Screening of Crude Plant Extracts with Anti-Obesity Activity

Abstract

Obesity is a global health problem. It is also known to be a risk factor for the development of metabolic disorders, type 2 diabetes, systemic hypertension, cardiovascular disease, dyslipidemia, and atherosclerosis. In this study, we screened crude extracts from 400 plants to test their anti-obesity activity using porcine pancreatic lipase assay (PPL; triacylglycerol lipase, EC 3.1.1.3) in vitro activity. Among the 400 plants species examined, 44 extracts from plants, showed high anti-lipase activity using 2,4-dinitrophenylbutyrate as a substrate in porcine pancreatic lipase assay. Furthermore, 44 plant extracts were investigated for their inhibition of lipid accumulation in 3T3-L1 cells. Among these 44 extracts examined, crude extracts from 4 natural plant species were active. Salicis Radicis Cortex had the highest fat inhibitory activity, whereas Rubi Fructus, Corni Fructus, and Geranium nepalense exhibited fat inhibitory capacity higher than 30% at 100 μg/mL in 3T3-L1 adipocytes, suggesting anti-obesity activity. These results suggest that four potent plant extracts might be of therapeutic interest with respect to the treatment of obesity.

Antiobesity activity of Moringa oleifera leaves against high fat diet-induced obesity in rats

“…1 saturated fat, high calorie content), and sedentary lifestyles. 2 Individual is considered obese when the amount of fat tissue is increased to such an extent that physical and mental health are affected and life expectancy reduced.…”

Chemical composition profile of the essential oil fromhymenocrater bituminousand its health functionality

“…[21] Also, porcine pancreatic lipase (type-II) inhibitory activity of the samples was determined using p-nitrophenyl butyrate (p-NPB) as substrate. [27] In brief, enzyme solution (1 mg/mL) was prepared in 50 mM Tris-HCl (pH 8.0). Test solution (25 µL) was mixed with lipase solution (50 µL) in a 96-well microplate and incubated for 20 min at 25°C.…”

Saponin-enriched sea cucumber extracts exhibit an antiobesity effect through inhibition of pancreatic lipase activity and upregulation of LXR-β signaling

“…The success of orlistat encouraged the development of PL inhibitors as weight-reducing drugs. In new weight-reducing therapies with more potency or fewer side effects, several natural PL inhibitors, such as platycodin saponins, scabiosaponins, chikusetsusaponins, tea saponins, assamicain A, oolonghomobisflavan A, and oolonghomobisflavan B, have been identified as inhibitors of PL activity in vitro (de la Garza et al, 2011;Han et al, 1999Han et al, ,2005Roh & Jung, 2012;Slanc et al, 2009;Zhao & Kim, 2004;Zheng et al, 2004). However, the compounds are difficult to develop as antiobesity drugs because of limited sources.…”