Screening Study on Antioxidant Activity of Plants Grown Wildly in Mongolia

Enkhtuya, Enkhtsetseg; Kashiwagi, Takanari; Shimamura, Takeshi; Ukeda, Hiroyuki; Odbayar, Tseye-Oidov

doi:10.3136/fstr.20.891

Cited by 10 publications

(10 citation statements)

References 30 publications

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“…Enkhtuya et al, investigated radical scavenging and reducing activities of different plants, and found that in DPPH, ABTS, and FRAP methods of analysis lingonberry leaves were more active than leaves of black currant, hawthorn, strawberry, and tall currant. Radical scavenging and reducing properties of lingonberry leaves were similar to that of seabuckthorn [ 16 ]. Vyas et al found significant differences of radical scavenging and reducing activities of lingonberry leaves within wild clones and cultivars, and distinguished V. vitis-idaea subsp.…”

Section: Discussionmentioning

confidence: 99%

“…This indicates that lingonberry leaves exhibit great ability to donate hydrogen atom or transfer single–electron. Concerning chelating activity, some researchers reported that lingonberry extracts showed weaker chelating activity than extracts from plants belonging to other families [ 16 , 17 ]. However, it is difficult to compare the antioxidant activity results obtained in the present study with the literature data, because of the differences in methods and expression of antioxidant activity.…”

Section: Discussionmentioning

confidence: 99%

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Antioxidant Activities of Vaccinium vitis-idaea L. Leaves within Cultivars and Their Phenolic Compounds

et al. 2019

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Lingonberry leaves are the subject of numerous studies because of antioxidant properties, positive influence on the health and potential use in the prevention and treatment of chronic diseases. In this work, the radical scavenging, reducing, chelating activities, and phenolic composition of ten lingonberry leaves cultivars, one subspecies, and one variety were investigated. Furthermore, the antioxidant activity of individual phenolic compounds, that can be found in lingonberry leaves, were analyzed, and structure-activity relationship was determined. Wide diversity for phenolic profile and antioxidant properties of lingonberry leaves has been observed in the present material. Cultivars ‘Kostromskaja rozovaja’, ‘Rubin’, and Vaccinium vitis-idaea var. leucocarpum surpassed all others tested cultivars and lower taxa by contents of phenolic compounds and antioxidant activity. Leaves of lingonberry cultivars and lower taxa are rich in arbutin, flavonol glycosides, proanthocyanidins, and the latter were considered to be the major contributor to antioxidant properties of lingonberry leaves.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Antioxidant Activities of Vaccinium vitis-idaea L. Leaves within Cultivars and Their Phenolic Compounds

et al. 2019

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“…According to our previous results, the 50% ethanol extract of peony leaf showed remarkable scavenging activity against DPPH radicals, ABTS radical cations and superoxide anions, and exerted significant ferric reducing antioxidant power. The extract also contained a considerable amount of phenolics (Enkhtuya et al, 2014).…”

Section: Resultsmentioning

confidence: 98%

“…We previously reported a screening study on the antioxidant activity of leaves from eight plants growing wild in Mongolia (Enkhtuya et al, 2014). From the results, we found that peony leaf was the most effective candidate for further study.…”

Section: Introductionmentioning

confidence: 94%

Antioxidative Constituents in the Leaves of <i>Paeonia anomala</i> Grown in Mongolia

