Screening of phage‐displayed human liver cDNA library against doxorubicin with drug‐immobilized monolithic polyacrylamide cryogel

Abstract

Monolithic polyacrylamide cryogel was prepared and utilized as a new matrix for drug immobilization to screen against phage-displayed human liver cDNA library. The macropores and hydrophilic nature of the cryogel made it possible for phage particles to pass unhindered. Doxorubicin, an anticancer drug, was covalently bonded to the monolithic cryogel by the glutaraldehyde method, and after five rounds of affinity selection performed in an SPE cartridge, phage clones that displayed Homo sapiens methyl CpG binding protein 2 (MeCP2 ) were selectively enriched. The interaction between doxorubicin and MeCP2 displayed phages was further validated by studying the retention of doxorubicin on MeCP2 phage-coupled cryogel. These results demonstrate that drug-coupled polyacrylamide cryogel might be a promising kind of matrix for screening target proteins against phage-displayed library.

Biomolecule-Conjugated Macroporous Hydrogels for Biomedical Applications

“…Drug release studies using CH revealed a pH-dependent release due to the hydrolysis of carbamate groups under acidic conditions, leading to increased drug release being observed (Figure bi). It was reported that doxorubicin can be covalently attached to the polyacrylamide cryogel by using glutaraldehyde method . The pH-responsive delivery of doxorubicin can also be achieved using a molecular imprinted cryogel made up of HEMA and gelatin, which showed increased drug release at tumor sites .…”

“…It was reported that doxorubicin can be covalently attached to the polyacrylamide cryogel by using glutaraldehyde method. 213 The pH-responsive delivery of doxorubicin can also be achieved using a molecular imprinted cryogel made up of HEMA and gelatin, which showed increased drug release at tumor sites. 214 A doxorubicinfunctionalized multifunctional scaffold made of PLGAchitosan-silica was also fabricated to check the potency and to estimate the antiproliferative effect of the drug on HeLa cells.…”

One-pot preparation of a sulfamethoxazole functionalized affinity monolithic column for selective isolation and purification of trypsin

“…Most drugs produce their biological effects by interacting with specific macromolecular components of the organism. As a consequence, drug ligand based affinity columns could be an interesting and useful model for studying drug-receptor interaction and purifying targeted proteins [22][23][24]. Compared to macromolecule functionalized affinity columns, small drug molecule functionalized affinity columns also have many advantages such as good stability, long life, low cost, and easy preparation.…”

“…As an alternative option, small molecule ligands, such as chelated metal ions [16], amino acids [17], bioactive compounds [18], triazine dyes [19][20][21] and drugs [22][23][24] have proved to be efficient ligands for isolating the target proteins by affinity chromatography. Most drugs produce their biological effects by interacting with specific macromolecular components of the organism.…”

“…Mattiasson and co-workers [22] developed sulfamethazine modified monolithic gels, which exhibited specific binding to IgG-and IgYtreated inclusion bodies. Yu and Zhang [23] developed doxorubicin functionalized polyacrylamide based monoliths in SPE cartridges using either the glutaraldehyde or the epoxy method. Both kinds of columns were used for screening T7-phage display human liver cDNA library.…”