Screening of exopolysaccharide-producing coccal lactic acid bacteria isolated from camel milk and red meat of Algeria

Kersani, ImÃ ̈ne; Zadi‐Karam, Halima; Karam, Nour-Eddine

doi:10.5897/ajb2017.15907

Cited by 7 publications

(3 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…EPS production was evaluated with ruthenium red staining method in ruthenium milk agar (0.5% yeast extract, 10% skim milk powder, 1% sucrose, 1.6% agar, 0.08 g/L ruthenium red) [20]. The coagulated cultures were considered as EPS-positive when a white loop was formed after incubation at 37 • C for 24 h. The analysis was conducted in triplicate.…”

Section: Exopolysaccharide Production (Eps)mentioning

confidence: 99%

Enterococci Isolated from Cypriot Green Table Olives as a New Source of Technological and Probiotic Properties

2018

View full text Add to dashboard Cite

Table olive is one of the main fermented vegetable worldwide and can be processed as treated or natural product. Lactic Acid Bacteria (LAB) are responsible for the fermentation of treated olives. The aim of this work was to study the technological characteristics and the potential probiotic properties of LAB isolated from Cypriot green table olives. This is the first comprehensive report on the isolation and characterization of LAB isolates retrieved from Cypriot green table olives. From a collection of 92 isolates from spontaneously fermenting green olives, 64 g positive isolates were firstly identified to genus level using biochemical tests, and secondly to species level using multiplex species specific polymerase chain reaction (PCR) amplifications of the sodA gene. Moreover, each of our isolates were tested for their technological and probiotics properties, as well as for their safety characteristics, using biochemical and molecular methods, in order to be used as starter cultures. Finally, to discriminate the most promising isolates on the base of their technological and probiotics properties, Principal component analysis was used. All the isolates were identified as Enteroccocus faecium, having interesting technological properties, while pathogenicity determinants were absent. Principal component analysis showed that some isolates had a combination of the tested parameters. These findings demonstrate that enteroccoci from Cypriot table olives should be considered as a new source of potential starter cultures for fermented products, having possibly promising technological and probiotic attributes.

show abstract

Section: Exopolysaccharide Production (Eps)mentioning

confidence: 99%

Enterococci Isolated from Cypriot Green Table Olives as a New Source of Technological and Probiotic Properties

2018

View full text Add to dashboard Cite

show abstract

“…Overnight cultures of isolates were spotted (5.0 µL) on the surface of MRS plates supplemented with 0.08 g/L of ruthenium red (Sigma-Aldrich). After incubation at 37°C for 48 h, exopolysaccharide (EPS) producing strains gave white colonies, while non-producers appeared as red colonies (Kersani et al 2017).…”

Section: Exopolysaccharide Productionmentioning

confidence: 99%

Investigating the biotechnological potential of lactic acid bacteria strains isolated from different Algerian dairy and farm sources

et al. 2022

View full text Add to dashboard Cite

Currently, Consumption of spontaneously fermented milks is common in Algeria, making it a feasible source of diverse lactic acid bacteria (LAB) with the potential to be used as adjunct cultures to improve quality and safety of fermented dairy products. In this context, to select eligible indigenous strains which could be applied as bioprotective and/or starter cultures, the present study aimed to characterize the genomic variability, biotechnological potential and safety of thirty-eight LAB isolated from Algerian dairy and farm sources of western Algeria. The isolates were unequivocally identified by 16S rRNA gene and fingerprint-based methods. The following species were identified: Enterococcus faecium (n=15), Enterococcus durans (n=2), Enterococcus hirae (n=2), Enterococcus lactis (n=1), Lactiplantibacillus plantarum (n=6), Lactococcus lactis (n=4), Levilactobacillus brevis (n=3), Lacticaseibacillus paracasei (n=3), Lacticaseibacillus rhamnosus (n=1), and Pediococcus acidilactici (n=1). Among the strains, three of them, L. lactis LGMY8, Lb. plantarum LGMY30 and Lb. paracaseiLGMY31 were safe and showed some valuable biotechnological properties, such as high acidification, proteolytic activity, EPS production, and inhibition of undesirable bacteria, that made them powerful candidates to be used as starter.

show abstract

“…All plates were incubated at 37°C for 72 hours, and morphologically distinct colonies that grew on the plate were selected and purified via successive streak plating using the same solid media. EPS-producing bacteria were first selected on the basis of the mucoid appearance and ropiness by stroking them with sterile inoculating loop according to the method of Kersani et al (2017). Exopolysaccharide production of the isolates was further assessed by inoculation onto Congo red agar medium and incubation at 37°C for 48 hours.…”

Section: Isolation and Screening Of Isolates For Eps Productionmentioning

confidence: 99%

Bacterial Exopolysaccharide-Mediated Synthesis of Silver Nanoparticles and Their Application on Bacterial Biofilms

Galvez¹,

Ramos²,

Teja³

et al. 2019

J microb biotech food sci

View full text Add to dashboard Cite

The development of multi-drug-resistant bacteria and their biofilm formation capabilities has decreased the effectiveness of antibiotics and other antimicrobial agents. Silver nanoparticle (AgNP) is currently being explored as an alternative strategy in countering biofilm-related infections. In this study, AgNP was biosynthesized using the extracted exopolysaccharide (EPS) from two obligate alkaliphilic bacteria, Bacillus krulwichiae M2.5 and Bacillus cellulosilyticus M4.1, isolated from a hyperalkaline spring in Zambales, Philippines. The samples exhibited an absorption peak at around 420 nm corresponding to the surface plasmon resonance of AgNPs as shown by UV-Vis analysis. The Fourier transform infrared (FTIR) spectroscopy spectra revealed functional groups in the EPS such as hydroxyl and carboxyl involved in the reduction of Ag+ to AgNP. Scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDX) spectroscopy showed stable irregular and spherical AgNPs with an average size of 25.88 ± 10.49 nm and 23.99± 8.43 nm for B. krulwichiae M2.5 EPS-AgNP and B. cellulosilyticus M4.1 EPS-AgNP, respectively. The biosynthesized AgNPs significantly inhibited the biofilm formation of Pseudomonas aeruginosa, Klebsiella pneumoniae and Staphylococcus aureus in a dose-dependent manner as determined by microtiter plate assay. However, the results showed a reduced inhibitory effect of the synthesized AgNPs on established biofilms indicating the need for higher AgNP concentration. This study demonstrates that EPS from bacteria adapted to alkaline conditions can be used for efficient AgNP biosynthesis with potential biomedical applications.

show abstract

Screening of exopolysaccharide-producing coccal lactic acid bacteria isolated from camel milk and red meat of Algeria

Cited by 7 publications

References 60 publications

Enterococci Isolated from Cypriot Green Table Olives as a New Source of Technological and Probiotic Properties

Enterococci Isolated from Cypriot Green Table Olives as a New Source of Technological and Probiotic Properties

Investigating the biotechnological potential of lactic acid bacteria strains isolated from different Algerian dairy and farm sources

Bacterial Exopolysaccharide-Mediated Synthesis of Silver Nanoparticles and Their Application on Bacterial Biofilms

Contact Info

Product

Resources

About