Search for viral nucleic sequences in rheumatoid cells.

Norval, Mary; Smith, Charles B.

doi:10.1136/ard.38.5.456

Cited by 14 publications

(1 citation statement)

References 21 publications

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“…Labelling of the same culture with 3Hthymidine did not produce such a peak, supporting the idea that there might have been production of an RNA-containing virus by the adherent synovial cells from this patient. The density, 1 21 g/cm3, is similar to that found on 3H-uridine labelling of measles virus produced from Vero cells (Norval and Smith, 1979).…”

Section: Culture Techniquessupporting

confidence: 79%

Search for viruses in rheumatoid macrophage-rich synovial cell populations.

Hart¹,

Norval²

1980

Annals of the Rheumatic Diseases

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SUMMARY The adherent cells remaining after short-term culture of synovial fluid and synovial membrane cells from rheumatoid and non-rheumatoid patients were examined for the presence of a productive virus infection and for various viral antigens. Labelling was carried out with 3H-thymidine and 3H-uridine followed by sucrose density gradient centrifugation of the culture supernatant. Only in 1 case was there incorporation of 3H-uridine into material of density 1 .21 g/cm3. Viral antigens were tested for by indirect immunofluorescence with antisera to rubella virus, the retroviruses RD-1 14 and simian sarcoma associated virus, early adenovirus type 2 antigens, late adenovirus type 2 antigens, SV-40 T antigen, and in 1 case measles virus. No cell showed immunofluorescence with any antiserum except the early adenovirus type 2 antiserum, which stained the cytoplasm of about half the synovial cell cultures, some from rheumatoid and some from non-rheumatoid patients.

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Section: Culture Techniquessupporting

confidence: 79%