Production and scavenging of reactive oxygen species in Fagus sylvatica seeds during storage at varied temperature and humidity

Pukacka, Stanisława; Ratajczak, Ewelina

doi:10.1016/j.jplph.2004.10.012

Cited by 113 publications

(69 citation statements)

References 46 publications

(49 reference statements)

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“…Moreover, the results showed that the loss of seed viability was associated with the accumulation of ROS, as the level of ROS was much lower in seeds with higher germination (F5 and F10) compared to that under NF (Table 2). Other research also indicated that ROS may be involved in loss of seed viability (Wilson and Mcdonald 1986;Hendry 1993;Bailly et al 1996;McDonald 1999;Pukacka and Ratajczak 2005). However, we found that the level of ROS in dormant seeds was much lower than that in non-dormant seeds (Table 3).…”

Section: Discussionmentioning

confidence: 37%

Oxidative metabolism‐related changes during germination of mono maple (Acer mono Maxim.) seeds under seasonal frozen soil

Qin

Liu

2009

Ecological Research

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Oxidative metabolism-related changes during germination of mono maple (Acer mono Maxim.) seeds under seasonal frozen soil Abstract The influence of seasonal frozen soil and buried depth on germination of mono maple (Acer mono Maxim.) seeds was studied in field conditions in winter in a sub-alpine region. Mono maple seeds almost lost their ability to germinate in non-freezing soil, while seasonal frozen soil treatments facilitated the germination accompanied with a progressive accumulation of reactive oxygen species (ROS). The result indicates that ROS may act as a positive signal for seed dormancy. However, exceeding accumulation of ROS led to decrease in germination rate. We suggest that the shift from a signaling to a deleterious role may be related to the accumulation of these ROS above a threshold level that leads to various cellular alterations and damage. The enhanced activities of antioxidant enzymes appear to be more closely related to freezing tolerance, because of their ability to scavenge ROS to avoid deleterious events. Seasonal frozen soil was beneficial in accelerating the germination of mono maple seeds. However, a slight increase of freezing temperature may have also facilitated the germination of mono maple seeds by enhanced activities of antioxidant enzymes. Hence, moderate winter warming may be beneficial to mono maple regeneration due to the improvement of seed germination, but the disappearance of seasonal frozen soil may lead to germination failure of the mono maple seeds.

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Section: Discussionmentioning

confidence: 37%

Oxidative metabolism‐related changes during germination of mono maple (Acer mono Maxim.) seeds under seasonal frozen soil

Qin

Liu

2009

Ecological Research

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“…It is known that with the initiation of drying, there is a shift in gene expression compelling the seeds to a different physiological status, where genes coding for the protective molecules play an important role (Ramanjulu and Bartels, 2002). Proteins related to desiccation tolerance and involved in the metabolic changes (Leprince et al, 2000;Avelange-Macherel et al, 2006), protection against oxidation and other putative protective molecules show a particular abundance during the desiccation (Pukacka and Ratajczak, 2005). Small heat-shock proteins (sHSPs) (Wehmeyer and Vierling, 2000) and late embryogenesis abundant (LEA) proteins (Gallardo et al, 2001;Boudet et al, 2006) are amongst the molecules with increased the abundance during drying.…”

Section: Proteome Analysis: Effect Of Drying and Drying/imbibition Trmentioning

confidence: 99%

Protein expression upon desiccation and imbibition of Magnolia ovata A. St.-Hil seeds

José

Silva

Davide

et al. 2011

Braz. arch. biol. technol.

