Pilot-Scale Production of Lipase Using Palm Oil Mill Effluent as a Basal Medium and Its Immobilization by Selected Materials

Asih, Devi Ratna; Alam, Md. Zahangir; Salleh, Noor; Salihu, Aliyu

doi:10.5650/jos.ess13187

Cited by 5 publications

(3 citation statements)

References 28 publications

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“…Thus screening of microorganisms with lipolytic activities in extreme habitats could aid the discovery of novel lipases. This is the first report of this lipase-producing mould from a palm oil contaminated soil, although there have been reports on lipases produced by fungi from palm oil mill effluent (POME) [22][23][24][25]. This study will provide information on a microbial organism which can secrete lipase for industrial purposes.…”

Section: Introductionmentioning

confidence: 99%

Studies on the Optimization of Lipase Production by Rhizopus sp. ZAC3 Isolated from the Contaminated Soil of a Palm Oil Processing Shed

Ayinla¹,

Ademakinwa²,

Agboola³

2017

J App Biol Biotech

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This study investigated the screening, production and optimization of an extracellular lipase from a fungus isolated from the contaminated soil of a palm oil processing shed. This was with a view to obtaining a strain that can secrete lipase with biochemical properties exploitable for biotechnological applications such as bioremediation of oil contaminated sites. Soil samples were collected from palm oil contaminated sites in Gbogan, Osun State, Nigeria (Latitude N 7°29.1481´ and Longitude E 4°20.7587´). The isolated fungal strains were screened on tributyrin agar for exogenous lipolytic activity. Molecular identification was carried out by amplifications of ITS-1, 5.8S and ITS-2 regions. The effects of incubation time, inducers, pH, temperature, carbon and nitrogen sources were varied for optimal lipase production using one factor at a time approach. Rhizopus oryzae ZAC3 (NCBI accession No: KX035094) was identified as the highest lipaseproducing strain. Maximum lipase production was observed on the fourth day, pH 5.0 and a temperature of 45 o C. Olive oil, xylose and yeast extract were the best inducer, carbon and nitrogen sources respectively for lipase production. There was a 2.02 fold increase in lipase production under these optimized conditions. In conclusion, Rhizopus oryzae ZAC3 lipase has properties exploitable for industrial and biotechnological applications.

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Section: Introductionmentioning

confidence: 99%

Studies on the Optimization of Lipase Production by Rhizopus sp. ZAC3 Isolated from the Contaminated Soil of a Palm Oil Processing Shed

Ayinla¹,

Ademakinwa²,

Agboola³

2017

J App Biol Biotech

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“…Furthermore, Salihu and coworkers (20) reported the production of lipase by Candida cylindracea using effluent oil obtained in refining industry. Asih and colleagues (21) reported the production of lipase by Candida cylindracea using palm oil mill effluente.…”

Section: Font: Authorsmentioning

confidence: 99%

Utilization of agroindustrial byproducts for the production of lipase by a new strain of Pseudomonas sp.

Baldo

Baggio

Oliveira

et al. 2020

Semin. Cienc. Biol. Saude

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Este estudo teve como objetivo avaliar a produção de lipases por uma nova cepa de Pseudomonas sp. utilizando meio de fermentação contendo subprodutos de industrialização de carne de frango e óleo de soja. Os resultados indicaram que a gordura de frango e a goma de soja induziram 48,3 U/mL e 93,3 U/ml de atividade lipásica, respectivamente. No entanto, a produção de lipase mais elevada foi obtida quando a goma de lecitina bruta foi utilizada, induzindo 272,6 U/ml de atividade após 24 horas. A caracterização bioquímica parcial da enzima mostrou que as condições de reação ótimas foram de pH 9,0 e 35°C. A enzima foi estável nas temperaturas entre 25 a 75°C e pH de 6 a 9. A enzima mostrou boa estabilidade em solventes orgânicos tais como acetonitrila, hexano, etanol e isopropanol. Este estudo indicou que os subprodutos testados são promissores para a produção de lipase e podem contribuir para a redução dos custos de produção enzimática em larga escala, aumentar o valor desses subprodutos e reduzir potenciais impactos ambientais causados por sua acumulação na natureza.

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“…[1][2][3] They play an important role in the breakdown of fats and oils with a subsequent release of long-chain triglycerides, diglycerides, monoglycerides, or free fatty acids. [4][5][6] They react in a wide range of reversible conversion reactions, which is why they have been studied extensively for potential industrial applications such as biocatalysts in biodiesel synthesis, in oleochemicals, in pharmaceuticals, in biomedical assays, and for use in food additives. [7][8][9] Lipases are derived from nature and can be produced by microbial fermentation for different applications.…”

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confidence: 99%

Utilizing palm oil mill effluent (POME) for the immobilization of Aspergillus oryzae whole‐cell lipase strains for biodiesel synthesis

Rachmadona

Quayson

Amoah

et al. 2021

Biofuels Bioprod Bioref

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Palm oil mill effluent (POME) is a palm industrial waste by‐product that is readily available and possesses high quantities of the organic compounds that are necessary for microbial growth. This study investigated the potential of immobilized Aspergillus oryzae whole cells expressing Candida antartica lipase B (r‐CALB) when using a POME‐based medium as a low‐cost carbon source within biomass support particles. The dry cell weight, hydrolytic activity, and remaining protein were measured for a variety of POME (0–2% w/v) and carbon source concentrations (0–2% w/v) with an incubation time of 96 h. Using POME in the culture medium as a carbon source instead of glucose doubled the retention of cell weight from 1.16 ± 0.03 to 2.36 ± 0.02 g within the biomass support particles and improved the hydrolytic activity from 1.61 ± 0.02 to 2.23 ± 0.02 U mg−1 in whole‐cell lipase. This immobilized r‐CALB was used as a catalyst to esterify POME into biodiesel. This synthesis produced an ester content of 97.52 ± 0.21% w/w after 96 h. Immobilized r‐CALB was reusable for more than ten batches, and resulted in an ethyl ester content of more than 90% w/w even after 10 cycles. The present study demonstrated that POME could serve as a sustainable carbon source for industrial microbial culture with the additional incentive of being a treatment that is inexpensive and sustainable. © 2021 Society of Chemical Industry and John Wiley & Sons, Ltd

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Pilot-Scale Production of Lipase Using Palm Oil Mill Effluent as a Basal Medium and Its Immobilization by Selected Materials

Cited by 5 publications

References 28 publications

Studies on the Optimization of Lipase Production by Rhizopus sp. ZAC3 Isolated from the Contaminated Soil of a Palm Oil Processing Shed

Studies on the Optimization of Lipase Production by Rhizopus sp. ZAC3 Isolated from the Contaminated Soil of a Palm Oil Processing Shed

Utilization of agroindustrial byproducts for the production of lipase by a new strain of Pseudomonas sp.

Utilizing palm oil mill effluent (POME) for the immobilization of Aspergillus oryzae whole‐cell lipase strains for biodiesel synthesis

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