Seasonal effect on Brazilian propolis antibacterial activity

Sforcin, José Maurício; Júnior, Ary Fernandes; Lopes, C. A.; Bankova, Vassya; Funari, Sílvia Regina Cunha

doi:10.1016/s0378-8741(00)00320-2

Cited by 399 publications

(318 citation statements)

References 9 publications

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“…Furthermore, Jordanian propolis can effectively eliminate Enterococcus faecalis from infected dentin models ex vivo (19). Current studies involving propolis had used different microbiological methods, such as agar dilution, agar well diffusion, agar disc diffusion techniques, as well as several concentrations of the propolis extract, which in general ranged from 11% to 30% weight to volume (4,6,14,16,18,19). Nevertheless, as the present study used standardized propolis and clinical microbial samples, these materials could be further tested in future investigations employing different methods.…”

Section: Discussionmentioning

confidence: 99%

“…Scientific research has revealed its antioxidant, antibacterial, antifungal, antiviral, antiinflammatory, anti-tumor and immunomodulating properties (4). Studies on propolis applications have increased because of its therapeutic and biological properties (5,6). Current research involving propolis in dentistry spans many fields and highlights its antimicrobial and antiinflammatory activities, particularly in cariology, oral surgery, pathology, periodontics and endodontics (5,(7)(8)(9).…”

Section: Introductionmentioning

confidence: 99%

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In vitro antimicrobial activity of endodontic pastes with propolis extracts and calcium hydroxide: a preliminary study

et al. 2008

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The purpose of this study was to evaluate the antimicrobial activity of two experimental pastes containing propolis extract associated with calcium hydroxide against polymicrobial cultures collected from 16 necrotic and fistulae root canals in primary molars of 4-8-yearold children of both sexes. The agar-well diffusion technique was used to determine the antimicrobial activity of the following pastes: 11.0% ethanolic extract of propolis (EEP) + calcium hydroxide; and 11.0% extract of propolis without ethanol (EP) + calcium hydroxide. EEP, EP and the association of calcium hydroxide and propylene glycol (CHP) was used as the positive control groups, and propylene glycol was used as a negative control group. Friedman and Wilcoxon tests were used to compare the data from the microbial growth inhibition zones (p<0.05). Paste 1 and Paste 2 showed larger growth inhibition zones against microorganisms from root canal samples than CHP (p=0.021 and 0.003, respectively). Paste 2 tended to have larger growth inhibition zones than Paste 1 (p=0.053). The association between propolis and calcium hydroxide was effective in controlling dental infections in vitro.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vitro antimicrobial activity of endodontic pastes with propolis extracts and calcium hydroxide: a preliminary study

et al. 2008

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“…10 Among tropical countries, Brazil has the highest chemical diversity of propolis types, such as those that contain phenylpropanoids, prenylated phenylpropanoids (e.g., artepillin C), and sesqui-and diterpenoids, 11 which confer immunostimulatory, antioxidant, anti-inflammatory, and antimicrobial properties, respectively. 12 Studies have shown the potential of propolis against resistant microorganisms. 13,14,15 Thus, this study aimed to investigate the potential antimicrobial activity of brown propolis from the Brazilian Cerrado with or without calcium hydroxide paste as an intracanal medication against E. faecalis.…”

Section: Introductionmentioning

confidence: 99%

In vitro effectiveness of Brazilian brown propolis against Enterococcus faecalis

et al. 2015

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The aim of this study was to evaluate the in vitro antimicrobial activity of Brazilian brown propolis as an intracanal medication against Enterococcus faecalis. Thirty dentin discs prepared from intact freshly extracted bovine maxillary central incisors were infected with E. faecalis for 21 days. The specimens were distributed into six groups according to the medicament used as follows: G1-calcium hydroxide paste; G2-Carbowax 400 (control group); G3-20% brown propolis paste; G4-40% brown propolis paste; G5-20% brown propolis paste + calcium hydroxide paste; and G6-40% brown propolis paste + calcium hydroxide paste. The experimental pastes were placed into the canal lumen and left for 14 days. After each period, irrigation was performed with sterile saline to remove the medicament, and the canals were dried with sterile paper points. The dentin chips were removed from the canals with sequential sterile round burs at low speed and were immediately collected in separate test tubes containing BHI broth. The tubes were incubated at 37°C, and microbial growth was analyzed by spectrophotometry after 15 days. All the experimental medications significantly reduced the number of viable bacteria. The G4 and G5 pastes were more effective than the G1 paste, with 35.8%, 41%, and 21.3% antibacterial activity, respectively. Brazilian brown propolis shows antibacterial capacity against E. faecalis.

