Screening of endophytic fungi as potential antagonistic agents of Pyricularia oryzae and evaluation of their ability in producing hydrolytic enzymes

Putri, Novia Dwi; Sulistyowati, Liliek; Aini, Luqman Qurata; Muhibuddin, Anton; TRIANTI, IRISA

doi:10.13057/biodiv/d230248

Cited by 12 publications

(7 citation statements)

References 38 publications

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“…Previous studies indicating that chitinases are involved in triggering a plant's defense mechanisms corroborate the current results (Baek et al, 1999;Gueye et al, 2020). The current results are also supported by studies that have found that the hydrolytic enzymes (cellulase, chitinase, protease, and b-(1,3)-glucanases) produced by T. asperellum, T. harzianum, and T. longibrachiatum have a high level of antagonistic activity against F. oxysporum (Schirmböck et al, 1994;Putri et al, 2022). These enzymes play a key role in the breakdown of the pathogen's cell walls, which facilitates simple invasion and colonization by the biocontrol agents, induces a defense response and slows the spread of the pathogen while also indirectly promoting plant growth and development (Qualhato et al, 2013;Cherkupally et al, 2017).…”

Section: Discussionsupporting

confidence: 89%

“…To induce enzyme production, basal salts of the following compositions were added: MgSO 4 .7H 2 O (0.3 g), (NH 4 ) 2 SO 4 (3 g), KH 2 PO 4 (2 g), agar (15 g). They were augmented with 1% (w/v) colloidal chitin (Rojas-Avelizapa et al, 1999;El-Sobky et al, 2019), 1% (w/v) CMC, 1% (w/v) gelatin, and 2.0% (w/v) laminarin), to detect the enzymes chitinase, cellulase, protease and b-(1-3) glucanase (Nagpure et al, 2014;Putri et al, 2022), respectively. The incubation was continued for 24 h, and the culture filtrate separated by filtration and centrifugation (at 5000 rpm, 4°C, for 10 min) and used as the enzyme source (Maria et al, 2005;Agrawal and Kotasthane, 2012;Saravanakumar et al, 2017).…”

Section: Biocontrol-related Enzymesmentioning

confidence: 99%

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Biological management of coffee wilt disease (Fusarium xylarioides) using antagonistic Trichoderma isolates

et al. 2023

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Coffee wilt disease (CWD) is a serious threat to the food security of small-scale farmers in Ethiopia, causing significant reductions in coffee yield. Currently, there are no effective control measures available against the causative agent of CWD, Fusarium xylarioides. The main objective of this study was therefore to develop, formulate, and evaluate a range of biofungicides against F. xylarioides, derived from Trichoderma species and tested under in vitro, greenhouse, and field conditions. In total, 175 Trichoderma isolates were screened as microbial biocontrol agents against F. xylarioides. The efficacy of two biofungicide formulations, wettable powder and water dispensable granules, were tested on the susceptible Geisha coffee variety in three different agro-ecological zones in southwestern Ethiopia over three years. The greenhouse experiments were set up using a complete block design, while in the field a randomized complete block design was used, with twice yearly applications of biofungicide. The test pathogen spore suspension was applied to the coffee seedlings by soil drenching, and the subsequent incidence and severity of CWD evaluated annually. The mycelial growth inhibition profiles of the Trichoderma isolates against F. xylarioides ranged from 44.5% to 84.8%. In vitro experiments revealed that T. asperelloides AU71, T. asperellum AU131 and T. longibrachiatum AU158 reduced the mycelial growth of F. xylarioides by over 80%. The greenhouse study indicated that wettable powder (WP) of T. asperellum AU131 had the highest biocontrol efficacy (84.3%), followed by T. longibrachiatum AU158 (77.9%) and T. asperelloides AU71 (71.2%); they also had a significant positive impact on plant growth. The pathogen-treated control plants had a disease severity index of 100% across all the field experiments, and of 76.7% in the greenhouse experiments. In comparison to untreated controls, the annual and cumulative disease incidence over the three years of the study period varied from 46.2 to 90%, 51.6 to 84.5%, and 58.2 to 91%, at the Teppi, Gera and Jimma field experimental locations. Overall, the greenhouse and field experiments and in vitro assays support the biocontrol potential of Trichoderma isolates, and T. asperellum AU131 and T. longibrachiatum AU158 in particular are recommended for the management of CWD under field conditions.

