Seasonal size distribution of airborne culturable bacteria and fungi and preliminary estimation of their deposition in human lungs during non-haze and haze days

Gao, Min; Jia, Ruizhi; Qiu, Tian; Han, Min; Yuan, Sheng; Wang, Xüming

doi:10.1016/j.atmosenv.2015.08.004

Cited by 95 publications

(41 citation statements)

References 30 publications

(1 reference statement)

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“…Kim et al [28] reported that the airborne bacteria sizes smaller than 3.3 μm (stage 4 to 6) accounted for 40% of the total inside the nursery pig house, which was a little higher than our result. As reported, in Beijing city, the percentages of airborne culturable bacteria and fungi at ne particle sizes (stage 4 to 6) ranged from 15.34% to 45.95% and from 32.0% to 63.81% in winter, respectively, which was similar with our results [29].…”

Section: Discussionsupporting

confidence: 93%

The Distribution Characteristic of Microbial Aerosols Inside a Nursery Pig House and the Respiratory Tract of Piglets

Tang

Huang

Liu

et al. 2020

Preprint

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Abstract Background: The particulate matter (PM) is a carrier of many substances. Microorganisms are vital constituents contained in PM and the kinds and concentration are closely connected to human health and animal production. This study was aimed to investigate the distribution characteristics of microbial aerosols inside the pig house and as well as in the respiratory tract of pigs. The environment inside a nursery pig house was monitored in winter, including temperature, relative humidity, TSP, PM10, PM2.5, NH3, CO2, CO and NO. The concentrations of airborne culturable bacteria, fungi and Escherichia coli were detected. Then 16 S rRNA sequencing technology was applied to identify bacteria contained in different sized PM and the bacteria in the respiratory tract of piglets.Results: The results showed that the concentration of airborne culturable bacteria inside the pig house was significantly higher than that outside. The concentration of airborne culturable bacteria was reduced with the decrease of the size of PM; the concentration of airborne culturable fungi was mostly distributed in the size of 2.1-3.3 μm and 1.1-2.1 μm PM; most airborne culturable Escherichia coli were distributed in the size of >7.0 μm and 2.1-3.3 μm. Besides, these three types of microbial aerosols did not exhibit significant change during different time points. The 16 S rRNA results showed that the bacteria contained in PM had high similarity with that in the respiratory tract of pigs. The bacteria assemblage in the size of 1.1-3.3 μm PM had high similarity with that in the lower respiratory tract (bronchus and lung) of pigs. In addition, four potential pathogenic bacteria genera (Escherichia-Shigella, Streptococcus, Acinetobacter, Pseudomonas) were identified in the PM samples and the respiratory tract.Conclusions: These results will provide a significant scientific basis in exploring the potential risk of aerosol from animal houses for human and animal health.

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Section: Discussionsupporting

confidence: 93%

The Distribution Characteristic of Microbial Aerosols Inside a Nursery Pig House and the Respiratory Tract of Piglets

Tang

Huang

Liu

et al. 2020

Preprint

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“…In addition, endotoxin, an important bacterial membrane component, was also shown to be twice that on clear days(Wei et al, 2016). In other studies, culturable bacterial levels were shown to be higher during the haze periods than those during clear days(Liu et al, 2018;Gao et al, 2016;Gao et al, 2015a;Cao et al, 2014). Among the bacterial genera, Bacillales, Actinomycetales, Pseudomonadales were detected to dominate the community(Cao et al, 2014;Pöschl et al, 2010).…”

Section: Introductionmentioning

confidence: 67%

Time- and size-resolved bacterial aerosol dynamics in highly polluted air: new clues for haze formation mechanism

