Screening of high lipase producing Candida sp. and production of lipase by fermentation

Production of an extracellular lipase by Candida utilis NRRL-Y-900 using agro-industrial by-products

“…Among inorganic nitrogen sources used, ammonium sulphate (0.4%, w/v) was the optimal inorganic nitrogen source, which increased lipase activity (Fig 6B) because active site of enzyme might be influenced by ammonium sulphate or the ammonium ions [35]. The same result was reported by Tan et al [36].…”

Pilot-Scale Production of Lipase Using Palm Oil Mill Effluent as a Basal Medium and Its Immobilization by Selected Materials

“…With regard to aeration conditions, lipase produced using an aeration rate of 0.5 vvm had an activity higher than that of lipase produced using an aeration rate of 1 vvm. These results suggest that the DO saturation level is important since excess air did not stimulate lipase production 23 .…”

“…Tan et al 23 showed that a high agitation speed 400 rpm increased the DO in the medium and enhancedthe lipase production of the mutant strain Candida sp. 99-125; the maximum activity of the produced lipase was higher than that seen with a low agitation speed of 200 rpm.…”

Immobilization of Lipase on Epoxy Activated (1→3)-α-D-Glucan Isolated fromPenicillium chrysongenum

“…WIG and Candida sp. lipase (EC3.1.1.3) (specific activity 28 U/mg) were made in our laboratory as described by Tan et al 17,18) Sepharose was purchased from Pharmcia (Uppsala, Sweden). BSA (!…”