Seasonal variation in the phenol content of Eugenia uniflora L. leaves

Santos, Rosa; Oliveira, Marilene Silva; Ferri, Pedro Henrique; Santos, Suzana da Costa

doi:10.1590/s1516-05722011000100013

Cited by 6 publications

(6 citation statements)

References 19 publications

(2 reference statements)

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“…The total phenolic composition found for C. sinensis was similar to those found by other authors while for E. uniflora higher values were reported. [ 38 39 ]…”

Section: Discussionmentioning

confidence: 99%

Synergisms in alpha-glucosidase inhibition and antioxidant activity of Camellia sinensis L. Kuntze and Eugenia uniflora L. Ethanolic Extracts

Vinholes

Vizzotto

2017

Phcog Res

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Background:Camellia sinensis, the most consumed and popular beverages worldwide, and Eugenia uniflora, a Brazilian native species, have been already confirmed to have beneficial effects in the treatment of diabetes mellitus. However, their potential acting together against an enzyme linked to this pathology has never been exploited.Objective:The aim of this study was to evaluate the inhibitory properties of individual and combined ethanolic extracts of the leaves of C. sinensis and E. uniflora over alpha-glucosidase, a key digestive enzyme used on the Type 2 diabetes mellitus (T2DM) control. In addition, their inhibitory activity against 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•) and peroxyl radicals was also assayed.Materials and Methods:Enzyme inhibition and antioxidant potential were assessed based on in vitro assays. Total phenolic compounds, carotenoids, and chlorophylls A and B were achieved using spectrophotometric methods.Results:E. uniflora was almost 40 times more active on alpha-glucosidase than C. sinensis and combined extracts showed a significant synergistic effect with an obtained IC50 value almost 5 times lower than the theoretical value. C. sinensis extract was twice more active than E. uniflora concerning DPPH•, in contrast, E. uniflora was almost 10 times more effective than C. sinensis on inhibition of peroxyl radicals with a significant synergistic effect for combined extracts. The extracts activities may be related with their phytochemicals, mainly phenolic compounds, and chlorophylls.Conclusion:Combined C. sinensis and E. uniflora ethanolic extracts showed synergistic effect against alpha-glucosidase and lipid peroxidation. These herbal combinations can be used to control postprandial hyperglycemia and can also provide antioxidant defenses to patients with T2DM.SUMMARY Alfa-glucosidase and antioxidant Interaction between Camellia sinensis L. Kuntze and Eugenia uniflora L. ethanolic extracts was investigated.Extracts showed synergistic effect over alpha-glucosidase and peroxyl radicals.Total phenolic, carotenoids and chlorophylls A and B can be responsible by the observed activities.Extracts could be used as alternative to control postprandial hyperglycemia.Extracts could increase antioxidant defenses to patients with T2DM. Abbreviations Used: T2DM: Type 2 diabetes mellitus; DPPH: 2,2-diphenyl-1-picrylhydrazyl radical; PNPG: 4-Nitrophenyl β-D-glucuronide; LOO: Lipid peroxidation; SEM: Standard error of the mean; CAE: Chlorogenic acid equivalent

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“…The phenolic compounds have the capacity to inhibit the lipid peroxidation and lipoxygenase. The antioxidant properties of phenolic compounds are due to the chemical structure and the oxidoreduction properties with performance in the sequestration or neutralization of free radicals without causing damages to cell structures (SANTOS et al, 2011;SCHNEI-DER et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF Brunfelsia uniflora LEAF EXTRACT

Thiesen

Silva²,

Kubiak³

et al. 2019

ArqVet

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Finding bioactive compounds with antimicrobial and antioxidant capacity from natural sources has been a challenge., mainly due to the increase in microbial resistance. This study aimed at prospecting the main classes of secondary metabolites and the antioxidant and antimicrobial activity of Brunfelsia uniflora leaf extract. The ethanolic extract was obtained by dynamic maceration with solvent, and the antioxidant activity was analyzed using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. The antimicrobial activity was evaluated against Escherichia coli, Staphylococcus aureus, and Candida albicans by broth microdilution method. The phytochemical analysis of B. uniflora leaf extract showed the presence of saponins, tannins and alkaloids. The extract presented minimum inhibitory concentration of 62.5 mg/mL for E. coli. The 1.49 mg/mL extract concentration inhibited 50% of free radicals in the DPPH solution at 60 µM. The antimicrobial and antioxidant activities of this plant extract were the basis of studies to develop applications in the pharmaceutical, cosmetics and food industries.

