Screening Spanish isolates of steinernematid nematodes for use as biological control agents through laboratory and greenhouse microcosm studies

Campos‐Herrera, Raquel; Gutiérrez, Carmen

doi:10.1016/j.jip.2008.11.009

Cited by 46 publications

(31 citation statements)

References 27 publications

(28 reference statements)

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“…). However, research on susceptibility of wireworms to entomopathogenic nematodes has been limited and produced inconsistent results (Eidt and Thurston ; Campos‐Herrera and Gutierrez ; Půža and Mráček ). Studies with A. lineatus larvae showed no susceptibility to Steinernema feltiae (Filipjev) and mortality between 17% and 67% with various Heterorhabditis bacteriophora Poinar strains (Ansari et al.…”

Section: Introductionmentioning

confidence: 99%

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Laboratory and field evaluation of entomopathogenic nematodes for control of Agriotes obscurus (L.) (Coleoptera: Elateridae)

Morton

Pino

2016

J Applied Entomology

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The susceptibility of the dusky wireworm, Agriotes obscurus (L.) (Coleoptera: Elateridae), to different species and strains of entomopathogenic nematodes was tested in a virulence assay in the laboratory. Larvae were exposed to different nematode doses of 50 and 100 IJs/cm2. At a dose of 50 IJs/cm2, only a commercial strain Heterorhabditis bacteriophora Poinar and the native strain Steinernema carpocapsae (Weiser) B14 caused increased mortality compared with the control (11.1% and 13.3% mortality, respectively). At the higher dose tested, all strains (except Steinernema sp. D122) were virulent to A. obscurus larvae. Steinernema carpocapsae B14 caused higher mortality of wireworm (75.6%) and was used for the assay conducted in cages, with a dose of 100 IJs/cm2, in field conditions. The results showed that S. carpocapsae B14 controlled 48.3% of A. obscurus larvae, demonstrating that some entomopathogenic nematodes have the potential to control larvae of A. obscurus. However, further work is needed to improve their efficacy.

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Section: Introductionmentioning

confidence: 99%

“…). However, most of the research on wireworm control with EPNs did not specify the insect species nor the larval stage of the wireworm tested (Campos‐Herrera and Gutierrez ; Půža and Mráček ; San‐Blas et al. ).…”

Section: Introductionmentioning

confidence: 99%

Laboratory and field evaluation of entomopathogenic nematodes for control of Agriotes obscurus (L.) (Coleoptera: Elateridae)

Morton

Pino

2016

J Applied Entomology

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“…Soil porosity was found to be positively associated with EPN predation rates in a number of studies (Barbercheck, 1992;Barbercheck and Kaya, 1991;Campos-Herrera and Gutiérrez, 2009;Hara et al, 1991;Hazir et al, 2003;Kaspi et al, 2010;Kung et al, 1990;Liu and Berry, 1995;McCoy et al, 2003;Portillo-Aguilar et al, 1999;Rueda et al, 1993;Stuart et al, 2006;Zhang et al, 1992), and predation of DRW by EPNs during 2 years was much greater in a central ridge orchard on coarse sand than in a flatwoods orchard on finer textured, sandy loam soil (Duncan et al, 2003). In contrast, Shapiro et al (2000) demonstrated greater efficacy by two EPN species against DRW in fine textured compared to coarse textured soils at a standard water potential and speculated that greater water content might have favored EPNs in the finer soil.…”

Section: Introductionmentioning

confidence: 99%

Entomopathogenic nematodes, root weevil larvae, and dynamic interactions among soil texture, plant growth, herbivory, and predation

El-Borai

Stuart

Campos‐Herrera

et al. 2012

Journal of Invertebrate Pathology

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Greenhouse experiments were conducted to assess the influence of soil texture on the persistence, efficacy and plant protection ability of entomopathogenic nematodes (EPNs) applied to control larvae of the Diaprepes root weevil (DRW), Diaprepes abbreviatus, infesting potted citrus seedlings. Seedlings were grown in pots containing either coarse sand, fine sand, or sandy loam. Three DRW larvae were added to each of 80 pots of each soil type. 24 h later, 20 pots of each soil type that had received weevil larvae were inoculated with EPN infective juveniles (IJs) of one of the following species: Steinernema diaprepesi, Steinernema riobrave and Heterorhabditis indica. Pots of each soil without EPNs were established as controls with DRW and controls without DRWs. Subsequently, pots with larvae received three additional larvae monthly, and the experiment continued for 9 months. Plant root and top weights at the end of the experiment were affected by both soil (P≤0.0001) and nematodes (P≤0.0001), and nematode species protected plants differently in different soils (interaction P≤0.0001). Soil porosity was inversely related to plant damage by DRW, whether or not EPNs were present; and porosity was directly related to the level of plant protection by EPNs. Mortality of caged sentinel weevil larvae placed in pots near the end of the experiment was highest in pots treated with S. diaprepesi. In a second, similar experiment that included an additional undescribed steinernematid of the Steinernema glaseri-group, soil type affected root damage by DRW and root protection by EPNs in the same manner as in the first experiment. Final numbers of S. diaprepesi and Steinernema sp. as measured by real-time PCR were much greater than those of S. riobrave or H. indica in all soils. Across all treatments, the number of weevil larvae in soil at the end the experiment was inversely related to soil porosity. In all soils, fewer weevil larvae survived in soil treated with S. diaprepesi or Steinernema sp. than in controls with DRW or treatments with S. riobrave or H. indica. The results of these experiments support the hypothesis that EPNs provide greater protection of seedlings against DRW larvae in coarse textured soil than in finer textured soil. However, less vigorous growth of the control without DRW seedlings in the two finer textured soils suggests that unidentified factors that stressed seedlings in those soils also impaired the ability of seedlings to tolerate weevil herbivory.

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“…Elaterid larvae are generally considered to be resistant, or have very little susceptibility to EPN infection (Eidt and Thurston, 1995;Campos-Herrera and Gutierrez, 2009). Eidt and Thurston (1995) stated that elaterid larvae possess several physical deterrents (dense hairs in the mouth opening, thick cuticle, valvate spiracles, muscles closing anus) that protect them from EPN invasion.…”

Section: Discussionmentioning

confidence: 99%

Does scavenging extend the host range of entomopathogenic nematodes (Nematoda: Steinernematidae)?

Půža

Mráček

2010

Journal of Invertebrate Pathology

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Screening Spanish isolates of steinernematid nematodes for use as biological control agents through laboratory and greenhouse microcosm studies

Cited by 46 publications

References 27 publications

Laboratory and field evaluation of entomopathogenic nematodes for control of Agriotes obscurus (L.) (Coleoptera: Elateridae)

Laboratory and field evaluation of entomopathogenic nematodes for control of Agriotes obscurus (L.) (Coleoptera: Elateridae)

Entomopathogenic nematodes, root weevil larvae, and dynamic interactions among soil texture, plant growth, herbivory, and predation

Does scavenging extend the host range of entomopathogenic nematodes (Nematoda: Steinernematidae)?

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