Screening of some medicinal plants of Ethiopia for their anti-microbial properties and chemical profiles

Geyid, Aberra; Abebe, Dawit; Debella, Asfaw; Makonnen, Zewdneh; Aberra, Frehiwot; Teka, Frehiwot; Kebede, Tesfaye; Urga, Kelbessa; Yersaw, Kidist; Biza, Teklele; Mariam, Bisrat Haile; Guta, Mulugeta

doi:10.1016/j.jep.2004.08.021

Cited by 195 publications

(156 citation statements)

References 9 publications

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“…Similarly, antifungal effect of A. mexicana was previously reported by [13]. The antifungal activity of methanolic extract of D. abyssinica has also been reported [14].…”

Section: Mycelial Inhibition Testsupporting

confidence: 65%

Antifungal Activity of Some Plant Extracts against (Colletotrichum Musae) the Cause of Postharvest Banana Anthracnose

Bazie¹,

Ayalew²,

Woldetsadik³

2014

J Plant Pathol Microbiol

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Materials and Methods Experimental materialsFresh leaves of 21 plant species were collected from local sources. The identity of these plant species was confirmed at Haramaya University Herbarium where voucher specimens are deposited. The plants included Acacia albida, A. seyal Isolation of the pathogen and inoculum preparationBanana fruits showing anthracnose disease symptoms were collected and used for isolation of Colletotrichum musae. Pure culture was maintained on PDA medium. Inoculum was prepared by dislodging the surface of 10 days old culture with sterile distilled water and adjusted to 105 ml -1 using a hemacytometer. Extraction of plant samplesExtraction was done using methanol and water. The methanolic extracts were used for preliminary screening of the plant specimens for their potency against the fungal pathogen, while aqueous extracts were used for further testing. Fifty gram of powdered samples of each of the plant specimens was extracted with 250 ml solvent (methanol or water). The methanolic and aqueous extracts were filtered and reduced to dryness on rotary evaporator under vacuum at 40°C and 60°C water bath temperatures, respectively [8,9]. The extract of each plant was weighed, re-dissolved and then tested for antifungal activities. In vitro assay of plant extractsPaper disc method: The method as described by [9] was used. AbstractThe present study was conducted to determine the efficacy of extracts of some plant species against Colletotrichum musae. Methanolic extracts of 21 plant species were screened for their inhibitory effect against C. musae using paper disc method and spore germination assay. Among them, extract of Prosopis juliflora exhibited superior antifungal activity (30.7 mm), followed by Acacia albida (19 mm) as compared to nill in the control. On the other hand, carbendazim, which was used as a standard chemical check, demonstrated by far the highest inhibition zone of 51.7 mm diameter. Extracts from A. albida, Dovalis abyssinica and P. juliflora reduced conidial germination to 0.2, 0.5 and 0.3%, respectively, which didn't vary statistically from 1.2% in Carbendazim. Six plant species, viz., A. albida, Azadirachta indica, Argemone mexicana, D. abyssinica, P. juliflora and Vernonia amygdalina, that showed high to moderate antifungal activity in the preliminary screening, were further tested for their thermal stability at 60°C and for the efficacy of their aqueous extracts against C. musae. Extracts of the tested plant species were found to be heat stable and aqueous extracts of A. albida showed the highest antifungal activity (18 mm), followed by P. juliflora (12.3 mm). Further studies need to be undertaken to isolate the active compounds from those extracts with fungicidal potential.

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“…Similarly, antifungal effect of A. mexicana was previously reported by [13]. The antifungal activity of methanolic extract of D. abyssinica has also been reported [14].…”

Section: Mycelial Inhibition Testsupporting

confidence: 65%

Antifungal Activity of Some Plant Extracts against (Colletotrichum Musae) the Cause of Postharvest Banana Anthracnose

Bazie¹,

Ayalew²,

Woldetsadik³

2014

J Plant Pathol Microbiol

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“…The antimicrobial activity of the extracts of the various parts of the investigated plants such as roots, leaves, seeds, stem barks and fruits, appears to be due to the presence of secondary metabolites such polyphenols, triterpenes, sterols, saponins, tannins, alkaloids, glycosides and polysaccharides (Geyid et al, 2005;James et al, 2007;Maikai et al,2009;Ogunleye et al, 2003).…”

