Seasonal Incidence and Molecular Characterization of<i>Salmonella</i>from Dairy Cows, Calves, and Farm Environment

Pangloli, Philipus; Dje, Yobouet; Ahmed, Omaima; Doane, C.A.; Oliver, S. P.; Draughon, F. A.

doi:10.1089/fpd.2008.0048

Cited by 18 publications

(6 citation statements)

References 21 publications

(23 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, it is known that cattle kept in a hospital settings are at risk of salmonellosis due to the presence of several predisposing factors, such as affected gut microbiota by antimicrobial and proton bomb inhibitors administration, concurrent diseases and status of immunossuppression (EWART et al, 2001;CUMMINGS et al, 2009). Additionally to these risk factors, previous reports have also shown a higher fecal shedding of Salmonella spp among hospitalized and dairy cattle in the summer and fall (PANGLOLI et al, 2008;CUMMINGS et al, 2009). Interestingly, the outbreak of Salmonella Typhimurium described in the present study occurred during the summer months in Brazil, corroborating with previous researches.…”

Section: Resultssupporting

confidence: 92%

Outbreak of multidrug resistant Salmonella Typhimurium in calves at a veterinary hospital in Brazil

et al. 2019

View full text Add to dashboard Cite

The present study aimed to describe and characterize a nosocomial outbreak caused by a multidrug resistant Salmonella Typhimurium in hospitalized calves at a veterinary medical teaching hospital from Brazil. Sixty-three (96.9%) calves showed lethargy, hyperthermia and profuse diarrhea and despite treatment, 26 (41.2%) animals died. Five animals were necropsied and stool samples of six calves were collected. The isolated strains were subjected to antimicrobial susceptibility test by disc-difusion method and were fingerprinted by ERIC-PCR. Macroscopic lesions suggestive of salmonellosis, such as fibrinonecrotic enteritis and hepatosplenomegaly were observed. Salmonellosis was confirmed by isolation of S. Typhimurium from stool samples and organs from seven affected animals. Six out of seven isolates of S. Typhimurium, exhibited 100% of similarity at ERIC-PCR, suggesting occurrence of nosocomial transmission of S. Typhimurium among the hospitalized calves. All but one S. Typhimurium isolated were resistant to marbofloxacin, enrofloxacin, florfenicol, oxytetracycline and trimethoprim/sulfamethoxazole, antimicrobial agents largely used for humans and animal treatment. This is the first study of a nosocomial outbreak of multidrug resistant S. Typhimurium in a veterinary hospital in Brazil and highlighted the need for preventive measures to reduce the risks for inpatients and humans in contact with animals.RESUMO: O objetivo do presente estudo é descrever e caracterizar um surto nosocomial provocado por S. Typhimurium multirresistente em bezerros hospitalizados em um hospital escola de medicina veteriária localizado no Brasil. Sessenta e três (96,9%) bezerros apresentaram letargia, hipertermia e diarreia profusa e, apesar do tratamento, vinte e seis animais (41,2%) morreram. Cinco animais foram necropsiados e amostras fecais de seis bezerros foram coletadas. As estirpes isoladas foram submetidas a testes de susceptibilidade a antimicrobianos pelo método de disco-difusão e foram genotipadas pelo ERIC-PCR. Lesões macroscópicas sugestivas de salmonelose, como enterite fibrinonecrótica e hepatoesplenomegalia, foram observadas. Salmonelose foi confirmada pelo isolamento de S. Typhimurium em amostras fecais e órgãos de sete animais. Dos sete isolados, seis apresentaram 100% de similaridade ao ERIC-PCR, sugerindo ocorrẽncia de transmissão nosocomial de S. Typhimurium entre os bezerros hospitalizados. Com excessão de uma estirpe, todas foram resistentes a marbofloxacina, enrofloxacina, florfenicol, oxitetraciclina e trimetoprima/sulfametoxazol, agentes antimicrobianos amplamente utilizados para o tratamento humano e animal. Esse é o primeiro estudo que demonstra um surto nosocomial de estirpes de S. Typhimurium resistentes a múltiplas drogas em um hospital veterinário no Brasil, enfatizando a necessidade de medidas preventivas que reduzam os riscos aos animais hospitalizados e a pessoas que entrarem em contato com esses animais.

