Methods recommended for the coagulation and sterilization of blood serum or egg media by the use of steam under pressure are of two types. In the first, all, or some indefinite part, of the air originally present is retained in the autoclave by keeping all valves closed except the steam inlet during both coagulation and sterilization. This results in temperatures below the maximum attainable for the operating pressure and in variations of temperature in different parts of the autoclave due to stratification of the steam; the indefinite temperature established makes prolonged treatment necessary; and, in the case of autoclaves which have their thermometers in the exhaust, the temperature cannot be ascertained.
A MANOMETRIC VALVE OR RESPIRATOR THIS valve permits of a fully automatic increase and decrease in pressure, over a variable fixed range, in reaction or culture vessels or rooms; and it obviates the discharge of, and consequent replacement of, waste liquid from the valve. Mercury is added to the sepa-
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