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“…The genes associated to the desiccation tolerance, especially those involved in metabolic changes (Leprince et al, 2000;Walters et al, 2002b), protection against oxidation (Pukacka and Ratajczak, 2005), and other supposed protectant molecules have shown particular expression pattern during desiccation. The gain of desiccation tolerance of orthodox seeds during their development has been correlated to different expression of genes such as ABI3, ABI5, LEC1 (Jones et al, 1997;Koornneef et al, 1984;Parcy et al, 1994;Harada, 2001), among others.…”

Section: Introductionmentioning

confidence: 99%

“…The transketolases act in the reduction and oxidation of the pentose phosphate pathway, being responsible for the intermediary synthesis of phosphate sugar, which can be utilized in the carbohydrates metabolism, nucleic acids and amino-acids biosynthesis reactions (Racker, 1961). According to Bernacchia et al (1995), the differential expression of genic family during the rehydration phase in Craterostigma plantagineum plants suggested the hypothesis that the transketolases are able to act in the sugars conversion, the main phenomenon in the rehydration process.…”

Section: Introductionmentioning

confidence: 99%

Partial characterization of genes from the embryonic axis of Melanoxylon brauna Schott. (Leguminosae-Caesalpinioideae) seeds

Silva

Brommonschenkel

Faria

et al. 2012

Rev. bras. sementes

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-The objective of this study was to partially characterize some genes involved in the desiccation tolerance of the embryonic axis of Melanoxylon brauna seeds subjected, or not, to oven fastdrying. Seeds were initially dried rapidly in an oven at 40 ºC, 50 ºC, 60 ºC, 70 ºC, and 80 °C, for 24, 48 and 72 h and then subjected to germination tests and moisture content determination. Degenerate primers were designed for 19 genes. The CDNA was used as a template for PCR amplifications using the degenerate primers, and the PCR products obtained were purified, cloned and sequenced. The seeds showed a gradual reduction in percent germination with increasing temperature and drying time. Nucleotide sequences of the cloned fragments related to genes CAT1, SPS1, Abi5, Transk and PM25 were obtained. The similarity analysis with the sequences deposited in databases revealed similarities with genes CAT1, SPS1, Transk and PM25 from other plant species. The nucleotide sequences obtained from the respective genes will be used for designing specific primers for gene expression analyses during seed germination in order to understand the causes for loss of physiological quality of Melanoxylon brauna seeds.Index terms: water, stress, drying, sequencing.Caracterização parcial de genes do eixo embrionário de sementes de Melanoxylon brauna Schott. (Leguminosae-Caesalpinioideae) RESUMO -O presente trabalho objetivou caracterizar parcialmente os genes envolvidos com a tolerância à dessecação do eixo embrionário de sementes de Melanoxylon brauna submetidas ou não à secagem rápida. As sementes foram submetidas à secagem rápida em estufa nas temperaturas de 40 ºC, 50 ºC, 60 ºC, 70 ºC e 80 ºC, nos tempos de 24, 48 e 72 h e, em seguida, submetidas ao teste de germinação e determinação do grau de umidade. Foram utilizados primers degenerados para 19 genes. Os produtos de PCR obtidos foram purificados, clonados no vetor pGEM-T Easy e sequenciados. As sementes apresentaram redução gradativa na porcentagem de germinação com o aumento da temperatura e tempo de secagem. Foram obtidas as sequências dos fragmentos clonados referentes aos genes CAT1, SPS1, ABI5, Transk e PM25. A comparação das sequências obtidas com sequências depositadas em bancos de dados revelou similaridade com os genes CAT1, SPS1, Transk e PM25 de outras espécies. As sequências de nucleotídeos obtidas dos respectivos genes servirão para a confecção de oligonucleotídeos específicos para posteriores estudos de expressão gênica durante a germinação de sementes de M. brauna submetidas à secagem, visando entender as causas da perda de qualidade fisiológica dessas sementes.Termos para indexação: água, secagem, sequenciamento, estresse.

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Production and scavenging of reactive oxygen species in Fagus sylvatica seeds during storage at varied temperature and humidity

Cited by 113 publications

References 46 publications

Oxidative metabolism‐related changes during germination of mono maple (Acer mono Maxim.) seeds under seasonal frozen soil

Oxidative metabolism‐related changes during germination of mono maple (Acer mono Maxim.) seeds under seasonal frozen soil

Protein expression upon desiccation and imbibition of Magnolia ovata A. St.-Hil seeds

Partial characterization of genes from the embryonic axis of Melanoxylon brauna Schott. (Leguminosae-Caesalpinioideae) seeds

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