Zhang

Wang

et al. 2019

Preprint

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27Aerosol chemistry is often studied without considering microbial involvements. 28 Here, we have applied a high-volume (1 m 3 /min) aerosol sampler and the Orifice Uniform Deposit Impactor (NanoMoudi) along with molecular and microscopic 30 methods to investigate time-and size-resolved bacterial aerosol dynamics in air. Under 31 high particulate matter (PM) polluted episodes, bacterial aerosols were detected to 32 have a viability up to 50-70% in the 0.56-1 μm size range, at which elevated levels of 33 SO4 2-, NO 3and NH 4+ were concurrently observed. Engineered or acclimated for both 34 pharmaceuticals and wastewater treatment, bacteria such as Psychrobacter spp., 35Massilia spp., Acinetobacter lwoffii, Exiguobacterium aurantiacum, and Bacillus 36 megaterium were shown to have experienced massive abundance shifts in polluted air 37 on early mornings and late afternoons, on which were previously reported to witness 38 rapid new particle formation events. For example, Acinetobacter spp. were shown to 39 account for > 96% abundance at a corresponding PM2.5 level of 208 μg/m 3 . The 40 bacterial aerosol changes corresponded to the observed PM2.5 mass peak shift from 41 3.2-5.6 μm to the high viability size range of 0.56-1μm. Additionally, it is interesting 42 that elevated levels of soluble Na, Ca, Mg, K, Al, Fe and P elements that are required 43 for bacterial growth were observed to co-occur with those significant bacterial aerosol 44 structure shifts in the air. For particular time-resolved PM2.5 pollution episodes, 45 Acinetobacter and Massilia were shown to alternate in dominating the time-resolved 46 aerosol community structures. The results from a HYSPLIT trajectory model simulation 47 suggested that the role by air mass transport in affecting the observed bacterial 48 3 aerosol dynamics could be minor. As an evidence, we found that Acinetobacter, 49 Psychrobacter, Exiguobacterium, and Bacillus genera were emitted into the air with a 50 level of > 3000 CFU/m 3 from a pharmaceutical plant. In addition, high level of VOCs up 51 to 15,030 ppbv, mainly Acetone (61%) and Acetaldehyde (11%), were also detected in 52 the air inside the plant. All the data including size-resolved viability and time-resolved 53 bacterial aerosol dynamics together with their growth conditions detected in the air 54 suggested that airborne bacteria in the size range of 0.56-1μm could have played 55 important roles for haze formation in Beijing. The results about time-and size-resolved 56 bacterial aerosol dynamics from this work provide a fresh understanding of aerosol 57 chemistry especially in highly polluted air. It is hoped that these findings could lend a 58 support in future cost-effective air pollution control practices. 59 60 Keywords: Bacterial aerosol dynamics in the air, Particulate matter, Size-and Time-61 resolved bacterial aerosol, Aerosol chemistry 62 63

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“…It is demonstrated that, the concentration of both airborne bacteria and fungi were lower in heavy haze days than during non-haze days (Gao et al, 2015). With respect to increasing CO2 level, when accompanied with drought it will have a positive effect on soil fungal communities particularly on pathogenic strains such as Fusarium and thus indirect effect on their airborne spores (Curlevski et al, 2014).…”

Section: Air Pollutionmentioning

confidence: 96%

Dynamics (seasonal and intra-diurnal) of air-borne fungal spore population of Doha area, Qatar

Fayad

2016

Qatar University Life Science Symposium 2016: Biodiversity, Sustainability and Climate Change, With Perspectives From Qatar

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Seasonal size distribution of airborne culturable bacteria and fungi and preliminary estimation of their deposition in human lungs during non-haze and haze days

Cited by 95 publications

References 30 publications

The Distribution Characteristic of Microbial Aerosols Inside a Nursery Pig House and the Respiratory Tract of Piglets

The Distribution Characteristic of Microbial Aerosols Inside a Nursery Pig House and the Respiratory Tract of Piglets

Time- and size-resolved bacterial aerosol dynamics in highly polluted air: new clues for haze formation mechanism

Dynamics (seasonal and intra-diurnal) of air-borne fungal spore population of Doha area, Qatar

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