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Section: Discussionsupporting

confidence: 89%

Section: Biocontrol-related Enzymesmentioning

confidence: 99%

Biological management of coffee wilt disease (Fusarium xylarioides) using antagonistic Trichoderma isolates

et al. 2023

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“…6). Chitinase plays a key role in hyphal/mycelium development and branching, spore production and germination, fungal cell wall degradation or morphogenesis of fungal cell wall structure [46]. This key role defines the role of B. cereus SAHA 12.13 chitinase enzyme in inhibiting pathogenic fungi, namely inhibiting spore production and germination.…”

Section: Antagonistic Activity Of B Cereus Saha 1213 Isolates Against...mentioning

confidence: 99%

Characterization of Extracellular Chitinase from Bacillus cereus SAHA 12.13 and Its Potency as a Biocontrol of Curvularia affinis

Asril,

Supriyadi

2024

J. Multidiscip. Appl. Nat. Sci.

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Bacillus cereus SAHA 12.13 can produce chitinase, an enzyme that digests chitin in the main compounds of cell walls, mycelia, and spores in pathogenic fungi that cause leaf spots on oil palm plants such as Curvularia affinis. This study aims to determine the properties of the chitinase enzyme B. cereus SAHA 12.13 that can inhibit the growth of C. affinis. Chitinase enzyme production and characterization were measured using the Spindler method. Antagonism test against pathogenic fungi using dual culture method by testing cell culture and enzyme crude extract. This result showed that the isolate produced a high level of specific chitinase activity at 37 °C for 45 h of incubation with 8.45 U mg-1 proteins with a growth rate (k) of 0.25 generation/h, and the generation time was 3.96 h/generation. The optimum chitinase activity was achieved at pH 7.0 and 45 °C and was stable for 3 h with a half-life (t1/2) of 770 min. The crude enzyme and cell culture of strain can inhibit the growth of C. affinis by 36.27±0.043% and 34.25±0.041%, respectively. These characteristics indicate that B. cereus strain SAHA12.13 can be used to inhibit C. affinis, which causes leaf blight of oil palm, under varying pH and temperature conditions.

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“…The PCR cycles included 3 minutes of pre-denaturation at 95°C, 30 seconds of denaturation at 95°C, 30 seconds of annealing at 50°C, 1 minute of extension at 72°C, 1 minute of final extension at 72°C. PCR products were analyzed by electrophoresis in 1% agarose gel and treated with 0.75 l DNA dye (Nandika et al 2021;Putri et al 2022). DNA double helix sequences were assembled and analyzed using Genetyx (version 11.0) and Genetyx-ATSQ (version 4.0) software (Genetyx, Tokyo, Japan) sequentially and compared with same DNA sequences accessed from DDBJ/ EMBL/ GenBank via NCBI BLAST program (Parwanayoni 2018).…”

Section: Molecular Identificationmentioning

confidence: 99%

Short Communication: First report of Nectria haematococca causing a moler disease on shallots in West Nusa Tenggara, Indonesia

et al. 2022

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Abstract. Mariani, Suprapta DN, Sudana IM, Temaja IGRM, Sudantha IM. 2022. Short Communication: First report of Nectria haematococca causing a moler disease on shallots in West Nusa Tenggara, Indonesia. Biodiversitas 23: 2768-2774. Moler disease caused by Nectria haematococca is the main disease of shallots in West Nusa Tenggara, Indonesia. Moler disease caused significant yield loss and even crop failure. The purpose of this study was to isolate and identify the pathogen causing moler disease on shallots. This research was conducted from February 2018 to November 2019. Survey was conducted at 13 shallot planting locations in West Nusa Tenggara, namely: Ngali, Ncera, Cenggu, Renda, Sajang, Rensing, Dasan Baru, Batu Layar, Anyar, Sembalun, Jerowaru, Batujai and Sakuru Village. Ten infected plants were taken, at each observation point, then put in a labeled plastic bag and brought to the laboratory for isolation purposes. The results showed that pathogen causing moler disease on shallot was isolated NTB2018. On PDA isolate NTB2018 produced white color colony, the mycelium growth direction was sideways with smooth mycelium structure, sideways growth direction of mycelium and fine structure mycelium. The fungus produced insulated mycelium consisting of macroconidia and microconidia as asexual spores and ascospores as sexual spores. Based on analysis of 18S rRNA gene, isolate NTB2018 DNA fragment of ±569 bp.

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Screening of endophytic fungi as potential antagonistic agents of Pyricularia oryzae and evaluation of their ability in producing hydrolytic enzymes

Cited by 12 publications

References 38 publications

Biological management of coffee wilt disease (Fusarium xylarioides) using antagonistic Trichoderma isolates

Biological management of coffee wilt disease (Fusarium xylarioides) using antagonistic Trichoderma isolates

Characterization of Extracellular Chitinase from Bacillus cereus SAHA 12.13 and Its Potency as a Biocontrol of Curvularia affinis

Short Communication: First report of Nectria haematococca causing a moler disease on shallots in West Nusa Tenggara, Indonesia

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