Seasonal variation in glycoconjugates of the pedal glandular system of the rayed Mediterranean limpet, Patella caerulea (Gastropoda: Patellidae)

Petraccioli, Agnese; Maio, Nicola; Guarino, Fabio Maria; Scillitani, Giovanni

doi:10.1016/j.zool.2012.10.006

Cited by 13 publications

(11 citation statements)

References 35 publications

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“…Only for KLH 2-c, a functional unit of keyhole limpet hemocyanin, a positive binding to peanut agglutinin indicated mucin-type O- glycosylation [95]. Histochemical and lectin-histochemical studies on the pedal glandular system of the rayed Mediterranean limpet, Patella caerulea , revealed different expressions of Gal, GalNAc, α1-2- and α1-4-linked Fuc and carboxylated and/or sulphated structures, presumably in O- glycans, in the course of the annual cycle [96]. In some snail tissues, traces of sialic acids were identified but could not be assigned to specific glycans [97].…”

Section: Molluscamentioning

confidence: 99%

Mucin-Type O-Glycosylation in Invertebrates

Staudacher

2015

Molecules

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Abstract:O-Glycosylation is one of the most important posttranslational modifications of proteins. It takes part in protein conformation, protein sorting, developmental processes and the modulation of enzymatic activities. In vertebrates, the basics of the biosynthetic pathway of O-glycans are already well understood. However, the regulation of the processes and the molecular aspects of defects, especially in correlation with cancer or developmental abnormalities, are still under investigation. The knowledge of the correlating invertebrate systems and evolutionary aspects of these highly conserved biosynthetic events may help improve the understanding of the regulatory factors of this pathway. Invertebrates display a broad spectrum of glycosylation varieties, providing an enormous potential for glycan modifications which may be used for the design of new pharmaceutically active substances. Here, overviews of the present knowledge of invertebrate mucin-type O-glycan structures and the currently identified enzymes responsible for the biosynthesis of these oligosaccharides are presented, and the few data dealing with functional aspects of O-glycans are summarised.

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Section: Molluscamentioning

confidence: 99%

Mucin-Type O-Glycosylation in Invertebrates

Staudacher

2015

Molecules

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“…Furthermore, the sulphated and fucosylated glycoproteins (HID, AAA, UEA I and LTA reactivity) could play an important role in microbial interactions. 49 A morpho-functional interpretation of our results requires comparison with literature data, but this is difficult because of both the absence of such investigations and the different terminology used in reported studies. 10,51 Ultrastructural analysis of the O. vulgaris epidermis revealed the presence of three type of mantle secretory cells (type 1, 2, and 3) but no data concerning the type of secreted mucosubstances.…”

Section: Discussionmentioning

confidence: 90%

“…48 The presence of the sialic acid is an unusual finding in mollusks, where it is usually substituted by muramic acid. 45,49 Differently from the other monosaccharides, the backbone of this sugar can exhibit pronounced chemical diversity in structure and linkage so that all the known sialic acids constitute the sialome. 50 Thus, sialome analysis could be a useful tool for monitoring health conditions of paralarvae from hatching to settlement.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the skin mucus in the common octopus Octopus vulgaris (Cuvier) reared paralarvae

et al. 2017

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The Octopus vulgaris farming is impaired by the high mortality of the paralarvae during the first month of life. Several factors have been investigated in this regard, but no data exist on the body surface mucus, which represents the interface with the outside environment. This study included morphometric analysis and glycoconjugates characterization of skin mucus in reared Octopus vulgaris paralarvae during the first month of life. Four types of mucous cells were distinguished: mucous 1 (m1) and mucous 2 (m2) cells were scattered in the mantle epidermis, mucous 3 (m3) and mucous 4 (m4) in the epithelium surrounding the sucker. Except for the presence of fucosylated and neutral glycoconjugates in all mucous cells, each cell type expressed a characteristic glycopattern. m2 and m4 contained also suphate and acid non-sulphate glycans, m3 lacked sulphate glycoproteins. Lectin histochemistry showed that mantle mucous cells (m1, m2) expressed GlcNAc and lactosamine terminating glycans. m2 also contained GalNAc terminal or penultimate to sialic acid. m3 was distinguished by mannosylated glycans terminating with lactosamine and m4 by α2,6 sialoglycans. Glycoproteins terminating with lactosamine, Galβ1,3GalNAc, and α1,6-linked fucose were a common feature of paralarvae surface layer. Morphometry revealed a significant decrease of m1 and m2 abundance during the first month of life, afterwards the reared paralarvae died. Since the glycopattern did not change during the investigated period, the mantle mucous cells abundance could be related to the Octopus vulgaris paralarvae survival.

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“…Protocol details were given elsewhere. 34 All the reactives cited in this section were from Sigma-Aldrich (St. Louis, MO).…”

Section: Methodsmentioning

confidence: 99%

High-fat Diet Alters the Glycosylation Patterns of Duodenal Mucins in a Murine Model

Mastrodonato

Calamita

Mentino

et al. 2020

J Histochem Cytochem.

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High-fat diet (HFD) alters the glycosylation patterns of intestinal mucins leading to several health problems. We studied by histochemical and lectin-binding methods mucin alterations in the duodenum of mice fed a HFD for 25 weeks. Histochemical methods included periodic acid–Schiff, alcian blue pH 2.5, and high-iron diamine. Lectin-binding experiments were performed with SBA, PNA, WGA, MAA-II, SNA, ConA, UEA-I, LTA, and AAA. SBA, PNA, WGA, MAA-II, and SNA were tested also after desulfation and ConA after periodate-sodium borohydrate treatments (paradoxical ConA). Duodenal mucins are secreted by Brunner’s glands and goblet cells in the villi. Brunner’s glands of HFD mice showed increased secreting activity and a general reduction of glycosylated residuals, such as fucose and terminal α1,4-linked GlcNAc. Moreover, a general reduction of glycosylated residuals in the goblet cells of villi such as the fucosylated and sulfated ones was observed. Since the cited residuals are involved in cytoprotective and cytostatic functions, as well as in interactions with the intestinal microbiota and protection against parasites and inflammatory disorders, we conclude that HFD can predispose duodenum to several possible health disorders.

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Seasonal variation in glycoconjugates of the pedal glandular system of the rayed Mediterranean limpet, Patella caerulea (Gastropoda: Patellidae)

Cited by 13 publications

References 35 publications

Mucin-Type O-Glycosylation in Invertebrates

Mucin-Type O-Glycosylation in Invertebrates

Characterization of the skin mucus in the common octopus Octopus vulgaris (Cuvier) reared paralarvae

High-fat Diet Alters the Glycosylation Patterns of Duodenal Mucins in a Murine Model

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