Seasonal dynamics in a coastal Vibrio community examinedby a rapid clustering method based on 16S rDNA

Maeda, Takao; Matsuo, Yuka; Furushita, Manabu; Shiba, Tsuneo

doi:10.1046/j.1444-2906.2003.00633.x

Cited by 28 publications

(29 citation statements)

References 37 publications

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“…One of the lowest, 0.002 to 0.015%, has been recorded in the Baltic Sea using culture-independent quantitative competitive PCR (Eiler et al 2006), and the highest, 68%, on the Japanese coast was detected by culture-dependent techniques (Simidu & Tsukamoto 1980). Vibrios can be found, but are seldom dominant, in marine sediments (Oliver et al 1982, Maeda et al 2003. Indeed, the facultative anaerobic growth of vibrios suggests a possible commensal role in mediating organic matter decomposition.…”

Section: Discussionmentioning

confidence: 99%

Association between phytoplankton and Vibrio spp. along the southwest coast of India: a mesocosm experiment

Rehnstam-Holm¹,

Godhe²,

Härnström³

et al. 2010

Aquat. Microb. Ecol.

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We report the results from a mesocosm study investigating the interrelationship between microalgae and vibrios. The mesocosms were inoculated with plankton, plankton + sediment, or sediment. We followed the diatom bloom and increases in the abundance of Vibrio spp. and V. parahaemolyticus in conjunction with several environmental variables in all mesocosms and at a reference site. The dominating diatom genera were also identified. Temperature, salinity, and pH were nearly invariant in the mesocosms and did not contribute to the results. The principal environmental variables that correlated to vibrio abundance were total bacterial plate counts, phosphorus and ammonia (positive relationship), and oxygen and silica (negative). Nitrate, total bacterial counts and chlorophyll a (chl a) did not correlate with vibrio growth. The highest diatom abundances were followed by increases in vibrios in all mesocosms. This was also observed in field sampling. Together, these results suggest that diatom blooms could support Vibrio spp. growth. V. parahaemolyticus was initially favoured by sediment. The contribution of V. parahaemolyticus to the total bacterial population was low, on average 0.5%, but constituted a rather high proportion of the vibrio population in the mesocosm systems, i.e. on average 18%. Some of the identified diatom genera, e.g. Chaetoceros and Skeletonema, were negatively correlated to vibrios, while Coscinodiscus was positively correlated. The results indicate that phytoplankton blooms, when recorded as high levels of chl a, should be used with caution as predictors for future vibrio epidemics, since the origin of the chl a might have a significant effect on vibrio abundance. KEY WORDS: Mesocosm · Phytoplankton blooms · Vibrio spp. Resale or republication not permitted without written consent of the publisher OPEN PEN

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Section: Discussionmentioning

confidence: 99%

Association between phytoplankton and Vibrio spp. along the southwest coast of India: a mesocosm experiment

Rehnstam-Holm¹,

Godhe²,

Härnström³

et al. 2010

Aquat. Microb. Ecol.

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“…A role for V. splendidusrelated strains in the mortality of mollusks and fish was also reported (6, 9, 13). V. splendidus and related species are widespread in marine environments (14,30,32,34,35). Recently, two genetically distinctive populations of V. splendidus-related strains from North Atlantic coastal water have been distinguished.…”

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confidence: 99%

Mutation is the Main Driving Force in the Diversification of the Vibrio splendidus Clade

et al. 2009

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The Vibrio splendidus clade is the biggest in Vibrionales composed of 11 described species (25). Diversification of these species may have occurred 260 million years ago. The main driving forces of speciation in this clade have never been studied. Population biological parameters (population base recombination rate (ρ), population base mutation rate (θ), and index of association (Ia)) were determined among 16 strains of 9 defined species in the Splendidus cluster. A comparison of individual gene phylogeny indicated significant incongruence in tree topology, which suggests the occurrence of recombination between species. Homologous recombination between species was detected at four loci. However, the mutation rate θ was higher than the recombination rate ρ, suggesting that mutation is the main driving force in the diversification of V. splendidus-related species.Key words: diversification, population biology, vibrios, Vibrio splendidus, multilocus sequence analysis Vibrio splendidus was originally described by Beijerinck in 1900 as a luminous marine bacterium (1,2,22). The taxonomic status of this species was confirmed through DNA-DNA hybridization by Reichelt et al. (22). V. splendidusrelated strains isolated from marine environments remained in focus due to the psychrophilic nature of these vibrios in the global warming era (27,34,35). A role for V. splendidusrelated strains in the mortality of mollusks and fish was also reported (6, 9, 13). V. splendidus and related species are widespread in marine environments (14,30,32,34,35). Recently, two genetically distinctive populations of V. splendidus-related strains from North Atlantic coastal water have been distinguished. Bacteria of these two populations differed in range of temperature for growth and had less than 1% of the 16S rRNA gene sequence heterogeneity (30,32).Because V. splendidus-related species share niches in the environment, including the gut and tissue of bivalves, it would be reasonable to assume that horizontal gene transfer and recombination are important forces leading to diversification in this group. The multilocus sequence analysis (MLSA) is a reliable tool for both inferring the taxonomic backbone of species groups and estimating the relative contributions of mutation and recombination (31,33,36). Our recent MLSA clearly separated the V. splendidus clade from all other recognized species of vibrios (25). However, V. splendidus-related species are genetically and phenotypically similar (29). Overall, the species have more than 30% reciprocal DNA-DNA similarity, 90.6-96.5% nucleotide sequence similarity of MLSA concatenated genes and 96.5-99.8% average amino acid identity at gene loci, but a rather broad GC content. The high genomic similarity might suggest that these species readily exchange DNA at conserved loci. To test this hypothesis, we analyzed the relative contribution of mutation and recombination to the diversification using an extended set of strains belonging to the Splendidus group by means of MLSA. Materials and Methods Bacteria...

