SDS PAGE Electrophoresis in Mustard Cultivars

Diversity Analysis and Hybridity Test of Drought Responsive Wheat Cultivars and Their F1’s on the Basis of SDS-PAGE Profiles

“…Use of biochemical markers for maintaining the genetic purity of line and hybridity of F 1 s has also been reported earlier (Bhatt and Khanna, 2006;Pallavi et al, 2010;Geetha and Balamurugan, 2011;Sharma et al, 2015). Therefore, the present investigation was undertaken to study utility of biochemical marker as an alternative method for assessment of diversity and distinguishing the F 1 s and parents.…”

Assessment of Genetic Diversity for Storage Seed Protein in a Set of Released Varieties and their Diallel Set of Crosses in Indian Mustard [Brassica juncea (L.) Czern. & Coss.] Using SDS PAGE

“…The parents / genotypes used in the study were different released varieties, therefore, it was thought justified to determine their differences among themselves on the basis of the profile of their seed storage proteins. Characterization of genotypes based on seed storage protein/subunits is well documented in different groups of crops such as blackgram (Ghafoor and Ahmad, 2005;Ghafoor et al, 2002), Capsicum annuum L. (Anu and Peter, 2003), Solanum (Menella et al, 1999), Vignaspp (Rao et al, 1992;Sharma, 2012;Chaoudhary, 2013) and Wheat (Siddiqui and Naz, 2009), including mustard (Rabbani et al, 2001;Geetha and Balamurugan, 2011;Mukhlesure and Hirata, 2004;Parashar, 2014).…”

Genetic diversity of seed storage protein in the Ethiopian garden cress (Lepidium sativum L.)

“…For extraction of proteins, mortar and pestle were used to crush and grind seeds of each genotype. Defatting of about 0.1 g of flour was carried out with chloroform, methanol and acetone in the ratio of 2:1:1 as described by Geetha and Balamurugan (2011) for mustard genotypes. Next day, about 0.05 g of flour was suspended in 1 ml extraction buffer (0.125 M Tris-Cl, 4% SDS, 20% v/v glycerol, 1% 0.2 M DTT, 25 mM EDTA, pH 6.8).…”