Changes of testicular phosphorylated proteins in response to restraint stress in male rats

Abstract

Abstract:Objective: To investigate male reproductive parameters via changes of potential testicular protein markers in restraint-stress rats. Methods: Male Sprague-Dawley rats were divided into two groups (non-immobilized control and restraint-immobilized/stress groups, n=8 each group). The stress animals were immobilized (12 h/d) by a restraint cage for 7 consecutive days. All reproductive parameters, morphology and histology were observed and compared between groups. In addition, the expression of steroidogenic acute regulatory (StAR) and phosphotyrosine proteins (previously localized in Sertoli and late spermatid cells) in testicular lysate was assayed by immuno-Western blotting. Results: Testosterone level, sperm concentration and sperm head normality of stress rats were significantly decreased while the corticosterone level was increased as compared with the control (P<0.05). Histologically, stress rats showed low sperm mass in epididymal lumen and some atrophy of seminiferous tubules. Although the expression of testicular StAR protein was not significantly different between groups, changed patterns of the 131, 95, and 75 kDa testicular phosphorylated proteins were observed in the stress group compared with the control group. The intensity of a testicular 95-kDa phosphorylated protein was significantly decreased in stress rats. Conclusions: This study has demonstrated the alteration of testicular phosphorylated protein patterns, associated with adverse male reproductive parameters in stress rats. It could be an explanation of some infertility in stress males.

Alterations of morphology and phosphorylated protein expression in the seminal vesicles of diabetic mice

“…Protein phosphorylation is a post-translational process of most cellular modifications including sperm physiological capacitation and acrosome exocytosis (Salicioni et al, 2007;Shivaji et al, 2007;Stival et al, 2016). Tyrosine-phosphorylated proteins are specifically localised within testicular tissue (Arad-Dann, Beller, Haimovitch, Gavrieli, & Ben-Sasson, 1993;Chaichun et al, 2017) and are assumed to play a role in spermatogenesis (Arun, Burawat, Sukhorum, Sampannang, Maneenin, et al, 2016;Arun, Burawat, Sukhorum, Sampannang, Uabundit, et al, 2016;Iamsaard et al, 2014;Sampannang et al, 2017Sampannang et al, , 2018 2013; Talat et al, 2016). Thus, monitoring for significant increases in OPN may help to predict microvascular diabetes complications (Talat et al, 2016).…”

“…Tyrosine protein phosphorylation is an important process in sperm capacitation and exocytosis of acrosome in natural fertilisation (Salicioni et al, 2007;Shivaji, Kumar, Mitra, & Jha, 2007;Stival et al., 2016). Since tyrosine-phosphorylated proteins are specifically present in Sertoli and late spermatic cells (Chaichun, Arun, Burawat, Kanla, & Iamsaard, 2017;Iamsaard et al, 2014), they are assumed to play important roles in spermatogenesis and androgen production (Arun, Burawat, Sukhorum, Sampannang, Maneenin, et al, 2016;Arun, Burawat, Sukhorum, Sampannang, Uabundit, et al, 2016;Iamsaard et al, 2014;Sampannang, Arun, Burawat, Sukhorum, & Iamsaard, 2018;Sampannang et al, 2017;Sawatpanich et al, 2018). Moreover, the expression of phosphorylated proteins in the lysate of testis and epididymis have been shown to change under adverse conditions, including stresses and cancer drug treatments, and these changes are associated with increases in endogenous acrosome reaction and sperm abnormality (Arun, Burawat, Sukhorum, Sampannang, Maneenin, et al, 2016;Arun, Burawat, Sukhorum, Sampannang, Uabundit, et al, 2016;Iamsaard et al, 2014;Sampannang et al, 2018Sampannang et al, , 2017Sawatpanich et al, 2018).…”

Valproic acid changes the expression of tyrosine‐phosphorylated proteins in rat seminal vesicle

“…The presence of TyrPho proteins in rat epididymal tissue and fluid has also been investigated (Sawatpanich et al, ), and improvements in testicular TyrPho protein changes have been associated with normal sperm and testosterone production (Burawat, Uabundit, Arun, Nualkaew, & Iamsaard, ; Iamsaard et al, , ; Iamsaard, Sukhorum, Sampannang, et al, ; Maneenin et al, ; Sampannang et al, ). Changes in TyrPho protein expression in the testis have been associated with stress‐related and diabetic conditions (Arun, Burawat, Sukhorum, Sampannang, Maneenin, et al, ; Arun, Burawat, Sukhorum, Sampannang, Uabundit, et al, ; Sampannang, Arun, Burawat, Sukhorum, & Iamsaard, ; Sampannang et al, ). Moreover, some anticancer drugs, such as VPA, methotrexate, ketoconazole and mimosine, have been shown to alter the patterns of TyrPho proteins in the testis and epididymis (Burawat et al, ; Iamsaard et al, , , ; Maneenin et al, ; Sawatpanich et al, ; Sukhorum & Iamsaard, ).…”

Distribution and differentiation of myeloid-derived suppressor cells after fluid resuscitation in mice with hemorrhagic shock

“…These pathophysiological changes may induce multiple organ dysfunction syndrome (MODS) and immune dysfunction, which are the most common causes of mortality in hemorrhagic shock Arun et al, 2016;Huber-Lang et al, 2016). Thus, the fundamental purposes of any investigation for hemorrhagic shock resuscitation should be not only improving microcirculatory flow, but also preventing MODS and immune dysfunction.…”