Screw-Capped Bottles in the Preparation and Storage of Culture Media

The Maintenance of Aerobic, Micro-Aerophilic and Anaerobic Conditions in a Petri Dish

“…The increasing use of screw-cap medicine bottles for the storage of bacteriological media (McCartney, 1933) makes generally available the described routine method for the preparation of agar media which are themselves a source of the carbon dioxide necessary for the growth of many organisms. Directions for the preparation of sterile solutions containing sodium bicarbonate usually call for a filtration method of sterilization (Parker, 1938).…”

Blood Transfusion with Rotary Pump

“…Another disadvantage of the all-metal syringe is that the blood cannot be seen during Positive air pressure as a means of introducing blood was employed by Robertson towards the end of the great war. " Robertson's bottle " was later improved by Keynes (1920), and this apparatus has in turn been modified by McCartney (1933). The principle of these methods is to create a positive pressure in the flask or bottle used by means of bellows attached to a side-arm.…”

“…On one occasion in my experience, when the bung was produced after a long interval, it could not be used on account of the stiffening. McCartney (1933) has overcome this particular difficulty by substituting a metal screw cap carrying an inlet and outlet tube in the place of the rubber bung. This has the advantage that it is a standard fitting for the screw-cap bottle described later, and so will last indefinitely.…”

A simple tellurite-chocolate-agar medium for the typing and isolation of Corynebacterium diphtheriae

“…at 900 C. Filter and bring to pH 7 6 with N/l HCI. Bottle in screw-top bottles as described by McCartney (1933).…”