Screening of Plant Extracts for Nemastatic Activity

Abstract

A total of 2,714 methanol extracts of 1,677 plant species in 178 families were screened for nemastatic activity against Rhabditis sp. by 96-well microplate bioassay. The plant extracts with a concentration of 5,000 μg/ml were mixed with aqueous nematode solution containing about 20 Rhabditis sp. and their activity was examined daily for 7 days. Out of 2,714 plant extracts examined in this test, 2,362 (87.0%) showed no negative influence on the nematode activity, while 187 (6.9%) inhibited nematode activity about 50%, 95 (3.5%) inhibited nematode activity over 90%, and 70 (2.6%) rather enhanced nematode activity. Among those showing over 50% nemastatic activity, 25 extracts were randomly selected for further screening with Rhabditis sp. and with juveniles of Meloidogyne incognita. The screening revealed that 11 extracts (44%) were consistently nemastatic. The 282 extracts with nemastatic activity could be of use in future studies on nematicidal plants.

Control Effect of Coffee Ground Compost and Velvet bean Against Root-Knot Nematode, Meloidogyne incognita in Pumpkin

“…뿌리혹선충은 식물의 뿌리 세포들로부터 직접 양분을 흡수하는데 토양 내 선충 밀도의 증가와 식물에 나 타나는 피해 정도는 높은 상관관계를 가지는 것으로 알려져 있다 . 현재 시설재배지에서 뿌리혹선충을 방제하기 위하여 저항 성 품종 (Baek, 2004;Kim et al, 2010), 작부체계 변경 (Bridge, 1996), 토양 개량 (Park et al, 1995), 태양열소독 (Chon et al, 1996), 화학적 방제 (Kim et al, 2015), 생물학 적 방제 (Ha et al, 2014;Liang et al, 2010;Park et al, 2004;Radwan et al, 2013), 식물추출물 (Bawa et al, 2014;Elbadri, et al, 2014;Kim et al, 1998;Kim et al, 2014) 등의 방제기술들이 개발되고 있다 . 화 학합성 농약은 적은 양으로도 효과가 좋고 가격이 저렴하여 소득증대에 큰 역할을 했지만, 계속 문제가 되어 왔던 환경 오염, 농작물의 잔류 독성 등의 문제가 사회적으로 부각되 었다 (Chitwood, 2002).…”