Metataxonomic Analysis of the Uterine Microbiota Associated with Low Fertility in Dairy Cows Using Endometrial Tissues Prior to First Artificial Insemination

Yagisawa, Takuya; Uchiyama, Jumpei; Takemura‐Uchiyama, Iyo; Ando, Shun; Masum, Md. Abdul; Murakami, Hironobu; Matsushita, Osamu; Katagiri, Seiji

doi:10.1128/spectrum.04764-22

Cited by 4 publications

(3 citation statements)

References 64 publications

(87 reference statements)

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“…Open cows also harbored a greater relative abundance of ASVs identified as the Rikenellaceae RC9 gut group (ASV506) and Christensenellaceae R-7 group (ASV6112) in their uterus. Although both of these genera are part of the normal bovine gut microbiota ( 24 ), the Rikenellaceae RC9 gut group also colonizes the bovine uterus ( 18 ); however, there are no reports regarding their association with reproductive infections or fertility in cattle.…”

Section: Discussionmentioning

confidence: 99%

“…Likewise, the uterine microbiota of dairy cows diagnosed with clinical endometritis (CE) differed significantly from that of healthy cows and was characterized by reduced microbial diversity and an enrichment of Trueperella pyogenes ( 17 ). Besides the involvement of the uterine microbiome in uterine health, recent evidence suggests that dysbiosis of this microbiota prior to breeding may compromise host fertility ( 18 – 20 ). Thus, given the evidence from both humans and cattle suggesting a role for the vagino-uterine microbiomes in reproductive health and fertility, we hypothesize that fertility- related taxonomic signature may be present in the bovine vaginal and uterine microbiota.…”

Section: Introductionmentioning

confidence: 99%

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Sequencing and culture-based characterization of the vaginal and uterine microbiota in beef cattle that became pregnant or remained open following artificial insemination

Webb,

Holman,

Schmidt

et al. 2023

Microbiol Spectr

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In this study, we evaluated the vaginal and uterine microbiota between beef cattle that became pregnant via artificial insemination (AI) and those that remained open to identify microbial signatures associated with fertility. We also characterized the culturable fraction of these microbiota and screened certain vaginal and uterine bacterial isolates for antimicrobial resistance. For this, vaginal and uterine swabs were collected before AI from two cohorts of Angus-crossbred cattle: mature cows (vaginal and uterine: 27 open and 31 pregnant) and heifers (vaginal: 26 open and 33 pregnant). The microbiota of these samples were assessed using 16S rRNA gene sequencing and culturing. Twenty-nine vaginal and uterine bacterial isolates were screened for resistance against 41 antibiotics. Within the vaginal microbiota, 11 amplicon sequence variants (ASVs) were more relatively abundant in the open heifers compared with the pregnant heifers. No differentially abundant ASVs were detected in the vaginal samples from pregnant versus open cows. Pregnant cows had a distinct uterine microbiota community structure ( P = 0.008) and interaction network structure compared with open cows. Twenty-eight differentially abundant uterine ASVs were observed between the pregnant and open cows. Community structure and diversity were different between the cow’s vagina and uterus. A total of 733 bacterial isolates were recovered from vaginal (512) and uterine (221) swabs under aerobic (83 different species) and anaerobic (69 species) culturing. Among these isolates were pathogenic species and those mostly susceptible to tested antibiotics. Overall, our results indicate that fertility-associated taxonomic signatures are present in the bovine uterine and vaginal microbiota. IMPORTANCE Emerging evidence suggests that microbiome-targeted approaches may provide a novel opportunity to reduce the incidence of reproductive failures in cattle. To develop such microbiome-based strategies, one of the first logical steps is to identify reproductive microbiome features related to fertility and to isolate the fertility-associated microbial species for developing a future bacterial consortium that could be administered before breeding to enhance pregnancy outcomes. Here, we characterized the vaginal and uterine microbiota in beef cattle that became pregnant or remained open via artificial insemination and identified microbiota features associated with fertility. We compared similarities between vaginal and uterine microbiota and between heifers and cows. Using culturing, we provided new insights into the culturable fraction of the vaginal and uterine microbiota and their antimicrobial resistance. Overall, our findings will serve as an important basis for future research aimed at harnessing the vaginal and uterine microbiome for improved cattle fertility.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sequencing and culture-based characterization of the vaginal and uterine microbiota in beef cattle that became pregnant or remained open following artificial insemination

