Screening of Rice Genotypes for Resistance to Blast Blast (Pyricularia Oryzae Cav.)

Bragina, Olesya; Ladatko, V.A.

doi:10.33775/conf-2021-163-166

Cited by 1 publication

(3 citation statements)

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“…The Basmati 217, Basmati 370, and ITA 310 were very susceptible to most isolates, this fi nding agrees with Kariaga, et al [5] and Kihoro, et al [5,7]. The accessions of Basmati 217 and Basimti 370 inbuilt by Pi9 were susceptible to the strains of blast.…”

Section: Discussionsupporting

confidence: 85%

“…Nevertheless, serious infections have already been reported in Basmati 217, Basmati 370, and ITA 310, and have become a big problem for rice cultivation in Kenya [5,7]. Basmati 217 and Basmati 370 harbored Pi20(t) and one of Pik allele genes or Pi3.…”

Section: Discussionmentioning

confidence: 99%

“…The average yield per hectare in the Mwea irrigation scheme is usually 6.5 tons of paddy rice, but due to frequent blast disease outbreaks, this may be reduced to 3 tons. Severe blast infections have been reported on Basmati 217, Basmati 370, and ITA 310 rice varieties across the rice-growing regions in Kenya [7].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of elite rice lines for resistance to Kenya blast fungus (Magnapothe Oryzae)

Roselyne U,

Rosemary,

Felister N

et al. 2024

Int J Agric Sc Food Technol

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Blast resistance tends to often break down, these necessitate search-resistant genes. The screen house experiment was conducted in 2019 and 2020. A total of 56 rice genotypes, (Elite lines, monogenic lines, and local), were screened against ten Kenya isolates of Mangnaporthe oryzae. The establishment was by direct seeding of previously sprouted seeds. A completely Randomized Design (CRD) with two replicates was adopted. Inoculation was done 21 days after planting. Plants were maintained in the moist chamber (26–28 0C) for 48 hrs, then moved into an incubation chamber (25 ◦C ± 2). Disease assessment commenced 48 hours after inoculation until 21 days to full infection. Disease evaluations were performed according to the Standard Evaluation System of IRRI. Polymerase Chain Reactions (PCR) were carried out, blast resistant loci were identified using varied genetic markers that co-segregate with specific resistant loci Analysis was implemented in R version 3.3.2 (R Core Team, 2016). BLUPs were computed, in a mixed model to estimate the random effect of the genotypes. The data was subjected to analysis of variance and means separated by Tukey’s test at P < 0.05. Cluster analyses were performed in JMP software version 11.2 for Windows. Seven genotypes (IR12A311, IR10M210, IR74, 1R02A127, IR09A130, R66 and IR10N230) were more susceptible, IR13N152 and IR14F711 were more resistant to all Isolates. The monogenic lines IRBLsh-B and IRBLkh-K3 which carry blast-resistant genes, were susceptible to at least seven Kenya isolates. The local checks (Basmati 370, BAS 270 (pi9), BW196, and BAS370 (pi9) were highly susceptible. The genotype IR12F711 was consistently resistant to all Kenya Isolates.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%