Screening of Endophytes from Traditionally Used Medicinal Plants of Manipur for Their Antimicrobial Activity An Impact towards Future Drug Discovery

Ngashangva, Ng; S, Indira Devi; Kalita, Mohan Chandra

doi:10.26438/ijsrbs/v6i5.3947

Cited by 2 publications

(9 citation statements)

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“…A gram-positive bacterium, P. peoriae IBSD35, isolated from the stem of M. pachycarpa Benth. retained antimicrobial activity against S. aureus ATCC 25923 ( DeFilipps and Krupnick, 2018 ; Ngashangva et al, 2019 ). Its optimum growth condition was observed at pH∼6.8 at 38°C in LB and BHI media.…”

Section: Discussionmentioning

confidence: 99%

“…It was observed that high salt concentration impeded the further purification process. The enriched protein complex is purified with positively charged resin DEAE-C based on the biomolecule physiochemical characteristics and RP-HPLC ( Conlon, 2006 ; Ngashangva et al, 2019 ). The PR-HPLC purified sample was analyzed with LC-MS/MS ( Eng et al, 1994 ; Dancík et al, 1999 ).…”

Section: Discussionmentioning

confidence: 99%

“…( Ito et al, 2006 ; Srivastava, 2010 ) was revived from the stock (Sanyo Biomedical Freezer) preserved in the Microbial Repository Centre of IBSD (Accession No. MRC-75001 ) ( Ngashangva et al, 2019 ). It was streaked on a Luria Bertani (LB) agar plate and incubated in an Eppendorf innova R 42 Rotary shaker at 38°C for 12 h to check the colony purity and contamination.…”

Section: Methodsmentioning

confidence: 99%

“…The sample was loaded on a semi-preparative Agilent ZORBAX 300SB reverse-phase C-18 of 5 μm and a 9.4 X 250 mm column and eluted out with 0.1% ion pairing reagent and trifluoroacetic acid (TFA) (HPLC grade) ( Conlon, 2006 ). The peaks were pooled together and lyophilized in a Modulyod Freeze Dryer (Thermo) to form powder (35XT) ( Ngashangva et al, 2019 ). The retention of antimicrobial activity was tested at each step of purification ( Bradford, 1976 ; Magaldi et al, 2004 ; CLSI, 2008 ).…”

Section: Methodsmentioning

confidence: 99%

“…An endophytic gram-positive bacterium, Paenibacillus peoriae IBSD35 was isolated from Millettia pachycarpa Benth. in our previous experiment using the standardized surface sterilization method ( Srivastava, 2010 ; Ngashangva et al, 2019 ). Novel antimicrobial peptides (AMP) discovery requires the materials of ethnopharmacology, herbal medicines, and traditional knowledge systems to facilitate the process ( Mukherjee, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Antimicrobial Peptide Metabolome of Bacterial Endophyte Isolated From Traditionally Used Medicinal Plant Millettia pachycarpa Benth

et al. 2021

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Increasing prevalence of antimicrobial resistance (AMR) has posed a major health concern worldwide, and the addition of new antimicrobial agents is diminishing due to overexploitation of plants and microbial resources. Inevitably, alternative sources and new strategies are needed to find novel biomolecules to counter AMR and pandemic circumstances. The association of plants with microorganisms is one basic natural interaction that involves the exchange of biomolecules. Such a symbiotic relationship might affect the respective bio-chemical properties and production of secondary metabolites in the host and microbes. Furthermore, the discovery of taxol and taxane from an endophytic fungus, Taxomyces andreanae from Taxus wallachiana, has stimulated much research on endophytes from medicinal plants. A gram-positive endophytic bacterium, Paenibacillus peoriae IBSD35, was isolated from the stem of Millettia pachycarpa Benth. It is a rod-shaped, motile, gram-positive, and endospore-forming bacteria. It is neutralophilic as per Joint Genome Institute’s (JGI) IMG system analysis. The plant was selected based on its ethnobotany history of traditional uses and highly insecticidal properties. Bioactive molecules were purified from P. peoriae IBSD35 culture broth using 70% ammonium sulfate and column chromatography techniques. The biomolecule was enriched to 151.72-fold and the yield percentage was 0.05. Peoriaerin II, a highly potent and broad-spectrum antimicrobial peptide against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Candida albicans ATCC 10231 was isolated. LC-MS sequencing revealed that its N-terminal is methionine. It has four negatively charged residues (Asp + Glu) and a total number of two positively charged residues (Arg + Lys). Its molecular weight is 4,685.13 Da. It is linked to an LC-MS/MS inferred biosynthetic gene cluster with accession number A0A2S6P0H9, and blastp has shown it is 82.4% similar to fusaricidin synthetase of Paenibacillus polymyxa SC2. The 3D structure conformation of the BGC and AMP were predicted using SWISS MODEL homology modeling. Therefore, combining both genomic and proteomic results obtained from P. peoriae IBSD35, associated with M. pachycarpa Benth., will substantially increase the understanding of antimicrobial peptides and assist to uncover novel biological agents.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Analysis of Antimicrobial Peptide Metabolome of Bacterial Endophyte Isolated From Traditionally Used Medicinal Plant Millettia pachycarpa Benth

et al. 2021

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Integrated genomics and proteomics analysis of Paenibacillus peoriae IBSD35 and insights into its antimicrobial characteristics

Ngashangva

Sharma

Kalita

et al. 2022

Sci Rep

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Antimicrobial resistance has been developing fast and incurring a loss of human life, and there is a need for new antimicrobial agents. Naturally occurring antimicrobial peptides offer the characteristics to counter AMR because the resistance development is low or no resistance. Antimicrobial peptides from Paenibacillus peoriae IBSD35 cell-free supernatant were salted out and purified using chromatography and characterized with liquid chromatography–tandem-mass spectrometry. The extract has shown a high and broad spectrum of antimicrobial activity. Combining the strain IBSD35 genome sequence with its proteomic data enabled the prediction of biosynthetic gene clusters by connecting the peptide from LC–MS/MS data to the gene that encode. Antimicrobial peptide databases offered a platform for the effective search, prediction, and design of AMPs and expanded the studies on their isolation, structure elucidation, biological evaluation, and pathway engineering. The genome-based taxonomy and comparisons have shown that P. peoriae IBSD35 is closely related to Paenibacillus peoriae FSL J3-0120. P. peoriae IBSD35 harbored endophytic trait genes and nonribosomal peptide synthases biosynthetic gene clusters. The comparative genomics revealed evolutionary insights and facilitated the discovery of novel SMs using proteomics from the extract of P. peoriae IBSD35. It will increase the potential to find novel bio-molecules to counter AMR.

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Screening of Endophytes from Traditionally Used Medicinal Plants of Manipur for Their Antimicrobial Activity An Impact towards Future Drug Discovery

Cited by 2 publications

References 29 publications

Analysis of Antimicrobial Peptide Metabolome of Bacterial Endophyte Isolated From Traditionally Used Medicinal Plant Millettia pachycarpa Benth

Analysis of Antimicrobial Peptide Metabolome of Bacterial Endophyte Isolated From Traditionally Used Medicinal Plant Millettia pachycarpa Benth

Integrated genomics and proteomics analysis of Paenibacillus peoriae IBSD35 and insights into its antimicrobial characteristics

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