show abstract

Section: Resultssupporting

confidence: 92%

Outbreak of multidrug resistant Salmonella Typhimurium in calves at a veterinary hospital in Brazil

et al. 2019

View full text Add to dashboard Cite

show abstract

“…samples being positive in district D. G. Khan. The results of present study are in agreement with the finding of Pangloli et al [22] who isolated 40-92% Salmonella spp. from animal and environment samples.…”

Section: Discussionsupporting

confidence: 94%

Prevalence and antibiotic resistance of Salmonella spp. in South Punjab-Pakistan

Qamar

Ismail

2020

PLoS ONE

View full text Add to dashboard Cite

Present study aimed at investigating the magnitude of the prevalence and antibiotic resistance among four Salmonella spp. i.e., S. typhi, S. paratyphi A, S. paratyphi B and S. typhimurium. Raw milk and environment samples were collected from the five districts of southern part of the province of Punjab in Pakistan i.e., Multan, Bahawalpur, Lodhran, Dera Ghazi Khan and Muzaffargarh. Extent of antibiotic resistance was also determined and classified as resistant, intermediate and susceptible. District–wise prevalence data on Salmonella spp. in milk and environmental samples indicated higher S. typhi, S. paratyphi B and S. typhimurium count in Bahawalpur, D.G. Khan and Muzaffargarh districts, respectively. Amongst 13 tested antibiotics, chloramphenicol and ofloxacin were found to be the most susceptible against Salmonella spp. Increased emergence of antibacterial resistance was noted with respect to the type of antibiotics among Salmonella spp. isolates. The study suggests serious interventions to be practiced by the farmers and raw milk vendors in animal husbandry and milk marketing, respectively to curb the burden of Salmonella spp. prevalence in milk. Further, active engagement of animal health division and enforcement agencies to ensure sagacious use of antibiotics at farm level may also help in containment of antimicrobial resistance in Salmonella spp.

show abstract

“…In soils and manures, warmer temperatures may facilitate survival. For instance, a survey of east Tennessee dairy farms found that Salmonella was more readily isolated from animal and environmental samples as ambient temperatures increased [ 31 ]. The current study was originally designed to examine seasonality, but farm participation and sample availability impacted when litter could be collected.…”

Section: Discussionmentioning

confidence: 99%

The prevalence and concentration of Salmonella enterica in poultry litter in the southern United States

et al. 2022

View full text Add to dashboard Cite

Poultry litter is applied to crop production land in the southern United States as a waste management strategy as it is a nitrogen-rich fertilizer and plentiful throughout the region. While litter is a known reservoir for human enteric pathogens including Salmonella enterica, little is known regarding pathogen prevalence, concentration, and common serotypes within the material. Litter from thirteen farms across four southern states was examined for Salmonella. Samples (n = 490) from six of the thirteen (46.2%) farms tested positive. Thirty-three samples out of 490 (6.7%) were Salmonella positive. Salmonella was ca. 95% less likely to be collected from stacked litter piles than from the poultry house floor or pasture, and every day increase in litter age reduced the likelihood of recovering Salmonella by 5.1%. When present, concentrations of Salmonella in contaminated poultry litter were variable, ranging from <0.45 to >280,000 MPN/g. The most prevalent serotypes found were Kentucky (45.5%), Kiambu (18.2%), and Michigan (12.1%). Salmonella Kentucky also had the greatest distribution and was found on 4 of the 6 (66.7%) positive farms. Results from this survey demonstrated that Salmonella prevalence and concentration in poultry litter is highly variable, and good agricultural practices are critical to safely use poultry litter as a soil amendment on fresh produce fields.

show abstract

Seasonal Incidence and Molecular Characterization ofSalmonellafrom Dairy Cows, Calves, and Farm Environment

Cited by 18 publications

References 21 publications

Outbreak of multidrug resistant Salmonella Typhimurium in calves at a veterinary hospital in Brazil

Outbreak of multidrug resistant Salmonella Typhimurium in calves at a veterinary hospital in Brazil

Prevalence and antibiotic resistance of Salmonella spp. in South Punjab-Pakistan

The prevalence and concentration of Salmonella enterica in poultry litter in the southern United States

Contact Info

Product

Resources

About