High-fat Diet Alters the Glycosylation Patterns of Duodenal Mucins in a Murine Model

Mastrodonato, María; Calamita, Giuseppe; Mentino, Donatella; Scillitani, Giovanni

doi:10.1369/0022155420911930

Cited by 20 publications

(20 citation statements)

References 48 publications

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“…24 Previous studies have also shown that dietary fiber inclusion can induce changes in mucin glycosylation. [25][26][27] However, due to limitations of the techniques used, these studies could only distinguish changes in acidic, neutral, or fucosylated glycans and lacked detailed structural characterization of the gut mucin glycans.…”

Section: Introductionmentioning

confidence: 99%

Changes in dietary fiber intake in mice reveal associations between colonic mucin O-glycosylation and specific gut bacteria

et al. 2020

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The colonic mucus layer, comprised of highly O-glycosylated mucins, is vital to mediating host-gut microbiota interactions, yet the impact of dietary changes on colonic mucin O-glycosylation and its associations with the gut microbiota remains unexplored. Here, we used an array of omics techniques including glycomics to examine the effect of dietary fiber consumption on the gut microbiota, colonic mucin O-glycosylation and host physiology of high-fat diet-fed C57BL/6J mice. The high-fat diet group had significantly impaired glucose tolerance and altered liver proteome, gut microbiota composition, and short-chain fatty acid production compared to normal chow diet group. While dietary fiber inclusion did not reverse all high fat-induced modifications, it resulted in specific changes, including an increase in the relative abundance of bacterial families with known fiber digesters and a higher propionate concentration. Conversely, colonic mucin O-glycosylation remained similar between the normal chow and high-fat diet groups, while dietary fiber intervention resulted in major alterations in O-glycosylation. Correlation network analysis revealed previously undescribed associations between specific bacteria and mucin glycan structures. For example, the relative abundance of the bacterium Parabacteroides distasonis positively correlated with glycan structures containing one terminal fucose and correlated negatively with glycans containing two terminal fucose residues or with both an N-acetylneuraminic acid and a sulfate residue. This is the first comprehensive report of the impact of dietary fiber on the colonic mucin O-glycosylation and associations of these mucosal glycans with specific gut bacteria.

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Section: Introductionmentioning

confidence: 99%

Changes in dietary fiber intake in mice reveal associations between colonic mucin O-glycosylation and specific gut bacteria

et al. 2020

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“…So, it can considerably weaken the response of proliferation of GCs that are caused by the stimulation of the fibers. HFD not only contains less fibers but also can effectively reduce glycosylated residuals in the GCs, 35 elevate the enteral and circulating bile acid levels, such as deoxycholic acid, which are cytotoxic, 36 thereby significantly decreasing the number of GCs. Thus, it is feasible that exercise may ameliorate these undesirable physiological and biochemical processes by balancing energy metabolism.…”

Section: Discussionmentioning

confidence: 99%

Moderate Treadmill Exercise Modulates Gut Microbiota and Improves Intestinal Barrier in High-Fat-Diet-Induced Obese Mice via the AMPK/CDX2 Signaling Pathway

Wang¹,

Zhang²,

Xia³

et al. 2022

DMSO

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Objective The aim of this study was to investigate the effects of moderate treadmill exercise on gut microbiota, expression of proteins associated with gut barrier and to elucidate the mechanisms underlying their role in high-fat-diet-induced obese mice. Methods Six-week-old male C57BL/6 mice were randomly divided into standard chow diet control group (SD + Sed, n=6), chow diet exercise group (SD + Exe, n=6), high-fat diet control group (HFD + Sed, n=6) and high-fat diet exercise group (HFD + Exe, n=6). Exercise groups were trained on a motorized treadmill for 45 min/d at running speeds of 12 m/min, 5 days/week, for 12 consecutive weeks. The body weight and fasting blood glucose of the mice were recorded before euthanasia. Thereafter, the mice were sacrificed and the alteration of adipose mass, colonic histopathology, gut microbiome and gut barrier-related molecules were tested. Results It was found that the moderate treadmill exercise prevented the development of adiposity and hyperglycemia and effectively improved the loss of diversity and the relative abundance of intestinal microflora induced by high-fat diet. Moreover, regular exercise reversed the intestinal pathology and elevated the number of goblet cells in obesity. Besides, compared with the sedentary obese mice, the protein expression levels of colonic ZO-1 and occludin were enhanced and AMPK/CDX2 signaling pathway was significantly upregulated in obese mice that underwent exercise. Conclusion Long-term moderate treadmill exercise can markedly reduce the degree of obesity, modulate the colonic gut microbiota, and effectively activating AMPK/CDX2 signaling pathway to improve intestinal barrier in obese mice induced by high-fat diet.

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“…Western diets rich in saturated fats have been reported to cause a marked change in the functionality of the mucus barrier. In this context, reduction in fucosylated and sulfated residuals in the goblet cells of villi was observed in mice fed with a high fat diet during 25 weeks [67]. Another study revealed that a prolonged high fat diet leads to ER and oxidative stress in goblet cells reducing the production and secretion of mucins needed for a protective barrier [68].…”

Section: Dietary Modulationmentioning

confidence: 98%

Mucins Dynamics in Physiological and Pathological Conditions

Melhem

Regan-Komito

Niess

2021

IJMS

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Maintaining intestinal health requires clear segregation between epithelial cells and luminal microbes. The intestinal mucus layer, produced by goblet cells (GCs), is a key element in maintaining the functional protection of the epithelium. The importance of the gut mucus barrier is highlighted in mice lacking Muc2, the major form of secreted mucins. These mice show closer bacterial residence to epithelial cells, develop spontaneous colitis and became moribund when infected with the attaching and effacing pathogen, Citrobacter rodentium. Furthermore, numerous observations have associated GCs and mucus layer dysfunction to the pathogenesis of inflammatory bowel disease (IBD). However, the molecular mechanisms that regulate the physiology of GCs and the mucus layer remain obscured. In this review, we consider novel findings describing divergent functionality and expression profiles of GCs subtypes within intestinal crypts. We also discuss internal (host) and external (diets and bacteria) factors that modulate different aspects of the mucus layer as well as the contribution of an altered mucus barrier to the onset of IBD.

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High-fat Diet Alters the Glycosylation Patterns of Duodenal Mucins in a Murine Model

Cited by 20 publications

References 48 publications

Changes in dietary fiber intake in mice reveal associations between colonic mucin O-glycosylation and specific gut bacteria

Changes in dietary fiber intake in mice reveal associations between colonic mucin O-glycosylation and specific gut bacteria

Moderate Treadmill Exercise Modulates Gut Microbiota and Improves Intestinal Barrier in High-Fat-Diet-Induced Obese Mice via the AMPK/CDX2 Signaling Pathway

Mucins Dynamics in Physiological and Pathological Conditions

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