One-pot preparation of a sulfamethoxazole functionalized affinity monolithic column for selective isolation and purification of trypsin

Xiao, Ying; Guo, Jialiang; Ran, Danni; Duan, Qianqian; Crommen, Jacques; Jiang, Zhengjin

doi:10.1016/j.chroma.2015.04.046

Cited by 8 publications

(7 citation statements)

References 31 publications

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“…Additionally, H‐bonds were also formed between scutellarin and Ser 214 , baicalin and Asp 189 , wogonoside and Cys 191 (Supporting Information Figure S3C). It was reported that the affinity model of BH towards trypsin was based on H‐bonds formed with Asp 189 , Ser 190 , Cys 191 , Ser 195 , Ser 214 , and Gly 219 . The results indicate that the interactions of the three compounds with trypsin are similar to those of BH.…”

Section: Resultsmentioning

confidence: 82%

“…However, the preparation and efficient packing of affinity SPE columns is complicated and time consuming. Polymeric monoliths have been regarded as a useful alternative material to immobilize biomolecules for bio‐interaction studies , proteomic studies , purification as well as bioactivity screening , because of their high extraction efficiency, satisfactory physicochemical properties and biocompatibility . The capillary IMER can minimize the consumption of biomolecules, especially for those rare biomolecules.…”

Section: Introductionmentioning

confidence: 99%

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A strategy for screening trypsin inhibitors from traditional Chinese medicine based on a monolithic capillary immobilized enzyme reactor coupled with offline liquid chromatography and mass spectrometry

Lin

Zhang

Yang

et al. 2019

J of Separation Science

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† These authors contributed equally to this work.A novel strategy was successfully developed for screening trypsin inhibitors in traditional Chinese medicines based on monolithic capillary immobilized enzyme reactors combined with liquid chromatography-tandem mass spectrometry. Organic polymer based monolithic enzyme reactors were firstly prepared by covalently bonding trypsin to a poly(glycidyl methacrylate-co-poly (ethylene glycol) diacrylate) monolith by the ring-opening reaction of epoxy groups. The activity and kinetic parameters of the obtained monolithic trypsin reactors were systematically evaluated using microliquid chromatography. Fourier transform infrared spectroscopy and scanning electron microscopy were also used to characterize the monolithic trypsin reactors. The resulting functional and denatured monolithic trypsin reactors were applied as affinity solidphase extraction columns, and offline coupled with a liquid chromatography-tandem mass spectrometry system to construct a binding affinity screening platform. Subsequently, the proposed platform was applied for screening trypsin binders in a Scutellaria baicalensis Georgi extract. Three compounds, namely scutellarin, baicalin, and wogonoside were identified, and their inhibitory activities were further confirmed via an in vitro enzymatic inhibition assay. Additionally, molecular docking was also performed to study the interactions between trypsin and these three compounds.

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Section: Resultsmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

A strategy for screening trypsin inhibitors from traditional Chinese medicine based on a monolithic capillary immobilized enzyme reactor coupled with offline liquid chromatography and mass spectrometry

Lin

Zhang

Yang

et al. 2019

J of Separation Science

Self Cite

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“…Before the preparation of monolithic columns, the inner wall of the fused‐silica capillaries was modified with a layer of γ‐MAPS using previously reported methods . The schematic preparation of β‐CD‐functionalized monolithic columns for the three approaches is illustrated in Fig.…”

Section: Methodsmentioning

confidence: 99%

Effect of fabrication strategy on the enantioseparation performance of β‐cyclodextrin‐functionalized polymethacrylate monoliths: A comparative evaluation

Yang

Guo

Lin

et al. 2017

J of Separation Science

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To evaluate the effect of the preparation strategy on the enantioseparation performance of β-cyclodextrin-functionalized monoliths, a series of β-cyclodextrin-functionalized organic polymeric monolithic columns were prepared through two-step, single-step, and one-pot approaches, using the same cyclodextrin, linker-spacer, and crosslinker. Physicochemical characterization of the columns was carried out by determining the morphology, β-cyclodextrin density, permeability, and chromatographic efficiency. For each type of monolithic column, the enantioresolution of 22 chiral compounds, including mandelic acid derivatives, nonsteroidal anti-inflammatory drugs, N-derivatized amino acids, and herbicides, was comparatively studied under optimum chromatographic conditions. The β-cyclodextrin-functionalized monolithic columns prepared through the one-pot approach exhibited higher enantioresolution for most chiral compounds, and they have the advantage of good controllability and simple preparation. On the other hand, the enantioresolution obtained on columns prepared through the single-step approach was quite unsatisfactory, and therefore the effect of using different linking spacers and crosslinkers was studied. A significant improvement of enantioresolution for 2-chloro-mandelic acid was obtained by using N,N-methylenebisacrylamide instead of ethylene dimethacrylate as the crosslinker in the single-step preparation.

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“…The IMAC format in AMC has been utilized in several studies for the purification and analysis of proteins, enzymes, N‐glycans, peptides, and DNA [134,159,210,275–284]. For instance, a hybrid cryogel containing Cu 2+ on bentonite particles has been used to bind hemoglobin from aqueous solutions (see Fig.…”

Section: Applications Of Amcmentioning

confidence: 99%

Affinity monolith chromatography: A review of general principles and recent developments

2021

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Affinity monolith chromatography (AMC) is a liquid chromatographic technique that utilizes a monolithic support with a biological ligand or related binding agent to isolate, enrich, or detect a target analyte in a complex matrix. The target‐specific interaction exhibited by the binding agents makes AMC attractive for the separation or detection of a wide range of compounds. This article will review the basic principles of AMC and recent developments in this field. The supports used in AMC will be discussed, including organic, inorganic, hybrid, carbohydrate, and cryogel monoliths. Schemes for attaching binding agents to these monoliths will be examined as well, such as covalent immobilization, biospecific adsorption, entrapment, molecular imprinting, and coordination methods. An overview will then be given of binding agents that have recently been used in AMC, along with their applications. These applications will include bioaffinity chromatography, immunoaffinity chromatography, immobilized metal‐ion affinity chromatography, and dye‐ligand or biomimetic affinity chromatography. The use of AMC in chiral separations and biointeraction studies will also be discussed.

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One-pot preparation of a sulfamethoxazole functionalized affinity monolithic column for selective isolation and purification of trypsin

Cited by 8 publications

References 31 publications

A strategy for screening trypsin inhibitors from traditional Chinese medicine based on a monolithic capillary immobilized enzyme reactor coupled with offline liquid chromatography and mass spectrometry

A strategy for screening trypsin inhibitors from traditional Chinese medicine based on a monolithic capillary immobilized enzyme reactor coupled with offline liquid chromatography and mass spectrometry

Effect of fabrication strategy on the enantioseparation performance of β‐cyclodextrin‐functionalized polymethacrylate monoliths: A comparative evaluation

Affinity monolith chromatography: A review of general principles and recent developments

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