Screening to Identify Commonly Used Chinese Herbs That Affect <i>ERBB2</i> and <i>ESR1</i> Gene Expression Using the Human Breast Cancer MCF‐7 Cell Line

Chiu, Jen‐Hwey; Chang, Chun Ju; Wu, Jing; Liu, Hui Ju; Wen, Che Sheng; Hsu, Cheng Hsiung; Chen, Jiun Liang; Tseng, Ling Ming; Chen, Wei Shone; Shyr, Yi Ming

doi:10.1155/2014/965486

Cited by 11 publications

(9 citation statements)

References 34 publications

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“…This aspect is specifically strengthened by the data that extracts from herbs selectively efficacious for the ER-F or ER-NF phenotype function via distinct effects on cell cycle progression, cellular apoptosis and formation of anti-proliferative E2 metabolites. Furthermore, the models developed and the results obtained in the present study provide relevant significance to the herbal management of ER + or ER -clinical breast cancer, as well to the relapse of ER + breast cancer, in which the ER expression has become negative (4,5,26,27,30). Clearly, clinical usefulness of the nutritional herbs investigated in the present study will have to be established in future human studies.…”

Section: Er-nf Er-f -------------------------------------------------mentioning

confidence: 81%

“…It is noteworthy that human breast carcinoma-derived cell culture models with differing expression of ER, PR, HER-2, epidermal growth factor receptor, p53 and retinoblastoma protein have been utilized to examine the growth-inhibitory effects of aqueous extracts prepared from several Chinese medicinal herbs (26,27). However, it should be recognized that intrinsic genetic differences in expression of hormone and growth factor receptors in individual cell lines preclude justifiable comparative investigations within the existing models.…”

Section: Er-nf Er-f -------------------------------------------------mentioning

confidence: 99%

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Inhibitory effects of Chinese nutritional herbs in isogenic breast carcinoma cells with modulated estrogen receptor function

Telang¹,

Guo²,

Katdare

et al. 2016

Oncology Letters

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Abstract. In estrogen receptor (ER)+ MCF-7 cells, ER represents a ligand-activated transcription factor, and 17β-estradiol (E2) represents its physiological ligand. Maintenance of the human breast carcinoma-derived MCF-7 cells with 0.7% serum selected a proliferative sub-population of E2-responsive cells with transiently non-functional ER due to limited availability of E2. Culture of MCF-7 cells in the presence of either 0.7% serum, <1 nM E2 or 0.7% serum + 20 nM E2 selected isogenic cells with either

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Section: Er-nf Er-f -------------------------------------------------mentioning

confidence: 81%

Section: Er-nf Er-f -------------------------------------------------mentioning

confidence: 99%

Inhibitory effects of Chinese nutritional herbs in isogenic breast carcinoma cells with modulated estrogen receptor function

Telang¹,

Guo²,

Katdare

et al. 2016

Oncology Letters

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“…Traditionally, herbs are boiled in water and consumed by patients as herbal tea (7,8,(23)(24)(25). Therefore, to simulate patient consumption, non-fractionated aqueous extracts from EG were used as the test agent in the present study.…”

Section: Discussionmentioning

confidence: 99%

The nutritional herb Epimedium grandiflorum inhibits the growth in a model for the Luminal A molecular subtype of breast cancer

Telang¹,

Gou²,

Katdare

et al. 2017

Oncology Letters

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Abstract. The Luminal A subtype of breast cancer expresses the estrogen receptor (ER)-α and progesterone receptor (PR), but not the human epidermal growth factor receptor (HER)-2 oncogene. This subtype of breast cancer responds to endocrine therapy involving the use of selective estrogen receptor modulators and/or inhibitors of estrogen biosynthesis. However, these therapeutic agents are frequently associated with long-term systemic toxicity and acquired tumor resistance, emphasizing the need to identify non-toxic alternative treatments for chemo-endocrine therapy responsive breast cancer. The present study utilized the human mammary carcinoma-derived, ER + /PR + /HER-2 -MCF-7 cell line as a model of the Luminal A subtype of breast cancer to examine the growth inhibitory effect of the Chinese nutritional herb Epimedium grandiflorum (EG) and determine the mechanisms underlying this effect. MCF-7 cells maintained in a serum-depleted culture medium retained their ability to grow in response to 17β-estradiol (E 2 ). Treatment of the MCF-7 cells with EG resulted in dose-dependent inhibition of E 2 -promoted growth. Mechanistically, EG inhibited E 2 -promoted cell cycle progression through G 1 stage arrest and modulated the cellular metabolism of E 2 , increasing the formation of the anti-proliferative metabolites 2-hydroxyestrone and estriol. Long-term treatment of MCF-7 cells with EG inhibited E 2 -promoted anchorage independent growth, a surrogate in vitro biomarker of tumorigenesis. In conclusion, the results of the present study demonstrate the growth inhibitory effects of EG on MCF-7 cells and identified clinically relevant mechanistic leads for its anti-tumorigenic efficacy. IntroductionMetastatic breast cancer is one of the leading causes of breast-cancer associated mortality in the United States, and the American Cancer Society (Atlanta, GA, USA) projections for breast cancer incidence and mortality rates estimate 246,660 newly diagnosed cases of invasive breast cancer and 40,450 metastatic breast cancer-associated mortalities in women in 2017 (1). These figures emphasize the requirement for a more precise molecular classification of breast cancer for subtype-specific targeted therapy, in addition to the necessity to identify novel, non-toxic and efficacious modalities as alternatives for cancer prevention and therapy.The global gene expression profiling of breast cancer has led to the identification of molecularly distinct subtypes. Patients with the molecular subtype Luminal A express estrogen receptor-α (ER)-α and progesterone receptor (PR), and they lack the expression of human epidermal growth factor receptor-2 (HER-2). Patients with this subtype respond to endocrine-based targeted therapy, such as selective ER modulators and specific small molecule inhibitors of estrogen biosynthesis (2-4). However, long-term endocrine-based therapy has been associated with adverse systemic toxicity, acquired tumor resistance and the emergence of drug resistant cancer stem cells that compromise therapeutic efficacy and ...

