Screening of penicillin residues in milk by a surface plasmon resonance-based biosensor assay: comparison of chemical and enzymatic sample pre-treatment

Gaudin, Valérie; Fontaine, Jéhanne; Maris, P.

doi:10.1016/s0003-2670(01)00948-5

Cited by 58 publications

(21 citation statements)

References 14 publications

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“…They produce rapid and reliable results with minimal sample preparation and use of analyte (Elliott et al, 1999;Gaudin et al, 2001;Gustavsson et al, 2002;Haasnoot et al, 2002;Mello and Kubota, 2002;Samsonova et al, 2002;Ferguson et al, 2002Ferguson et al, , 2005Gaudin et al, 2005;Traynor et al, 2006;Mauriz et al, 2006;Llamas et al, 2007;Campbell et al, 2007;Connolly et al, 2007, Campas et al, 2007. This technology allows for real time, automated analysis combining the high affinity of biochemical interactions with low limits of detection and is characterised by its simplicity of use.…”

Section: Introductionmentioning

confidence: 99%

Development of a monoclonal antibody binding okadaic acid and dinophysistoxins-1, -2 in proportion to their toxicity equivalence factors

Stewart

Elliott

Walker³

et al. 2009

Toxicon

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a b s t r a c tOkadaic acid (OA) and structurally related toxins dinophysistoxin-1 (DTX-1), and DTX-2, are lipophilic marine biotoxins. The current reference method for the analysis of these toxins is the mouse bioassay (MBA). This method is under increasing criticism both from an ethical point of view and because of its limited sensitivity and specificity. Alternative replacement methods must be rapid, robust, cost effective, specific and sensitive. Although published immuno-based detection techniques have good sensitivities, they are restricted in their use because of their inability to: (i) detect all of the OA toxins that contribute to contamination; and (ii) factor in the relative toxicities of each contaminant. Monoclonal antibodies (MAbs) were produced to OA and an automated biosensor screening assay developed and compared with ELISA techniques. The screening assay was designed to increase the probability of identifying a MAb capable of detecting all OA toxins. The result was the generation of a unique MAb which not only cross-reacted with both DTX-1 and DTX-2 but had a cross-reactivity profile in buffer that reflected exactly the intrinsic toxic potency of the OA group of toxins. Preliminary matrix studies reflected these results. This antibody is an excellent candidate for the development of a range of functional immunochemical-based detection assays for this group of toxins.

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Section: Introductionmentioning

confidence: 99%

Development of a monoclonal antibody binding okadaic acid and dinophysistoxins-1, -2 in proportion to their toxicity equivalence factors

Stewart

Elliott

Walker³

et al. 2009

Toxicon

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“…First methods were based on detection of growth inhibition of various sensitive bacterial strains. Besides these microbial inhibition tests, microbial receptor assay [11], enzyme immunoassay method [12], receptor binding assay [13], chromatographic analysis [14], surface plasmon resonance [15], and mass spectrometry [16] have been employed for this purpose.…”

Section: Introductionmentioning

confidence: 99%

Square-wave voltammetric determination of cefoperazone in a bacterial culture, pharmaceutical drug, milk, and urine

Billová

Kizek

Jelen

et al. 2003

Analytical and Bioanalytical Chemistry

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Use of square-wave voltammetry (SWV) for determination of cefoperazone (CFPZ) in some buffers, bacterial culture, urine, and milk is described. CFPZ provides a specific voltammetric signal which is affected by pH and solution components. Determination of CFPZ in Britton-Robinson buffer, pH 4.4, is sensitive with a low detection limit (about 0.5 nmol L(-1)). In a more complex medium (bacterial 2YT medium, pH 7.2) the detection limit was approximately 1.5 micromol L(-1). We provide evidence that SWV is a suitable and quick method for CFPZ determination in a culture of living bacteria without separation of biomass. We have found big differences between methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive Staphylococcus aureus (MSSA) in cultivation in the presence of CFPZ, depending on time. When CFPZ is cleaved by penicillinase, a new SWV peak b appears at more positive potentials. This peak rises both with increasing concentration of enzyme and with cleavage time while the original CFPZ peak is simultaneously decreasing. We determined the concentration of CFPZ in the drug Pathozone by the standard addition method and achieved good agreement with the declared value of CFPZ in the drug. With a simple pretreatment procedure it is possible to determine CFPZ in milk; for urine no pretreatment was required. Using SWV we could detect CFPZ concentrations as low as 500 nmol L(-1) in bovine milk and human urine.

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“…However, this was used to screen for open-ringed structures, requiring enzymatic or chemical pretreatment of the samples. In that work, the biosensor did not reach the MRL [45]. A portable wavelength-interrogated optical system (WIOS) exploited class-selective bioreceptors for simultaneous screening of the most frequently used veterinary antibiotics (e.g., sulfonamides, fluoroquinolones, β-lactams and tetracyclines) [46].…”

Section: Optical Biosensorsmentioning

confidence: 99%

“…The advantage is that only intact β-lactam structures are detected. The Protein conjugates of the haptens were immobilized as spots on disposable chips, which were processed in a flow cell 5 min [74] Ab/Ag Optical Used electrodes modified with silver nanoparticles (AgNPs) that allowed for an increase in the amount of immobilized antibodies [75] Ab/Ag Optical a 7.8 ng mL −1 Amount of nanogold-labelled immunocomplex formed increased on addition of penicillin G [47] Ab/Ag Optical a 4 Enzymatic and chemical pretreatment [45] Ab/Ag Optical 10 ng mL −1 Two penicillin-specific monoclonal antibodies and a penicillinspecific polyclonal antibody [44] Ab/Ag Electrochemical c 5…”

Section: Optical Biosensorsmentioning

confidence: 99%

Current bioanalytical methods for detection of penicillins

et al. 2012

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Screening of penicillin residues in milk by a surface plasmon resonance-based biosensor assay: comparison of chemical and enzymatic sample pre-treatment

Cited by 58 publications

References 14 publications

Development of a monoclonal antibody binding okadaic acid and dinophysistoxins-1, -2 in proportion to their toxicity equivalence factors

Development of a monoclonal antibody binding okadaic acid and dinophysistoxins-1, -2 in proportion to their toxicity equivalence factors

Square-wave voltammetric determination of cefoperazone in a bacterial culture, pharmaceutical drug, milk, and urine

Current bioanalytical methods for detection of penicillins

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