Screening of Bioactive Compounds of Medicinal Mushrooms Collected on Tunisian Territory

Abstract

This study is, to our knowledge, the first to investigate the pharmacological importance of wild Tunisian mushrooms. Ethanolic extracts of 5 Tunisian mushrooms-Phellinus torulosus, Fomes fomentarius, Trametes versicolor, Pisolithus albus, and Fomitopsis pinicola-were collected from the Kroumirie Region (North Tunisia). The dry basidomes of mushrooms were extracted using ethanol and evaluated for total polyphenol, flavonoid, flavonol, tannin, proanthocyanidin, and anthocyanin content. In addition, their antioxidant activities were determined using 3 assays (testing 2,2-diphenyl-1-picrylhydrazyl [DPPH] radical scavenging, the reducing power of iron, and the iron-chelating power). Their antimicrobial activities were assessed against 8 bacterial species. The results revealed the presence of significant differences between the secondary metabolites and biological activities of the different tested extracts. In addition, significant correlations were observed between antioxidant activities and phenolic contents. Crude ethanol extracts prepared from basidomes of F. fomentarius and Ph. torulosus have higher total phenolic content and antioxidant activity per the DPPH and metal-chelating activity assays. The reducing power assay showed that the ethanolic extract of F. pinicola had the highest activity. Ethanolic extracts of the 5 mushrooms have antibacterial activity against the evaluated strains.

Evaluation of phenolic profile, antioxidant and anticholinesterase effects of Fuscoporia torulosa

“…The methanol extract with higher concentrations of phenolic contents showed the highest activity in all antioxidant activity assays except metal chelating assay. Szychowski et al [22] [29] studied antioxidant activity of the ethanol extract of F. torulosa by using DPPH • radical scavenging, the reducing power of iron and the iron-chelating power assays and the ethanol extract showed high antioxidant activity in DPPH • and iron-chelating assays. In a different study, DPPH • radical scavenging activities of the hexane, chloroform, 50% methanol and water extracts of F. torulosa were determined and the water extract indicated higher antioxidant activity among the other extracts [30].…”

Antioxidant potential of extracts of three mushroom species collected from the Republic of North Macedonia

“…Moreover, Khadhri et al. (2017) found that the ethanolic extract of Trametes versicolor contained 60.7 mg of GAE/g polyphenols, which is a higher value compared to the ethanolic extract of Phellinus torulosus where the polyphenol content is 33.1 mg GAE/g. The flavonoid content in the ethanolic extract of Trametes versicolor was 8.4 mg QE/g, which is a little higher compared to the same extract in this research.…”

“…According to Khadhri et al. (2017) in terms of the ability to capture free DPPH radicals, the ethanolic extract of Trametes versicolor has an IC 50 value of 0.12 mg/ml, and the ethanolic extract of Phellinus torulosus has an IC 50 value of 0.025 mg/ml.…”

Triterpenes and Phenolic Compounds From the Fungus <em>Fuscoporia torulosa</em>: Isolation, Structure Determination and Biological Activity

“…The fungal metabolites were examined for their potential cytotoxic, antioxidant, cholinesterase, and tyrosinase inhibitory activities (Deveci et al). Besides these mycochemical studies several articles reported on the antimicrobial, antioxidant, cytotoxic and xanthine oxidase inhibitory, and anti-acne activities and the phenolic profile of F. torulosa samples prepared with solvents of different polarities [2,[12][13][14].…”