Screening of bioactive compounds in truffles and evaluation of pressurized liquid extractions (PLE) to obtain fractions with biological activities

Chemical Screening of Metabolites Profile from Romanian Tuber spp.

“…The spectra disclose a very complex mixture of molecules from which only some molecules were detected. A total of 109 identified metabolites were attributed to different chemical classes such as amino acids, saccharides, flavonoids, aldehyde, ketone, esters, sulfur compounds, terpenoids, phenolic acids, steroids, hydrocarbons, and other data confirming results already published in the literature [7,10,15,17,[19][20][21][22][23][24][25][26][27][28][29]. The results of the GC-MS were confirmed by ESI-QTOF-MS analysis.…”

“…Recently, special attention was given to the potential biomedical application of hypogean fungus bioactive compounds, in particular, phytosterols, fatty acids, phenols, amino acids, volatile components, etc. [1,3,[6][7][8]11,[15][16][17][18][19][20][21][22][23][24][25][26][27][28][29]. However, still only a few scientific studies have been undertaken on secondary metabolites with therapeutic potential and on truffles' biology [3,6,7,[19][20][21][22][23][24][25][26][27][28][29].…”

Application of Pressurized Liquid Extractions to Obtain Bioactive Compounds from Tuber aestivum and Terfezia claveryi

“…The main nutritional constituents of truffles are carbohydrates, followed by proteins [3][4][5]. Most of their carbohydrates are considered dietary fibers such as chitin, β-glucans and other polysaccharides, and they also include mannitol and trehalose [6] as well as smaller sugars such as D-glucose, D-mannose or D-galactose [7]. Although truffles show low fat levels, their lipid content is important since they are involved in flavor and aroma properties.…”

“…Fungal sterol-enriched fractions also reported some bioactivity such as hypocholesterolemic [14,15]. Recently, truffles have shown interesting bioactive compounds, and their potential bioactivities are now being studied, e.g., antitumoral, antioxidant, immunomodulatory and hypoglucemic properties [7,[16][17][18][19].…”

“…In comparison with conventional extraction techniques (soxlhet, maceration, solvent extraction), PLE involves some advantages such as: time saving, less solvent consumption, and use of nontoxic solvents (water or ethanol), among others [22]. However, studies to optimize PLE methods to extract bioactive ingredients from truffles are scarce, particularly from those of desert truffles [7,23]. Truffles are usually consumed fresh and during special occasions; however, with the improvements implemented during their cultivation, truffle yields are exponentially increasing and becoming more and more accessible.…”