Scrotal heat stress causes sperm chromatin damage and cysteinyl aspartate-spicific proteinases 3 changes in fertile men

Zhang, Meihua; Zhi-Da, Shi; Yu, Jianchun; Zhang, Yanping; Wang, Leiguang; Yi, Qiu

doi:10.1007/s10815-015-0451-0

Cited by 44 publications

(47 citation statements)

References 55 publications

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“…It has been reported as inducing erectile dysfunction, premature ejaculation, orgasmic difficulty, and low sperm quality (Nathan, 1986;Ernst et al, 1993;Clarke et al, 1999;Kennedy et al, 1999;Rao et al, 2015;Zhang et al, 2015). In the recent study, all parameters of adverse male reproductive system including decreased body weight in rats exposed to restraint stress (Fig.…”

Section: Discussionmentioning

confidence: 95%

“…(1) erectile dysfunction (Nathan, 1986;Ernst et al, 1993;Kennedy et al, 1999), (2) decrease of sperm quality (Almeida et al, 1998;Clarke et al, 1999;Hari Priya and Sreenivasula Reddy, 2012;Hari Priya et al, 2014;Rao et al, 2015;Zhang et al, 2015), (3) decrease of testosterone levels (Orr and Mann, 1990;Retana-Márquez et al, 2003;Weissman et al, 2009;Lin et al, 2014;Prabsattroo et al, 2015), and (4) damage to testicular tissue (Rai et al, 2003;Aziz et al, 2013;Prabsattroo et al, 2015). Indeed, the corticosterone levels are markedly elevated under a restraint immobilization condition (Bhatia et al, 2011;Prabsattroo et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Changes of testicular phosphorylated proteins in response to restraint stress in male rats

Arun

Burawat

Sukhorum

et al. 2016

J. Zhejiang Univ. Sci. B

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Abstract:Objective: To investigate male reproductive parameters via changes of potential testicular protein markers in restraint-stress rats. Methods: Male Sprague-Dawley rats were divided into two groups (non-immobilized control and restraint-immobilized/stress groups, n=8 each group). The stress animals were immobilized (12 h/d) by a restraint cage for 7 consecutive days. All reproductive parameters, morphology and histology were observed and compared between groups. In addition, the expression of steroidogenic acute regulatory (StAR) and phosphotyrosine proteins (previously localized in Sertoli and late spermatid cells) in testicular lysate was assayed by immuno-Western blotting. Results: Testosterone level, sperm concentration and sperm head normality of stress rats were significantly decreased while the corticosterone level was increased as compared with the control (P<0.05). Histologically, stress rats showed low sperm mass in epididymal lumen and some atrophy of seminiferous tubules. Although the expression of testicular StAR protein was not significantly different between groups, changed patterns of the 131, 95, and 75 kDa testicular phosphorylated proteins were observed in the stress group compared with the control group. The intensity of a testicular 95-kDa phosphorylated protein was significantly decreased in stress rats. Conclusions: This study has demonstrated the alteration of testicular phosphorylated protein patterns, associated with adverse male reproductive parameters in stress rats. It could be an explanation of some infertility in stress males.

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Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Changes of testicular phosphorylated proteins in response to restraint stress in male rats

Arun

Burawat

Sukhorum

et al. 2016

J. Zhejiang Univ. Sci. B

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“…[30][31][32] Before the SH, the sperm concentration was 24 to 167 × 10 6 /mL, the motility rate was 30% to 95%, and the proportion of morphologically normal sperm was 13% to 45%. Sperm concentrations, motility and normal morphology at pretreatment, treatment, and recovery phases are as shown in our previous study.…”

Section: Safety Of Sh For Subjectsmentioning

confidence: 99%

“…[30][31][32] As a continuation, this study is aimed to investigate the changes in sperm parameters, DNA, chromosome numbers, seminal biochemical substances (carnitine, EGF, and MIF), as well as reproductive hormones such as LH, FSH, and T in serum from 30 proven-fertile men subjected to SH at 43°C. [30][31][32] As a continuation, this study is aimed to investigate the changes in sperm parameters, DNA, chromosome numbers, seminal biochemical substances (carnitine, EGF, and MIF), as well as reproductive hormones such as LH, FSH, and T in serum from 30 proven-fertile men subjected to SH at 43°C.…”

