Screening of fungal isolates and properties of Ganoderma applanatum intended for olive mill wastewater decolourization and dephenolization

Abstract

Aims: To investigate different autochthonous isolates of wood‐rotting fungi for the removal of both colour and phenolic compounds from olive mill wastewaters (OMW). Methods and Results: The isolates Bjerkandera adusta Ba‐100, Fomes fomentarius Ff‐106, Ganoderma applanatum Ga‐20, Irpex lacteus Il‐3, Trametes versicolor Tv‐101 and Tv‐103 were preliminarily screened for their OMW‐decolourizing potential on potato dextrose agar supplemented with different OMW concentrations. A further screening of batch cultures under different agitation speeds, to test the effect of shear stress, resulted in the selection of isolate G. applanatum Ga‐20. Batch cultures grown in OMW‐based medium exhibited strong laccase induction and significant decrease in the values of phenols, colour and chemical oxygen demand. Concomitant onset of laccase activity and colour removal was observed, and apart from laccase, neither lignin peroxidase nor manganese‐dependent peroxidase activities were detected. Moreover, the depletion of aromatic compounds with high and low apparent molecular mass was observed by chromatographic analysis. Conclusions: Isolate G. applanatum Ga‐20 exhibited interesting properties for its use in bioremediation of OMW, namely high removal of recalcitrant phenolic compounds and strong colour abatement. Significance and Impact of the Study: For the first time, the white‐rot fungus G. applanatum proves to be effective for the decolourization and dephenolization of OMW.

Characterization of the Oxidative Enzyme Potential in Wild White Rot Fungi From Misiones (Argentina)

“…These results were in agreement with others previously reported by Tekere et al (2001a), Saparrat et al (2002), Matos et al (2007), Lin et al (2003) and Basto et al (2006) who also described high ligninolytic activities in closely related fungal specimens such as T. villosa, C. versicolor, G. applanatum and P. sanguineus, consequently proposed for different biotechnological applications.…”

Comparative Examination of the Olive Mill Wastewater Biodegradation Process by Various Wood-Rot Macrofungi

“…The latter was the second fastest (after A. biennis ) in reducing total phenols since a 49% decrease was measured after just 12 days of incubation. It is noteworthy that other Ganoderma species/strains were reported to present similar behavior as regards the time needed to decrease to a similar extent OMW's phenolics, that is, by 41–44% within a 10-day incubation period and by reaching a total reduction exceeding 64% [26, 32]. On the other hand, selected P. ostreatus and P. eryngii strains demonstrated a rather delayed phenols degradation which exceeded the 50% level only after the completion of the first half of growth [32] much like as it was observed for P. djamor and P. pulmonarius in the present study.…”

“…In other pertinent studies, Pleurotus spp., Ganoderma applanatum , Lentinula edodes, Pycnoporus coccineus, Coriolopsis polyzona, and Lentinus tigrinus demonstrated similar magnitude of decolorization; however, in several of these cases initial medium and cultivation conditions varied considerably [26, 32, 49, 50]. In addition, the outcome of this study demonstrates that decolorization is significantly correlated with total phenol reduction ( r 2 = 0.71), which is in line with previous results associating OMW decolorization with the degradation of high molecular-mass polyphenols [47, 51].…”

Irpex lacteus, a white-rot fungus with biotechnological potential — review

“…lacteus has been screened together with other fungal strains both for degradation of individual organic pollutants including PAHs, synthetic dyes and endocrine disruptors, and for decolorization of industrial wastewaters (Song 1997;Šašek et al 1998;Novotný et al 2001;Aggelis et al 2002;Ricci et al 2005;Rigas and Dritsa 2006;Matos et al 2007;Hwang et al 2008) (Table II).…”