Screening of Functional Materials from Solvent Fractions of Apple Flower Leaf Extract

Abstract

1) Fractional solvent extraction by organic solvents such as hexane, chloroform, ethylacetate, and butanol was carried out using 70% ethanol extract of apple flower leaves. Biological activities including antioxidant, whitening, antimicrobial and anti-wrinkle activities were investigated and bio-active materials of the extracts were identified using GC/MSD. Among the tested solvent fractions, ethylacetate fraction showed the highest total polyphenol content (1218.94 μg/mL), and flavonoid (140 μg/mL). The DPPH radical scavenging activities was over 80% at a dry matterbased concentration of 200 μg/μL and SOD-like activity was over 90% at 50 μg/mL concentration in ethylacetate fraction that was slightly lower than of ascorbic aicd. Tyrosinase inhibition activity related to skin-whitening was over 60% by ethylacetate fraction of 100 μg/mL. As an anti-aging effect, elastase inhibitory activity was about 45% in ethylacetate fraction. Also, it showed a significantly antimicrobial activity against P. acenes. From GC/MSD analysis, a characteristic peak of high content in ethylacetate fraction was identified as kaempferol, which has been reported as a bioactive compound.

Anti-inflammatory Activity of Solvent Fractions from Ginseng Berry Extract in LPS-Induced RAW264.7 Cells

“…NO는 외부상처에 대한 반응 및 염증 같은 면역방어기전의 Choi et al, 2011;Park and Yang, 2008;Lee et al, 2012;Byun, 2012). 일반적으로 ethylacetate분획물에서 검출되어지는 폴리 페놀성 물질이나 플라보노이드 물질이 대부분 많아서 이러한 물질이 항산화 및 항염증 활성에 관여한다고 하지만 (Cha et al, 1999;Lu and Foo, 2000;Tsao, 2010 어지게 된다 (Hawiger 2001;Nam, 2006 …”

Functional Characterization of Callus Extracts of Apple 'Hirosaki' for Cosmetic Materials

“…[3]. 꽃잎 추출물에서는 항산화 활성, 미백, 콜라겐 생합성 증 진 효과와 여드름균인 P. acenes에 대해서도 항균 활성을 나 타내었으며, 이러한 효능은 추출물에 함유되어 있는 다량의 페놀성 화합물로부터 기인한 것으로 판단되고 있다 [4]. 하 지만, 사과 캘러스 관련 연구는 스위스 사과 품종인 'Uttwiler Sptlauber' 캘러스 추출물의 Human stem cell 증식 및 보호 효 과, 모낭세포의 노화 억제, 임상에서의 눈가의 주름개선 효 과가 알려져 있을 뿐 다른 효능에 관한 연구는 미비한 실정 이다 [5].…”

Effect of vacuum blending on antioxidant activities of apple juice and blueberry juice

“…Rapid development of living standards has resulted in increased awareness of health and well-being, with a focus on the physiological effects in the human body, such as disease prevention and suppression of aging (Choi et al 2011). Free radicals, which are always generated by oxidative stress, cause various diseases including aging and cancer by putting stress on physiological functions in the body, thus interest in antioxidant nutrients is increasing (Shulz 1994).…”