Seasonal and biological variations ofEpidendrum mosenii: quantification of 24-methylenecycloartanol using gas chromatography

Abstract

Epidendrum mosenii is a Brazilian medicinal plant, traditionally used to treat infective and dolorous processes. The present article reports a comparative study of the chemical and pharmacological aspects of different parts and seasons of this plant. The results demonstrate that 24-methylenecycloartanol (1), one of the main active principles present in this plant, is located practically in all the parts and during all seasons, but it is much more concentrated in the stems when collected in spring and summer. The pharmacological results indicate that dichloromethane extracts collected in spring and summer were the most active when evaluated against the writhing test in mice, being several times more potent than some reference drugs used as comparison. Together, the results strongly suggest that the antinociceptive effect of E. mosenii is related, at least in part, to the presence of compound 1, and this finding could be useful for quality control of phytopreparations based on this plant.

Ecological and Phytochemical Studies on Euphorbia retusa (Forssk.) from Egyptian Habitat

“…Three esterified tetracyclic triterpenes of compound 2 were recently reported from the dichloromethane extract of the roots of E. retusa : 24-methylenecycloartanyl formate, 24-methylenecycloartanyl 2′ E ,4′ E -decadienoate, and tirucalla-7,24-dien-3-yl 2′ E ,4′ E- decadienoate [ 27 ]. Biologically, compound 2 was reported to prevent diet-induced obesity in the liver through GIP-dependent and GIP-independent mechanisms [ 28 ]. Compound 2 was additionally reported to exhibit anti-inflammatory activity [ 29 ].…”

Ethnobotany, Pharmacology and Chemistry of Medicinal Orchids from Veracruz

“…24-methylene-cicloarta nol, dichloromethane extract [7], methanolic extract [18] and phenanthrenes [19][20][21].…”

“…A similar traditional use to alleviate pain is referred to China for the stems of E. mosenii [20], a related species on which pharmacological testing proved a strong anti-nociceptive effect [18][19][20]. Laboratory tests demonstrated that a dichloromethane extract, 24-methylene-cicloartanol and phenanthrenes were responsible of this action [19][20][21].…”

Produtos Naturais e Sintéticos com potencial terapêutico: 15 anos de estudos realizados no núcleo de investigações Químico-Farmacêuticas (NIQFAR)/UNIVALI

“…Além destes, foram ainda identificados duas substâncias fenólicas, identificadas como dihidroestilbenos 4,5´-dihidroxi-3,3´-dimetoxi-dihidroestilbeno (49) e 4,3´,5´-trihidroxi-3-metoxi-dihidroestilbeno (50), os quais exerceram propriedades analgésicas relevantes em camundongos (ROSA, 2005). Numa avaliação da composição química em relação à variação sazonal, observou-se que 48 está presente em todas as partes da planta, e durante todas as estações do ano, porém a maior concentração foi evidenciada nos caules coletados na primavera e no verão (ROSA et al, 2007). NIERO et al, 1999NIERO et al, , 2002.…”