Screening of Burkholderia spp. from oil palm plantation with antagonistic properties against Ganoderma boninense

Abstract

Abstract. Yurnaliza. Rambe DI, Sarimunggu L, Purba M, Nurwahyuni I, Lenny S, Lutfia A, Hartanto A. 2020. Screening of Burkholderia spp. from oil palm plantation with antagonistic properties against Ganoderma boninense. Biodiversitas 21: 3431-3437. Burkholderia spp. are ubiquitous diazotrophic bacteria within β-Proteobacteria group, known for its occurrence in variety of niches from aquatic to terrestrial habitats and as endophytes. Beneficial strains of Burkholderia have been reported as plant growth-promoting rhizobacteria or as antagonistic bacteria against phytopathogenic fungi. This study evaluated the potential of multiple strains of Burkholderia spp. recovered from three ecological niches, such as rhizosphere, humus soil, and root endophytes of oil palm plantations in North Sumatra to suppress the growth of basal stem rot causative agent in oil palm (Elaeis guineensis Jacq.) by Ganoderma boninense Pat. The antagonistic isolates were identified on the basis of molecular identification using 16S rDNA sequence (27F–1462R), revealing twelve isolates (48%) as members of Burkholderia cepacia complex (Bcc), with other minor genera, such as Chitinophaga, Klebsiella, Mycobacterium, Paenibacillus, Rhizobium, Serratia, Stenotrophomonas, and Xanthomonas. The antagonistic activities as expressed in the percentage inhibition of radial growth (PIRG) against G. boninense were considerably potential with the highest percentage of 55%. In comparison, the crude extract (MeOH, EtOAc) was also tested against G. boninense colonies showing PIRG from 0 to 38%. Majority of isolates did not show any visible chitinolytic activity based on plate assay, in exception to B. contaminans RC02 while most of them were producers of glucanase. The collection of indigenous Burkholderia spp. originating from North Sumatran oil palm plantations, i.e. B. cepacia, B. contaminans, B. metallica, and B. stagnalis may then be considered as potential biocontrol agents against G. boninense based on their antagonistic activities, antifungal properties, and hydrolytic enzyme activities.

Induction of Resistance of Antagonistic Bacterium Burkholderia contaminans to Postharvest Botrytis cinerea in Rosa vinifera

“…In order to better ensure the quality of grape products, there are anticorrosion technologies in biological control technology, which can improve the resistance to pathogens. The fruit postharvest resistance elicitor can effectively resist pathogens and reduce the degree of decay by stimulating plant disease resistance [ 1 ]. In this paper, the antagonistic Burkholderia contaminans was used as the biocontrol strain to study its induction mechanism of postharvest disease resistance of grapes, so as to provide reference basis for the development of postharvest biological control technology of grapes [ 2 ].…”

In vitro antagonistic activity of soil microbes isolated from oil palm to Ganoderma zonatum

“…Genus Burkholderia is a potential biocontrol agent in agriculture. Several strains of Burkholderia are antagonistic against phytopathogenic fungi, besides plant growth-promoting rhizobacteria [14]. The genus Burkholderia is common as soil inhabitants, distributed in soil pH 9-12, and potential as a biological control agent in agriculture [15].…”

An In-Depth Study of Phytopathogenic Ganoderma: Pathogenicity, Advanced Detection Techniques, Control Strategies, and Sustainable Management

“…strains recovered from oil palm plantations showed significant antagonistic activities against G. boninense . These strains, including B. cepacia , B. contaminans , B. metallica , and B. stagnalis , exhibit potential as biocontrol agents based on their antifungal properties and hydrolytic enzyme activities [ 330 ]. Scytalidium parasiticum shows potential as a biological control agent against Ganoderma .…”