Screening of Phytochemicals and in Vitro Antidiabetic Activity of Bauhinia Racemosa Lam. Leaves

Gawade, Bhimraj; Farooqui, Mazahar

doi:10.22159/ajpcr.2018.v11i6.24038

Cited by 13 publications

(10 citation statements)

References 9 publications

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“…Antibacterial activity of the ethanol, methanol, and aqueous extracts was screened by agar well diffusion method as described by Gawade and Farooqui [20] and Abdul et al [21]. Briefly, the bacterial suspension was swabbed uniformly to the Petri dishes containing 20-30 Mueller-Hinton Agar (MHA), and the inoculum was allowed to dry for 5-10 min.…”

Section: In Vitro Antibacterial Bioassaymentioning

confidence: 99%

Anticancer, Cytotoxic Effect of Tamarix Aphylla, and Antibacterial Screening Efficiency Against Multidrug-Resistant Human Pathogens

Sobeai

2018

Asian J Pharm Clin Res

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Objectives: The present study aimed to investigate the anticancer, cytotoxic effect of Tamarix aphylla, and antibacterial effectiveness against 10 pathogenic bacteria that cause common and sometimes serious infections in human and animals.Methods: T. aphylla fresh and disease-free leaves were collected from the different geographical regions of Saudi Arabia. The anticancer, cytotoxic effect of T. aphylla leaves, and antibacterial screening efficiency against multidrug-resistant human pathogens were investigated in vitro using Vero cells as a normal cells and MCF-7 (breast adenocarcinoma cells).Results: T. aphylla leaf extracts exhibited a low cytotoxic effect on Vero cell line at high concentration, with an 50% cytotoxicity concentration value of >1000 μg/ml. The methanolic extract inhibited MCF-7 cancer cells in a concentration-dependent manner. The methanol and ethanol extracts showed antibacterial activity with variable inhibition effects and differences in their activities against tested pathogenic bacteria ranging from very high inhibition (20.7±1.3 mm) to low (4±0.6 mm). The minimum inhibitory concentration of methanol and ethanol results showed no significant differences.Conclusions: The findings of this study conclude that the T. aphylla leaf extract had lower toxicity on normal cell line (low toxic plant) and significantly inhibited the growth of cancer cells. T. aphylla has potential antibacterial biomolecules against multidrug-resistant human pathogens.

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Section: In Vitro Antibacterial Bioassaymentioning

confidence: 99%

Anticancer, Cytotoxic Effect of Tamarix Aphylla, and Antibacterial Screening Efficiency Against Multidrug-Resistant Human Pathogens

Sobeai

2018

Asian J Pharm Clin Res

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“…The major portion of the extract inositol. In a study, GC-MS Bauhinia racemosa leaves revealed inositol and the extract exhibited In-vitro anti-diabetic activity [23].…”

Section: Effect Of Various Treatments On Blood Glucose Level Of Experimental Ratsmentioning

confidence: 98%

GC-MS Analysis of Chemical Constituents of Hydroalcoholic Leaf Extract of Cissampelos Pareira and Their Anti-Diabetic Activity

Asif¹,

Gilani

Taleuzzaman³

et al. 2021

APRJ

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Aim: The present work deals with the GC-MS-analysis of chemical constituents of hydroalcoholic extract of Cissampelos pareira leaves and thier anti-diabetic activity. Methods: GC-MS analysis of extract was performed using Shimadzu QP-2010 plus with thermal desorption system 20. Acute oral toxicity of extract was done using the Organization of Economic Co-operation and Development (OECD) guideline 423. Diabetes was induced by single dose of streptozotocin 65 mg/kg, i.p. to all the rats except in rats of control group. Following which oral glucose tolerance test was performed and the rats were divided into various experimental groups. Various treatments continued for 21 days. Parameters such as blood glucose level, body weight, liver enzymes, lipid profiles and oxidative markers were checked. Results: GC-MS analysis of the extract identified 25 compounds present in it. Based on acute oral toxicity study three doses of hydroalcoholic extract of Cissampelos pareira leaves viz 100, 200 and 400 mg/kg were selected for evaluation of anti-diabetic activity. The extracts at doses 200 and 400 mg/kg BW were able to reduce blood sugar level, liver enzymes, total cholesterol, total triglyceride, low density lipoprotein and Malondialdehyde; and enhance body weight, high density lipoprotein and Glutathione significantly when compared to rats of negative control group. The effect of extract at dose 400 mg/kg was comparable to standard Glibenclamide. Conclusion: Results conclude that the chemical constituents present in the hydroalcoholic extract of Cissampelos pareira contained some anti-diabetic compounds possessing strong anti-diabetic activity.

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“…The extract was preliminary qualitatively analysed for the bioactive phyto-constituents according to the standard protocols [11,12] as…”

Section: Phytochemical Analysismentioning

confidence: 99%

Antioxidant Potential and GC-MS Analysis of Abrus precatorius Linn Leaves Ethanol Extract

Gawade¹,

Farooqui²

2021

CSIJ

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Aims: The aim of present study to evaluate the antioxidant potential of Abrus precatorius Linn leaves ethanol extract containing bioactive compounds on free radical scavenger using DPPH. Ethanol extract of Abrus precatorius Linn leaves was subjected to preliminary phytochemical screening and fraction of extract was detected by Gas Chromatography-Mass Spectrometry analysis. Methodology and Results: GC-MS analysis of ethanol leaves extract was carried out on Shimadzu GC-MS model number QP 2010S and revealed the presence of 18 phytochemical compounds, with mome inositol as a dominant component. Antioxidant potential of leaves ethanol extract was evaluated using DPPH free radical scavenging assay at five different doses as 5, 10, 20, 30, 40 and 50 μg/mL and showed significant DPPH free radical scavenging potential with the IC50 value of 33.37 μg/mL. The bioautography of extract showed that fractions with the most prominent antioxidant potential tended to contain secondary metabolites reported in preliminary phytochemical screening such as alkaloids, carbohydrates, protein and amino acids, glycoside, tannins, flavonoids, triterpenoids and phenolic compounds. The results evaluate and justify the traditional relevance of Abrus precatorius Linn leaves ethanol extract for free radical scavenging potential as a antioxidant and can be used as a lead for the isolation of the antioxidant bioactive chemical constituents in further study. Conclusion: Preliminary phytochemical screening reported various bioactive compounds in the ethanol extract of Abrus precatorius Linn leaves and identified by GC-MS. The extract exhibited greater free radical scavenging activity i.e. antioxidant potential. The presence of various bioactive phytocompounds justifies the therapeutic use of the Abrus precatorius Linn plant leaves for various ailments by traditional practitioners.

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Screening of Phytochemicals and in Vitro Antidiabetic Activity of Bauhinia Racemosa Lam. Leaves

Cited by 13 publications

References 9 publications

Anticancer, Cytotoxic Effect of Tamarix Aphylla, and Antibacterial Screening Efficiency Against Multidrug-Resistant Human Pathogens

Anticancer, Cytotoxic Effect of Tamarix Aphylla, and Antibacterial Screening Efficiency Against Multidrug-Resistant Human Pathogens

GC-MS Analysis of Chemical Constituents of Hydroalcoholic Leaf Extract of Cissampelos Pareira and Their Anti-Diabetic Activity

Antioxidant Potential and GC-MS Analysis of Abrus precatorius Linn Leaves Ethanol Extract

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