Biogenic synthesis of silver nanoparticles using Brazilian propolis

Green and Red Brazilian Propolis: Antimicrobial Potential and Anti‐Virulence against ATCC and Clinically Isolated Multidrug‐Resistant Bacteria

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