Screening of Tropical Medicinal Plants for Molluscicidal, Larvicidal, Fungicidal and Cytotoxic Activities and Brine Shrimp Toxicity

Biological activity of neosergeolide and isobrucein B (and two semi-synthetic derivatives) isolated from the Amazonian medicinal plant Picrolemma sprucei (Simaroubaceae)

“…Namely, the signal at δ 5.11 in the The brine shrimp assay is a simple, inexpensive method which allows for the evaluation of general toxicity and is considered a preliminary assay in the study of extracts and secondary metabolites for cytotoxic and antitumor activity. LC 50 values in the brine shrimp assay correlate well with other in vitro tests such as those for antitumor activity, cytotoxicity (Meyer et al 1982, Munro et al 1987, Siqueira et al 2001) and antineoplastic activity (Badaway & Kappe 1997) and also for in vivo toxicity (LD 50 ) of extracts administered by gavage in Swiss albino mice (Parra et al 2001) and pesticidal (Fatope et al 1993), larvicidal, fungicidal, molluscicidal (Cepleanu et al 1994), antimalarial (Pérez et al 1997), insecticidal (Oberlies et al 1998) and antifeedant activities (Labbe et al 1993). Quassinoids 1 and 2 were considered very toxic toward A. franciscana, while 3 showed low toxicity (Fig.…”

The good and the bad: Alkaloid screening and brineshrimp bioassays of aqueous extracts of 31 medicinal plants of eastern Nicaragua

“…The brine shrimp lethality assay (BSLA) was used to determine if the plant extracts of medicinal species were cytotoxic (Meyer et al, 1982;McLaughlin et al, 1991;Cepleanu et al, 1994;Coe et al, 2010). Ten brine shrimp larvae were placed in each of the triplicate vials (thus, 30 shrimp per concentration) using a plastic pipette with a 2 mm diameter tip.…”

Constituintes químicos do caule de Spathelia excelsa (rutaceae) e atividade frente a Aedes aegypti

“…Os ensaios foram feitos em triplicata e a leitura realizada após 24 h, verificando-se o número de larvas mortas. 21 Os dados obtidos foram lançados em gráfico de probitos da mortalidade x concentração através de programa estatístico StatsDirect 2.6.6. StatsDirect Limited para determinação das concentrações letais.…”