Section: Introductionmentioning

confidence: 99%

Effect of scrotal heating on sperm quality, seminal biochemical substances, and reproductive hormones in human fertile men

Zhang

Zhai

Fang

et al. 2018

J of Cellular Biochemistry

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At present, male contraceptive methods are only vasectomy and condoms, so it is necessary to research on male contraceptive techniques. The aim of this study is to observe the effects of scrotal heating (SH) on semen parameters, seminal l-carnitine (LC), epidermal growth factor (EGF), macrophage migration inhibitory factor (MIF), reproductive hormones and sperm chromosome numbers of adult healthy men, and to provide the experimental data for male contraception. The scrotums of 30 healthy male volunteers were exposed to the condition of 40 to 43°C SH belt warming 40 minutes each day for successive 2 days per week. The course of SH was continuous for 3 months. Computer-assisted semen analysis and hypo-osmotic swelling test, sperm DNA integrity, l-carnitine, MIF and EGF, and sperm fluorescence in situ hybridization were performed before, during, and after SH. The serum level of follicle stimulating hormone (FSH), luteinizing hormone (LH), and testosterone (T) were measured by chemiluminescent immunoassay. The mean parameters of sperm concentration, vitality, and normal morphological sperm were significantly decreased in groups with sperms being collected during 1, 2, and 3 months of SH when compared with those in groups of pre-SH (P < 0.01). Statistically significant differences of sperm DNA fragmentation, normal sperm membrane functionality, levels of LC and MIF in semen, and LH, FSH, and T in serum were observed between the groups of before SH and after SH 3 months and the groups of during SH 1, 2, and 3 months (P < 0.001). The total rate of chromosome number for 13, 18, 21, X, and Y in the 3 months of SH was 13.7-fold greater (13.72%/1.69%) than before SH (P < 0.001). The constant SH can impact the semen quality, sperm DNA integrity, sperm chromosome, LC and MIF, and LH, FSH, and T in serum. Transient SH may be a new method for male contraception.

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“…The etiology of poor semen quality is not well understood, but given that spermatogenesis is a sensitive physiological process, environmental factors may play a key role [1]. Heat stress [10], diet [11], physical activity [12], psychosocial stress [13], and exposure to chemicals, such as bisphenol A [14], phthalates [15], pesticides [16], and metals [17], have been explored as potential determinants of semen quality.…”

Section: Introductionmentioning

confidence: 99%

Self-reported mobile phone use and semen parameters among men from a fertility clinic

Lewis

Mínguez-Alarcón

Meeker

et al. 2017

Reproductive Toxicology

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There is increasing concern that use of mobile phones, a source of low-level radio-frequency electromagnetic fields, may be associated with poor semen quality, but the epidemiologic evidence is limited and conflicting. The relationship between mobile phone use patterns and markers of semen quality was explored in a longitudinal cohort study of 153 men that attended an academic fertility clinic in Boston, Massachusetts. Information on mobile phone use duration, headset or earpiece use, and the body location in which the mobile phone was carried was ascertained via nurse-administered questionnaire. Semen samples (n=350) were collected and analyzed onsite. To account for multiple semen samples per man, linear mixed models with random intercepts were used to investigate the association between mobile phone use and semen parameters. Overall, there was no evidence for a relationship between mobile phone use and semen quality.

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Scrotal heat stress causes sperm chromatin damage and cysteinyl aspartate-spicific proteinases 3 changes in fertile men

Abstract: The continuously constant SHS can impact the semen quality, sperm DNA integrity, chromatin condensation and Caspase-3, and the combination of HOS plus AB test may simultaneously determine the integrity of membrane and chromatin condensation at the same spermatozoon.

Cited by 44 publications

References 55 publications

Changes of testicular phosphorylated proteins in response to restraint stress in male rats

Changes of testicular phosphorylated proteins in response to restraint stress in male rats

Effect of scrotal heating on sperm quality, seminal biochemical substances, and reproductive hormones in human fertile men

Self-reported mobile phone use and semen parameters among men from a fertility clinic

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