Webb,

Holman,

Schmidt

et al. 2023

Microbiol Spectr

View full text Add to dashboard Cite

show abstract

“…Non-pregnant cows also harbored a greater relative abundance of ASVs identified as Rikenellaceae RC9 gut group (ASV506 and Christensenellaceae R-7 group ASV6112 in their uterus. Although both of these genera are part of the common microbiota present in the cattle gut (Winders et al, 2023), and Rikenellaceae RC9 gut group also colonizes the bovine uterus (Yagisawa et al, 2023), there are no reports regarding their association with reproductive infections or fertility in cattle. Overall, our results, for the first time, show the presence of pregnancy associated microbial taxa among the bovine uterine microbiota.…”

Section: Discussionmentioning

confidence: 99%

Sequencing and Culture-based Characterization of the Vaginal and Uterine Microbiota in Beef Cattle that Became Pregnant or Non-pregnant via Artificial Insemination

Webb,

Holman,

Schmidt

et al. 2023

Preprint

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In this study, we evaluated the vaginal and uterine microbiota between beef cattle that became pregnant via artificial insemination (AI) and those that did not to identify microbial signature associated with pregnancy. We also characterized the culturable fraction of these microbiota using extensive culturing and screened some vaginal and uterine bacterial isolates for their antimicrobial resistance. For this, vaginal and uterine swabs from two cohorts of Angus-crossbred cattle: mature cows (vaginal and uterine; 27 open and 31 pregnant) and heifers (vaginal; 26 open and 33 pregnant) that were collected before AI were processed for microbiota assessment using 16S rRNA gene sequencing and culturing. Twenty-nine vaginal and uterine bacterial isolates were screened for resistance against 41 antibiotics. Sequencing results revealed 11 taxa that were more abundant in the vaginal samples from non-pregnant heifers compared to pregnant heifers. No differentially abundant taxa were detected in the vaginal samples from pregnant versus non-pregnant cows. Pregnant cows had a distinct uterine microbiota community structure (P= 0.008) and interaction network structure compared to non-pregnant cows. Twenty-eight differentially abundant uterine taxa were observed between the two groups. Community structure and diversity were different between the cow vagina and uterus. A total of 733 bacterial isolates were recovered from vaginal (512) and uterine (221) swabs under aerobic (83 different species) and anaerobic (69 species) culturing. Among these isolates were pathogenic species and those mostly susceptible to tested antibiotics. Overall, our results indicate that pregnancy-associated taxonomic signatures are present in the bovine uterine and vaginal microbiota.ImportanceEmerging evidence suggests that microbiome-targeted approaches may provide a novel opportunity to reduce the incidence of reproductive failures in cattle. To develop such microbiome-based strategies, one of the first logical steps is to identify reproductive microbiome features related to fertility, and isolate the pregnancy associated microbial species for developing a future bacterial consortium that could be administered before breeding to enhance pregnancy outcomes. Here, we characterized the vaginal and uterine microbiota in beef cattle that became pregnant or not via AI and identified some microbiota features associated with pregnancy. We compared similarities between vaginal and uterine microbiota, and between heifers and cows. Using extensive culturing, we provided new insights on the culturable fraction of the vaginal and uterine microbiota, and their antimicrobial resistance. Overall, our findings will serve as an important basis for future research aimed at harnessing the vaginal and uterine microbiome for improved cattle fertility.

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Investigation of Aliarcobacter spp. and Campylobacter spp. in uterine contents of cows: Antibacterial susceptibility and phylogenetic analysis of the isolates

Abay,

Müştak

et al. 2024

Microbial Pathogenesis

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Metataxonomic Analysis of the Uterine Microbiota Associated with Low Fertility in Dairy Cows Using Endometrial Tissues Prior to First Artificial Insemination

Cited by 4 publications

References 64 publications

Sequencing and culture-based characterization of the vaginal and uterine microbiota in beef cattle that became pregnant or remained open following artificial insemination

Sequencing and culture-based characterization of the vaginal and uterine microbiota in beef cattle that became pregnant or remained open following artificial insemination

Sequencing and Culture-based Characterization of the Vaginal and Uterine Microbiota in Beef Cattle that Became Pregnant or Non-pregnant via Artificial Insemination

Investigation of Aliarcobacter spp. and Campylobacter spp. in uterine contents of cows: Antibacterial susceptibility and phylogenetic analysis of the isolates

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