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“…The low abundance of S. hanedai and V. splendidus and the lack of V. logei, P. phosphoreum, V. orientalis, and S. woodyi recorded in this study could be explained by the psychrophilic nature of these species, which have optimum growth at temperatures lower than 18 ºC and thus live better in cold waters (Nishiguchi 2000, Kato and Nogi 2001, Maeda et al 2003. The frequency of luminous species was similar at all sites (table 4).…”

Section: Discussionmentioning

confidence: 38%

“…La baja abundancia de S. hanedai y V. splendidus y la ausencia de V. logei, P. phosphoreum, V. orientalis y S. woodyi en el presente estudio podrían explicarse por la naturaleza psicrofílica de estas especies, las cuales tienen un crecimiento óptimo a temperaturas inferiores a 18 ºC y consecuentemente viven mejor en aguas frías (Nishiguchi 2000, Kato y Nogi 2001, Maeda et al 2003. La frecuencia de especies luminosas fue similar en los tres sitios (tabla 4).…”

Section: Discussionunclassified

Phenotypic characterization of culturable marine luminous bacteria isolated from coastal waters of the southern Adriatic Sea (Otranto, Italy)

et al. 2012

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ABSTRACT. To determine the abundance and species composition of luminous bacteria in the coastal waters of the southern Adriatic Sea (Otranto, Italy), samples were collected from three sites affected by different degrees of anthropogenic pollution. A total of 116 strains of marine luminous bacteria were isolated and subjected to phenotypic characterization, which included 36 biochemical tests. Numerical analysis of the data revealed five clusters with > 86% similarity, which were readily identified as Photobacterium leiognathi, Vibrio fischeri, Vibrio harveyi, Vibrio splendidus biovar I, and Shewanella hanedai. The relative abundance of each species shows that culturable luminous bacteria in the monitored waters are composed mainly of V. harveyi (69.0%), followed by V. fischeri (13.8%) and P. leiognathi (12.1%). In addition, human activity appears to affect the total abundance of luminous bacteria but not the selection of individual species, since the frequencies of the bacteria were similar at all examined sites.Key words: luminous bacteria, seawater, phenotypic characterization, coastal waters, heterotrophic bacteria. RESUMEN. Con el fin de determinar la abundancia y composición de especies de bacterias luminosas en aguas costeras del sur del marAdriático (Otranto, Italia), se recolectaron muestras en tres sitios con diferente grado de contaminación antropogénica. Se aislaron 116 cepas de bacterias luminosas marinas y se sometieron a 36 pruebas bioquímicas para su caracterización fenotípica. El análisis numérico de los datos mostró cinco grupos con > 86% de similitud, los cuales fueron identificados como Photobacterium leiognathi, Vibrio fischeri, Vibrio harveyi, Vibrio splendidus biovar I y Shewanella hanedai. La abundancia relativa de cada especie muestra que las bacterias luminosas cultivables en las aguas analizadas consisten principalmente de V. harveyi (69.0%), V. fischeri (13.8%) y P. leiognathi (12.1%). Además, la actividad humana parece afectar la abundancia total de bacterias luminosas pero no la selección de especies individuales, ya que las frecuencias de las bacterias fueron similares en todos los sitios estudiados.Palabras clave: bacterias luminosas, agua marina, caracterización fenotípica, aguas costeras, bacterias heterótrofas.

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Seasonal dynamics in a coastal Vibrio community examinedby a rapid clustering method based on 16S rDNA

Cited by 28 publications

References 37 publications

Association between phytoplankton and Vibrio spp. along the southwest coast of India: a mesocosm experiment

Association between phytoplankton and Vibrio spp. along the southwest coast of India: a mesocosm experiment

Mutation is the Main Driving Force in the Diversification of the Vibrio splendidus Clade

Phenotypic characterization of culturable marine luminous bacteria isolated from coastal waters of the southern Adriatic Sea (Otranto, Italy)

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