Screening of plants used in the European traditional medicine to treat memory disorders for acetylcholinesterase inhibitory activity and anti amyloidogenic activity

Lobbens, Eva S.B.; Vissing, Karina Juul; Jørgensen, Lene; Weert, Marco van de; Jäger, Anna K.

doi:10.1016/j.jep.2017.02.020

Cited by 13 publications

(5 citation statements)

References 42 publications

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“…The chloroform extract was more active than the crude extract against both enzymes (Table 1) and its AChE activity was higher than those recently reported for a species from the same family of S. argel (Vinca minor L.). 16 Therefore, this chloroform extract was chosen for further phytochemical studies. We also confirmed these results with a TLC bioautography assay and revealed several active compounds in the crude extract and the chloroform extract (Fig.…”

Section: Resultsmentioning

confidence: 99%

Chemical Constituents from Solenostemma argel and their Cholinesterase Inhibitory Activity

et al. 2019

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− Alzheimer's disease is a chronic neurodegenerative disorder with no curative treatment. The commercially available drugs, which target acetylcholinesterase, are not satisfactory. The aim of this study was to investigate the cholinesterase inhibitory activity of Solenostemma argel aerial part. Eight compounds were isolated and identified by NMR: kaempferol-3-O-glucopyranoside (1), kaempferol (2), kaempferol-3-glucopyranosyl(1→6)rhamnopyranose (3) p-hydroxybenzoic acid (4), dehydrovomifoliol (5), 14,15-dihydroxypregn-4-ene-3,20-dione (6), 14,15-dihydroxy-pregn-4-ene-3,20-dione-15β-D-glucopyranoside (7) and solargin I (8). Two of them (compounds 2 and 3) could inhibit over 50 % of butyrylcholinesterase activity at 100 µM. Compound (2) displayed the highest inhibitory effect against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) with a slight selectivity towards the latter. Molecular docking studies supported the in vitro results and revealed that (2) had made several hydrogen and π-π stacking interactions which could explain the compound potency to inhibit AChE and BChE.

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Section: Resultsmentioning

confidence: 99%

Chemical Constituents from Solenostemma argel and their Cholinesterase Inhibitory Activity

et al. 2019

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“…The experimental conditions of the in vitro AChE-inhibitory assay were based on the method described by Lobbens et al [ 101 ] with slight modifications. The acetylcholinesterase inhibitory assay was carried out in a 96-well microplate.…”

Section: Methodsmentioning

confidence: 99%

GC-MS Metabolic Profile and α-Glucosidase-, α-Amylase-, Lipase-, and Acetylcholinesterase-Inhibitory Activities of Eight Peach Varieties

et al. 2021

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The inhibition of certain digestive enzymes by target food matrices represents a new approach in the treatment of socially significant diseases. Proving the ability of fruits to inhibit such enzymes can support the inclusion of specific varieties in the daily diets of patients with diabetes, obesity, Alzheimer’s disease, etc., providing them with much more than just valuable micro- and macromolecules. The current study aimed atidentifying and comparing the GC-MS metabolic profiles of eight peach varieties (“Filina”, “Ufo 4, “Gergana”, “Laskava”, “July Lady”, “Flat Queen”, “Evmolpiya”, and “Morsiani 90”) grown in Bulgaria (local and introduced) and to evaluate the inhibitory potential of their extracts towards α-glucosidase, α-amylase, lipase, and acetylcholinesterase. In order to confirm samples’ differences or similarities, principal component analysis (PCA) and hierarchical cluster analysis (HCA) were also applied to the identified metabolites. The results provide important insights into the metabolomic profiles of the eight peach varieties and represent a first attempt to characterize the peels of the peach varieties with respect to α-glucosidase-, α-amylase-, lipase-, and acetylcholinesterase-inhibitory activities. All of the studied peach extracts displayed inhibitory activity towards α-glucosidase (IC50: 125–757 mg/mL) and acetylcholinesterase (IC50: 60–739 mg/mL), but none of them affected α-amylase activity. Five of the eight varieties showed inhibitory activity towards porcine pancreatic lipase (IC50: 24–167 mg/mL). The obtained results validate the usefulness of peaches and nectarines as valuable sources of natural agents beneficial for human health, although further detailed investigation should be performed in order to thoroughly identify the enzyme inhibitors responsible for each activity.

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“…This plant is a source of monoterpene indole alkaloids (MIAs), such as vincamine ( 3 ) (its main active compound), apovincamine, vincamone, and vincadifformine. ,− MIAs comprise a large subgroup of alkaloids with diverse biological activities, including antihypertensive (vasodilator), cytotoxicity, anti-inflammatory, and antimicrobial . The plant is traditionally used against memory loss, , and particularly the major alkaloid vincamine was marketed to enhance cerebral blood flow …”

Section: Resultsmentioning

confidence: 99%

Toward a More Sustainable Sample Preparation in Phytochemistry: Case Studies in Four Subclasses of Alkaloids

Chibli,

de Lima,

Carneiro

et al. 2024

J. Nat. Prod.

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The fact that alkaloids are bases has been the most explored chemical feature of their extraction and purification procedures. The main drawback of these procedures is that they employ undesirable chemicals, with HCl and CH 2 Cl 2 probably being the most commonly employed chemicals in their subsequent steps. This work tested the hypothesis that advantages in recovery efficiency support this common practice. Experiments were conducted in three laboratories, monitoring the alkaloids harmine (1), boldine (2), vincamine (3), and mescaline (4) extracted from Banisteriopsis caapi, Peumus boldus, Vinca minor, and Trichocereus macrogonus var. pachanoi, respectively. The research demonstrated that HCl could be replaced with citric acid (CA) without loss or even better extraction performance. The recommended EtOAc could completely replace CH 2 Cl 2 in three out of four study cases and partially in the fourth case without harming the extraction efficiency. In addition, the alternative solvents tert-amyl methyl ether (TAME) and n-butyl acetate (BuOAc) could enhance the extraction of alkaloids. These results might incentivize natural products laboratories to consider sustainability more routinely, thus being closer to current practices in the pharmaceutical industry, which has been replacing solvents and processes with greener ones.

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Screening of plants used in the European traditional medicine to treat memory disorders for acetylcholinesterase inhibitory activity and anti amyloidogenic activity

Cited by 13 publications

References 42 publications

Chemical Constituents from Solenostemma argel and their Cholinesterase Inhibitory Activity

Chemical Constituents from Solenostemma argel and their Cholinesterase Inhibitory Activity

GC-MS Metabolic Profile and α-Glucosidase-, α-Amylase-, Lipase-, and Acetylcholinesterase-Inhibitory Activities of Eight Peach Varieties

Toward a More Sustainable Sample Preparation in Phytochemistry: Case Studies in Four Subclasses of Alkaloids

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