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“…This product has attracted researchers' interest in the last decade because of its numerous biological and pharmacological properties, such as antimicrobial (Sforcin et al, 2000;Freitas et al, 2006;Búfalo et al, 2009), antitumoral (Bazo et al, 2002), immunomodulatory (Sforcin, 2007), anti-inflammatory (Silva et al, 2012), antioxidant (Kumazawa et al, 2010), among others.…”

Section: Introductionmentioning

confidence: 99%

Propolis and its constituent caffeic acid suppress LPS-stimulated pro-inflammatory response by blocking NF-κB and MAPK activation in macrophages

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et al. 2013

Journal of Ethnopharmacology

153

102

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Propolis and its constituent caffeic acid suppress LPS-stimulated pro-inflammatory response by blocking NF-κB and MAPK activation in macrophages, Journal of Ethnopharmacology, http://dx.doi.org/ 10. 1016/j.jep.2013.06.004 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractEthnopharmacological relevance: Propolis is a bee product with numerous biological and pharmacological properties, such as immunomodulatory and anti-inflammatory activities.It has been used in folk medicine as a healthy drink and in food to improve health and prevent inflammatory diseases. However, little is known about its mechanism of action.Thus, the goal of this study was to verify the antioxidant activity and to explore the antiinflammatory properties of propolis by addressing its intracellular mechanism of action.Caffeic acid was investigated as a possible compound responsible for propolis action. Materials and Methods:The antioxidant properties of propolis and caffeic acid were evaluated by using the 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH) scavenging method. To analyze the anti-inflammatory activity, Raw 264.7 macrophages were treated with different concentrations of propolis or caffeic acid, and nitric oxide (NO) production, a strong pro-inflammatory mediator, was evaluated by the Griess reaction. The concentrations of propolis and caffeic acid that inhibited NO production were evaluated on intracellular signaling pathways triggered during inflammation, namely p38 mitogenactivated protein kinase (MAPK), c-jun NH 2 -terminal kinase (JNK1/2), the transcription nuclear factor (NF)-NB and extracellular signal-regulated kinase (ERK1/2), through Western blot using specific antibodies. A possible effect of propolis on the cytotoxicity of hepatocytes was also evaluated, since this product can be used in human diets.Results: Caffeic acid showed a higher antioxidant activity than propolis extract. Propolis and caffeic acid inhibited NO production in macrophages, at concentrations without cytotoxicity. Furthermore, both propolis and caffeic acid suppressed LPS-induced signaling pathways, namely p38 MAPK, JNK1/2 and NF-NB. ERK1/2 was not affected by propolis extract and caffeic acid. In addition, propolis and caffeic acid did not induce hepatotoxicity at concentrations with strong anti-inflammatory potential. Conclusions:Propolis exerted an antioxidant and anti-inflammatory action and caffeic acid may be involved in its inhibitory effects on NO production and intracellular signaling cascades, suggesting its use as a natural source of safe anti-inflammatory drugs.

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Seasonal effect on Brazilian propolis antibacterial activity

Cited by 399 publications

References 9 publications

In vitro antimicrobial activity of endodontic pastes with propolis extracts and calcium hydroxide: a preliminary study

In vitro antimicrobial activity of endodontic pastes with propolis extracts and calcium hydroxide: a preliminary study

In vitro effectiveness of Brazilian brown propolis against Enterococcus faecalis

Propolis and its constituent caffeic acid suppress LPS-stimulated pro-inflammatory response by blocking NF-κB and MAPK activation in macrophages

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