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“…In other plants, to the contrary, there can be stabilization in the synthesis of some metabolites 35 . However, other factors must also be evaluated so that the role of flavonoids can be understood in the adaptation of this plant in its surroundings.…”

Section: Resultsmentioning

confidence: 99%

Untitled

2014

IJPSR

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Thuja occidentalis L. is a plant widely used therapeutically. Having seen the necessity to standardize the vegetal drug and to evaluate the interference of certain environmental and seasonal factors on the synthesis of metabolites, notably flavonoids, this work considered anatomical and physical-chemical characterization assays for the aforesaid vegetal drug, in addition to phytochemical prospecting and evaluation of the flavonoid content for one year. Characterization demonstrated that a thick powder (484 µm), with loss by desiccation (6.9%), water determination (5.3%), ash content (4.5%) and volatile oils (0.7%) to be acceptable. The average content of total polysaccharides obtained for analysis was 233.2 mg/g. The anatomical evaluation pointed to structures such as spongy parenchyma, secretor channels, heteromorphic cells and ciclocitic stomata. Phytochemical prospecting showed the presence of the majority of the metabolites investigated in all the months of study. Alkaloids and coumarins were absent. The verified results for flavonoid content indicate that T. occidentalis presents a dynamic metabolism with maximum attainment of flavonoids in march/2012, with a suggestive relationship with the verified low pluviometric index. The characterization data correspond to the first parameters for quality that allow for standardization of the vegetal drug and future phytomedicines obtained from this plant.

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“…In studies with Eugenia uniflora L. production of tannins was also higher in the dry season, the author reports that this fact is probably in response to the combination of higher incidence of light and drought stress [25]. Seasonal variations in leaf tannins have already been reported for deciduous trees from temperate climates, such as Quercus robur [26] and Betula pubescens [27].…”

Section: Phenolic Compounds Content Of Climatological Periodsmentioning

confidence: 86%

Tree Crown Variation and Seasonal in the Phenolic Compounds Content of Stryphnodendron adstringens (Mart) Coville Leaves

Ribeiro¹,

Goulart²,

Mori³

et al. 2014

AJPS

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The aim of this study was to compare phenolic compounds contents between different positions within the crown and between collection periods, and to evaluate the tannin contents by the Folin-Ciocalteu method. The collections were performed in Lavras, in the southern region of the Brazilian state Minas Gerais. The experiment was conducted in a completely randomized design (factorial 3 × 4); the first factor being the position (base, middle, top); and the months, the second factor (February, May, September and December). We verified that the leaves of S. adstringens present higher total phenolic and tannin contents at the top (23.05% and 18.23%) and base (21.42% and 16.61%) of the crown, in May (25.79% and 20.85%) and September (25.57% and 20.50%), respectively. There was no difference in the residual phenol and flavonoid contents between the positions within the crown or in the months of collection. These facts suggest that within the crown and climatic changes may be one of the factors affecting phenol levels in Stryphnodendron adstringens.

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“…The drop in the concentration of GA and EA from April and July to October may be related to the low rainfall that is related to low humidity, corroborated by data from Santos et al 37 in which the months with lower rainfall had lower levels of phenols and tannins condensed in the bark of "barbatimão" species. In a study with E. uniflora leaves, Santos et al 38 concluded that in the dry season (May to October) there is an increase in the amount of phenols and flavonoids. In the rainy season (November to March), the hydrolysable tannins increase.…”

Section: Co-validation For Crude Ethanol Extract (Cee) and Tannin-ric...mentioning

confidence: 99%

Validation HPLC-DAD Method for Quantification of Gallic and Ellagic Acid from Eugenia punicifolia Leaves, Extracts and Fractions

Silva¹,

Oliveira²,

Martins³

et al. 2023

J. Braz. Chem. Soc.

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Eugenia punicifolia (Kunth) DC, known as “pedra ume-caá” or “pitanguinha do cerrado”, is popularly used to treat infectious diseases like inflammation, and diabetes. Therefore, this study aimed to develop and validate an analytical method by high-performance liquid chromatography with diode array detector (HPLC-DAD) for the seasonal quantification of gallic acid (GA) and ellagic acid (EA), and to co-validate the method for quantification from the ethanol extract and fractions of the E. punicifolia leaves. Chromatographic separations were performed with a mobile phase gradient of acetonitrile/methanol/water acidified with 0.2% formic acid, a flow of 0.8 mL min‑1, detection at 254 nm, and a C18 column (250 × 4.6 mm, 5 μm). Method validation parameters were determined following Brazilian legislation. The HPLC-DAD analytical method developed for the quantification of GA and EA was validated and demonstrated to be selective, linear, precise, accurate, robust, and without matrix effect. Accordingly, it is helpful for the analysis of these tannins in crude ethanol extracts and fractions from E. punicifoila leaves. Thus, our results may be of great help for quality control assessment and standardization of raw materials containing Eugenia punicifolia.

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Seasonal variation in the phenol content of Eugenia uniflora L. leaves

Cited by 6 publications

References 19 publications

Synergisms in alpha-glucosidase inhibition and antioxidant activity of Camellia sinensis L. Kuntze and Eugenia uniflora L. Ethanolic Extracts

ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF Brunfelsia uniflora LEAF EXTRACT

Untitled

Tree Crown Variation and Seasonal in the Phenolic Compounds Content of Stryphnodendron adstringens (Mart) Coville Leaves

Validation HPLC-DAD Method for Quantification of Gallic and Ellagic Acid from Eugenia punicifolia Leaves, Extracts and Fractions

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