Many studies have shown that Lactobacillus species are bacteria that are mostly used as probiotics.Here, we evaluated cholesterol assimilation and folic acid production by determining the probiotic properties of Lactobacillus spp from raw goat milk, which has prebiotic properties. Bacteria were isolated from goat milk, and API 50 CHL and 16sRNA identi cation was performed. Probiotic properties were determined based on bile salt and acidic tolerance, hydrophobicity, haemolytic activity, antibiotic susceptibility, antagonistic effect, and exopolysaccharide production. In addition, cholesterol assimilation and folate production of cultures were determined. The exopolysaccharide production of Lactobacillus plantarum GM-12 was moderate (++), with the highest folate production (179 ng /dl), and high cholesterol assimilation (60 mg / dl). The exopolysaccharide production of L. plantarum GM-15 was high (+++), with high folate production (160 ng / dl) and high cholesterol assimilation (58 mg / dl). It is understood that these two strains may be potential probiotics and At the same time, these two lactobacilli are strong candidates for use as starter cultures.