Sea fennel (Crithmum maritimum L.) under salinity conditions: a comparison of leaf and root antioxidant responses

Abstract

The present study was carried out to compare the effect of NaCl on growth, cell membrane damage, and antioxidant defences in the halophyte Crithmum maritimum L. (sea fennel). Physiological and biochemical changes were investigated under control (0 mM NaCl) and saline conditions (100 and 300 mM NaCl). Biomass and growth of roots were more sensitive to NaCl than leaves. Roots were distinguished from leaves by increased electrolyte leakage and high malondialdehyde (MDA) concentration. Superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) activities, ascorbic acid (AA) and glutathione (GSH) concentrations were lower in the roots than in the leaves of control plants. The different activity patterns of antioxidant enzymes in response to 100 and 300 mM NaCl indicated that leaves and roots reacted differently to salt stress. Leaf CAT, APX and glutathione reductase (GR) activities were lowest at 300 mM NaCl, but they were unaffected by 100 mM NaCl. Only SOD activity was reduced in the latter treatment. Root SOD activity was significantly decreased in response to 300 mM NaCl and root APX activity was significantly higher in plants treated with 100 and 300 mM compared to the controls. The other activities in roots were insensitive to salt. The concentration of AA decreased in leaves at 100 and 300 mM NaCl, and in roots at 300 mM NaCl, when compared to control plants. The concentrations of GSH in NaCl-treated leaves and roots were not significantly different from the controls. In both organs, AA and GSH were predominating in the total pool in ascorbic acid and glutathione, under control or saline conditions

Study of interaction effect between triacontanol and nitric oxide on alleviating of oxidative stress arsenic toxicity in coriander seedlings

“…The electrolyte leakage (EL) was determined as described by Ben Hamed et al (2007) in leaves' tissue of the coriander plants. The level of lipid peroxidation in coriander leaves was determined as the amount of 2-thiobarbituric acidreactive substances (TBARS) by means of malondialdehyde (MDA) and other aldehydes content formed as described by Heath and Packer (1968) and Meirs et al (1992), respectively.…”

Short-term physiological responses to copper stress in Salvinia auriculata Aubl.

“…For the calculation of MDA and other aldehydes, it was used the extinction coefficients (e) 1.55 x 10 , respectively. The electrolyte leakage (EL) was determined as described by Ben Hamed et al (2007). The plant samples (300 mg of FW) were placed in plastic test tubes containing 15 mL of ultra-pure water.…”

Antioxidant Enzyme Activities as a Tool to Discriminate Ecotypes of Crithmum maritimum L. Differing in Their Capacity to Withstand Salinity

“…This robust antioxidant activity is considered to be effective in protecting mangroves from excess irradiance (which generates ROS) under natural conditions (Cheeseman et al, 1997). Higher antioxidant defences in leaves compared to those of roots accounted for the survival of the halophyte Crithmum maritimum L. under high salinity conditions (Ben Hamed et al, 2007). In the same species, Ben Amor et al (2005) showed an increased total SOD activity in shoot tissues in presence of 50 mM NaCl stress while SOD activity in root tissues decreased upon salt treatment (up to 200 mM).…”