Seasonal changes in antioxidant level of Scots pine (Pinus sylvestris L.) needles exposed to industrial pollution. II. Enzymatic scavengers activities

Pukacka, Stanisława; Pukacki, Paweł M.

doi:10.1007/s11738-000-0089-z

Cited by 19 publications

(13 citation statements)

References 41 publications

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“…1.15.11) activity was determined according to Giannopolitis and Ries (1977), by measuring its ability to inhibit the photochemical reduction of NBT, as described in Pukacka and Pukacki (2000).…”

Section: Enzyme Extraction and Assaymentioning

confidence: 99%

ROS production and antioxidative system activity in embryonic axes of Quercus robur seeds under different desiccation rate conditions

2011

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The seeds of pedunculate oak (Quercus robur L.) were subjected to slow (S) and rapid (R) desiccation at desiccation rates of 0.16 and 0.39% H 2 O per hour, respectively. Till ca. 40% water content (WC) the germination capacity of seeds in the S and R variants was high (ca. 100%). Between 40 and 28% WC, germination capacity declined to 20 and 50% in S and R variants, respectively. The decrease in seed viability was accompanied by a significant increase of electrolyte leakage from embryonic axes (28% for S and 15% for R variants). In the embryonic axes of seeds subjected to slow desiccation, malondialdehyde (MDA) and free fatty acid (FFA) contents were significantly higher than those in R variants, indicating greater membrane damage due to lipid peroxidation. The production of ROS (H 2 O 2 and O 2 Á-) was significantly higher in S than in R variants. The low molecular weight antioxidants a-tocopherol, ascorbic acid (ASA), and phenolic compounds indicated different reactions in response to desiccation stress. ASA levels decreased during desiccation to a similar degree in both the S and R variants. A significant decrease of total phenols was observed in R variant, which coincided with a significant increase of guaiacol peroxidase (POX) activity. a-Tocopherol content was significantly higher in the embryonic axes of seeds subjected to rapid drying. The activities of the enzymatic scavengers APX and GR had similar runs and were slightly higher in R variant. The activities of POX and SOD were significantly higher in the embryonic axes of seeds subjected to rapid drying. These results show that rapid dehydration of Q. robur seeds leads to the greater mobilization of antioxidant system in embryonic axes, particularly increased levels of a-tocopherol and POX and SOD activities, in the first stages of water loss. This mobilization has a greater impact on maintenance of higher viability of seeds after drying to lower level of WC.

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Section: Enzyme Extraction and Assaymentioning

confidence: 99%

ROS production and antioxidative system activity in embryonic axes of Quercus robur seeds under different desiccation rate conditions

2011

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“…1.15.11) activity was determined according to Giannopolitis and Ries (1977), by measuring its ability to inhibit the photochemical reduction of nitroblue tetrazolium (NBT), as described in Pukacka and Pukacki (2000).…”

Section: Enzyme Extraction and Assaymentioning

confidence: 99%

Production and scavenging of reactive oxygen species in Fagus sylvatica seeds during storage at varied temperature and humidity

Pukacka

Ratajczak

2005

Journal of Plant Physiology

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113

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“…Changes in membrane integrity very often result from the activity of free radicals, which may be part of pollutants or are formed in plant cells under the influence of industrial pollution (Pukacka andPukacki 2000,-Yamamoto et aL 2002).…”

Section: Discussionmentioning

confidence: 99%

Chronic environmental pollution alters adenylate levels in needles and fine roots of Scots pine

2003

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Contents of ATP, ADP, AMP, inorganic phosphate, and values of ATP/ADP ratio, adenylate energy charge (AEC), phosphorylation potential (PP) and adenylate kinase activity were analysed in needles and fine roots of Scots pine trees grown at the polluted and control (free of acute air pollution) site. Also chemical properties of the soil and mineral elements in needles from both sites were analysed. In comparison with the control, developing needles from the polluted site contained less ATP, the same amount of ADP and more AMP, and had lower values of ATP/ADP, AEC and PP. In one-year-old needles from the polluted site no change or a decrease in ATP was recorded, while ADP decreased, AMP increased, AEC did not change, and ATP/ADP ratio and PP were higher. In fine roots from the polluted site AMP level, was higher, while ATP, ADP, ATP/ADP ratio, PP and AEC were lower than in the control.

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Seasonal changes in antioxidant level of Scots pine (Pinus sylvestris L.) needles exposed to industrial pollution. II. Enzymatic scavengers activities

Cited by 19 publications

References 41 publications

ROS production and antioxidative system activity in embryonic axes of Quercus robur seeds under different desiccation rate conditions

ROS production and antioxidative system activity in embryonic axes of Quercus robur seeds under different desiccation rate conditions

Production and scavenging of reactive oxygen species in Fagus sylvatica seeds during storage at varied temperature and humidity

Chronic environmental pollution alters adenylate levels in needles and fine roots of Scots pine

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