Screening of bacterial DNA in bile sampled from healthy dogs and dogs suffering from liver- or gallbladder-associated disease

Abstract

Although the biliary system is generally aseptic, gallbladder microbiota has been reported in humans and some animals apart from dogs. We screened and analyzed the bacterial deoxyribonucleic acid in canine gallbladders using bile sampled from 7 healthy dogs and 52 dogs with liver-or gallbladder-associated disease. PCR screening detected bacteria in 17.3% of diseased dogs (9/52) and none in healthy dogs. Microbiota analysis of PCRpositive samples showed that the microbial diversity differed between liver-and gallbladder-associated disease groups. Thus, a specific bacterial community appears to occur at a certain frequency in the bile of diseased dogs.

Metataxonomic Analysis of the Uterine Microbiota Associated with Low Fertility in Dairy Cows Using Endometrial Tissues Prior to First Artificial Insemination

“…The DNA solutions were examined using a spectrophotometer (NanoDrop 1000; Thermo Scientific, Wilmington, DE, USA) to measure the DNA quality and amount. To determine the study enrollment, the presence and quality of bacterial DNA were then examined by PCR targeting the 16S rRNA gene, as described previously ( 58 ).…”

Metataxonomic analysis of the uterine microbiota associated with low fertility in dairy cows using endometrial tissues prior to first artificial insemination

“…The bacterial DNA in the samples was veri ed by PCR, as described elsewhere. The presence of bacterial DNA was examined using PCR targeting the 16S rRNA gene, as described elsewhere [55].…”