Enkhtuya

Shimamura

Kashiwagi

et al. 2017

FSTR

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We successfully identified the main constituents responsible for the high antioxidant properties of the leaves ofPaeonia anomala (Paeoniaceae) for the first time. P. anomala is an endemic plant widely found throughout Mongolia, and its air-dried leaves are main ingredients in some kinds of herbal teas. Infusions of P. anomala leaves are also used as home remedies for the treatment of liver and kidney ailments by the local population. Three major antioxidative constituents were isolated from the ethyl acetate layer of a P. anomala leaf ethanol extract via 1,1-diphenyl-2picrylhydrazyl (DPPH) assay-guided fractionation, consisting of liquid-liquid partition, two types of open column chromatography and reversed-phase HPLC. Chemical structures of the purified compounds were identified using LC-MS, 1 H-NMR, and 13 C-NMR as methyl gallate, pentagalloylglucose, and tellimoside. The identified compounds contributed to 35% of the total DPPH radical scavenging activity of the P. anomala leaf ethanol extract.Preparation of crude extract To isolate and identify the antioxidative constituents, powdered peony leaf (50 g) was extracted with 50% (v/v) ethanol/water (1000 mL) with constant mixing by magnetic stirrer for 2 h at room temperature. The suspension was centrifuged using a refrigerated centrifuge (18PR-52; Hitachi) at 6,800 × g for 20 min at 4℃, and the supernatant was stocked. This procedure was repeated twice and the supernatants were pooled, vacuum filtered through No. 5C filter paper (Advantec Toyo, Tokyo, Japan), evaporated under reduced pressure at 37℃ to remove the solvent, and then freeze-dried. DPPH radical scavenging assay The scavenging activity of samples, including the peony leaf ethanol extract, its fractions, and reference compounds, against DPPH radicals was determined as described by Adedapo et al. (2009) with minor modifications. A 2-mL aliquot of 0.135 mM DPPH (Wako Pure Chemical Industries, Osaka, Japan) in methanol was mixed with 100 μL of sample diluted with methanol. After 30 min in the dark at room temperature, absorbance of the reaction mixture was measured at 517 nm. The DPPH radical solution was freshly prepared daily and kept in the dark during measurements. Results were reported as IC 50 values, which is the amount of antioxidant necessary to scavenge the initial DPPH radical concentration by 50%. To investigate the IC 50 values, the dose-response relationship was studied and the capacity to scavenge DPPH radicals was expressed as a percentage according to the following equation: DPPH scavenging capacity (%) = (1 _ Absorbance of sample at 517 nm Absorbance of control at 517 nm ) ·100 ······Eq. 1 Known standard antioxidants, namely L (+)-ascorbic acid (Wako Pure Chemical Industries), quercetin hydrate (Tokyo Chemical Industry, Tokyo, Japan), butylated hydroxytoluene (BHT, Nacalai T e s q u e , K y o t o , J a p a n ) , a n d 6 -h y d r o x y -2 , 5 , 7 , 8tetramethylchroman-2-carboxylic acid (Trolox, Sigma-Aldrich, St.Louis, MO), were used as reference compounds. Isolation of antioxidative com...

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“…Among the selected plant leaves, V.vitis-idaeae, P.anomala, R.acicularis, and H.rhamnoides had significant antioxidant potential and were rich in polyphenolics. In particular, P.anomala leaf showed remarkable scavenging activity against DPPH radicals, ABTS radical cations and superoxide anions, and exerted significant ferric reducing antioxidant power [1]. Afterwards, we identified three main constituents in P.anomala leaf, namely methyl gallate, pentagalloylglucose, and tellimoside, which showed potent DPPH radical scavenging activity [2].…”

Section: Introductionmentioning

confidence: 99%

Screening Study on Enzyme Inhibitory Activities of Some Mongolian Plant Leaves

Enkhtuya¹,

Shimamura²,

Kashiwagi³

et al. 2018

JFNR

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In this study, 50% ethanol extract prepared from air-dried leaves of nine plants grown wildly in Mongolia were characterized for their inhibitory activity of tyrosinase, α-glucosidase, hyaluronidase, lipase, and angiotensin I-converting enzyme (ACE) in order to identify potential new sources for dietary prevention of certain non-communicable diseases and skin health promotion from domestic plant kingdom. The results of this study indicate that out of the selected nine Mongolian plant leaves, V.vitis-idaeae, R.altissimum, and R.acicularis leaves presented strong inhibitory activity of mushroom tyrosinase, R.acicularis and P.anomala leaves exhibited good anti α-glucosidase and anti hyaluronidase activity, V.vitis-idaeae leaf showed appreciable inhibition of lipase activity, and P.anomala leaf effectively inhibited ACE. Among them P.anomala and R.acicularis leaves were found to be the most promising natural source with multiple biological properties.

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Screening Study on Antioxidant Activity of Plants Grown Wildly in Mongolia

Cited by 10 publications

References 30 publications

Antioxidant Activities of Vaccinium vitis-idaea L. Leaves within Cultivars and Their Phenolic Compounds

Antioxidant Activities of Vaccinium vitis-idaea L. Leaves within Cultivars and Their Phenolic Compounds

Antioxidative Constituents in the Leaves of <i>Paeonia anomala</i> Grown in Mongolia

Screening Study on Enzyme Inhibitory Activities of Some Mongolian Plant Leaves

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