Screening, Purification and Characterization of Anionic Antimicrobial Proteins from Foeniculum Vulgare

Abstract

Foeniculum vulgare Mill., commonly called fennel, is a medicinal plant belonging to the Umbelliferae (Apiaceae) family, and is used in traditional medicine. Antibacterial peptides were isolated using sodium phosphate citrate buffer and, for extraction, cetyltrimethyl ammonium bromide (CTAB) buffer with pH 6, have been employed and antimicrobial activity tested against four reference strains. The extracted protein was subjected to 3 kDa dialysis and separation was carried out by DEAE-ion exchange chromatography and further proteins were identified by 2D gel electrophoresis. The results of Foeniculum vulgare elutes obtained from DEAE-ion exchange chromatography were tested for antibacterial activity. Elute 3 shows the highest antibacterial activity on Pseudomonas aeruginosa with a diameter of a zone of inhibition of 16 mm and IC50 value 25.02 (mcg/mL). Based on the findings of the wide usage in treatment of various ailments and day-to-day life, Foeniculum vulgare seeds were used in the present research and have shown promising antibacterial effects, which requires further proteomic research to authenticate the role of the anticipated proteins.

Peptide-Based Drugs and Drug Delivery Systems

“…New insight into the identification and characterization of novel AMPs is reported by Al Akeel et al [ 3 ]. New promising anionic antimicrobial compounds were derived from Foeniculum vulgare , a therapeutic plant belonging to the Umbelliferae (Apiaceae) family, which is widely used in traditional chinese medicine.…”

Screening & analysis of anionic peptides from Foeniculum vulgare Mill by mass spectroscopy

“…We previously reported on the antimicrobial activity of anion exchange chromatography purified seed extracts from F. vulgare seed (Al Akeel et al, 2017). Preliminary examination of the active fractions suggested that one particularly potent elute fraction (# 2) was relatively non-complex with respect to the number of proteins involved and therefore appeared to be a good candidate for characterization.…”

“…Convectional protocol is used as per the manual (Agilent technologies). In brief, MS grade trypsin used in gel digestion to achieve maximum specific proteins from the gel and the procedures goes as per our previous study (Al Akeel et al, 2017).…”

Purification and MIC analysis of antimicrobial proteins from Cucumis sativus L. seeds

“…The extraction and isolation procedures were as per our previous study where we have standardized our protocols for this project [ 15 ]. In brief, seed extraction was initiated by first cleaning the seeds with tap water and then distilled water to remove dirt or debris.…”