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“…The voucher specimens were prepared as water extracts of the CHEs by the company, which followed the standard GMP procedures in order to obtain compositions similar to those used clinically. The contents of the CHEs were standardized using high performance liquid chromatography (Additional file 1 ) [ 18 ]. To decrease any confounding effect caused by the presence of lipopolysaccharide contamination during the herbal preparation, polymyxin B (10 μg/mL) was administration routinely during each experiment.…”

Section: Methodsmentioning

confidence: 99%

“…Unfortunately, many patients with breast cancer who use integrative therapies are not aware of the potential adverse effects of these additional treatments. For examples, the consumption of many Chinese herbal extracts can have an up-regulatory effect on the gene expression levels of ER and HER2 in vitro [ 18 ] as well as interacting with the effects of tamoxifen in vivo [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Chinese medicinal herbs on expression of brain-derived Neurotrophic factor (BDNF) and its interaction with human breast cancer MDA-MB-231 cells and endothelial HUVECs

Chiu¹,

Chen²,

Tsai³

et al. 2017

BMC Complement Altern Med

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BackgroundOur previous study demonstrated that an up-regulation of the Brain-Derived Neurotrophic Factor (BDNF) signaling pathway is involved the mechanism causing the recurrence of triple negative breast cancer. The aim of this study is to investigate the effects of commonly used Chinese medicinal herbs on MDA-MB-231 and HUVEC cells and how they interact with BDNF.MethodsHuman TNBC MDA-MB-231 cells and human endothelial HUVEC cells were used to explore the effect of commonly used Chinese herbal medicines on cancer cells alone, on endothelial cells alone and on cancer cell/endothelial cell interactions; this was done via functional studies, including migration and invasion assays. Furthermore, Western blot analysis and real-time PCR investigations were also used to investigate migration signal transduction, invasion signal transduction, and angiogenic signal transduction in these systems. Finally, the effect of the Chinese medicinal herbs on cancer cell/endothelial cell interactions was assessed using co-culture and ELISA.ResultsIn terms of autoregulation, BDNF up-regulated TrkB gene expression in both MDA-MB-231 and HUVEC cells. Furthermore, BDNF enhanced migration by MDA-MB-231 cells via Rac, Cdc42 and MMP, while also increasing the migration of HUVEC cells via MMP and COX-2 expression. As measured by ELISA, the Chinese herbal medicinal herbs A. membranaceus, P. lactiflora, L. chuanxiong, P. suffruticosa and L. lucidum increased BDNF secretion by MDA-MB-231 cells. Similarly, using a co-culture system with MDA-MB-231 cells, A. membranaceus and L. lucidum modulated BDNF-TrkB signaling by HUVEC cells.ConclusionWe conclude that BDNF plays an important role in the metastatic interaction between MDA-MB-231 and HUVEC cells. Some Chinese medicinal herbs are able to enhance the BDNF-related metastatic potential of the interaction between cancer cells and endothelial cells. These findings provide important information that should help with the development of integrated medical therapies for breast cancer patients.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-017-1909-7) contains supplementary material, which is available to authorized users.

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Screening to Identify Commonly Used Chinese Herbs That Affect ERBB2 and ESR1 Gene Expression Using the Human Breast Cancer MCF‐7 Cell Line

Cited by 11 publications

References 34 publications

Inhibitory effects of Chinese nutritional herbs in isogenic breast carcinoma cells with modulated estrogen receptor function

Inhibitory effects of Chinese nutritional herbs in isogenic breast carcinoma cells with modulated estrogen receptor function

The nutritional herb Epimedium grandiflorum inhibits the growth in a model for the Luminal A molecular subtype of breast cancer

Effects of Chinese medicinal herbs on expression of brain-derived Neurotrophic factor (BDNF) and its interaction with human breast cancer MDA-MB-231 cells and endothelial HUVECs

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