Section: Antimicrobial and Antifungal Activitiesmentioning

confidence: 99%

Ximenia americana: Chemistry, Pharmacology and Biological Properties, a Review

Monte¹,

Lemos²,

Araújo³

et al. 2012

Phytochemicals - A Global Perspective of Their Role in Nutrition and Health

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“…Traditionally stem bark decoction of A. gummifera is used to treat malaria and an extract from fresh crushed pods is used to treat stomach pains. The seeds and stem bark of the plants have shown antibacterial activities against both gram positive and gram negative bacteria [4], molluscidal activities against Biomphalaria pfeifferi, Bulinus sp. and physaacuta [5], larvicidal activity against Aedes agypti, Aedes africanus, and Culex…”

Section: Introductionmentioning

confidence: 99%

Evaluations of Biochemical, Hematological and Histopathological Parameters of Subchronic Administration of Ethanol Extract of Albizia Gummifera Seed in Albino Wistar Rat

Debebe¹,

Afework²,

Makonnen³

et al. 2017

J Clinic Toxicol

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Albizia gummifera are plants found in Ethiopia that have different medicinal values.The objective of the present study was to evaluate the toxicological effects of sub-chronic administered hydro-ethanolic (70%) seeds extract of Albizia gummiferain albino Wistar rats. The seeds of these plants were collected from different areas of Ethiopia. They were dried and crushed to powder and macerated with hydro-alcohol and placed in orbital shaker. The extract was then filtered through Whatman filter paper No.1 and the filtrate was evaporated to dryness by Rota vapor and further concentrated by water bath at 40ºC. The extract was packed in air tight brown glass bottles and kept in a refrigerator at 4ºC. The extract was then administered to rats at different doses to determine the LD 50 of the extract and at doses of 125 mg/kg/day and 250 mg/kg/day for the sub-chronic toxicity study. The LD 50 of Albizzia gummifera were found as 4000 mg/kg and 3500 mg/kg, respectively. Statistically significant difference in body weight was observed in female rats in the 10 th week at 250 mg/kg body weight of seeds extract of Albizia gummifera administered group and in the male rats at lowest dose during the 9 th and 10 th weeks of administration period for seeds extract of Millettia ferruginea.The seeds extract of Albizia gummifera statistically decreased (p ≤ 0.05) MCHin the male rats at both doses; MCHC at both 125 mg/kg and 250 mg/kg in the female rats; and MCH in the female rats at higher dose and increased RDW-CV in the male rats at both doses. It increased NEUT at the highest dose in both females and males. The seeds extract of Millettia ferruginea decreased (p ≤ 0.05) in the MCHC and MONO of female rats at the highest doses. ALP, ALT and urea were found significant in the female rats administered with 250 mg/kg of seeds extract. While, seeds extract of Albizia gummifera increased only urea in male rats at 250 mg/kg. Some histopathological changes in liver and kidney were also observed for both plants extracts. There were inflammations, congestions and focal hepatocellular necrosis of the liver tissue. The extracts also produced atrophyof the glomeruli of the kidney. The observed changes in both of the plant seeds extract might have resulted because of the presence of some bioactive ingredients in the extract. Therefore, the active ingredients which might be responsible for toxic insult should be researched with their mechanisms of actions.

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Screening of some medicinal plants of Ethiopia for their anti-microbial properties and chemical profiles

Cited by 195 publications

References 9 publications

Antifungal Activity of Some Plant Extracts against (Colletotrichum Musae) the Cause of Postharvest Banana Anthracnose

Antifungal Activity of Some Plant Extracts against (Colletotrichum Musae) the Cause of Postharvest Banana Anthracnose

Ximenia americana: Chemistry, Pharmacology and Biological Properties, a Review

Evaluations of Biochemical, Hematological and Histopathological Parameters of Subchronic Administration of Ethanol Extract of Albizia Gummifera Seed in Albino Wistar Rat

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