Screening of antioxidant activity and volatile compounds composition of Chamerion angustifolium (L.) Holub ecotypes grown in Lithuania

Abstract

Since biological activity of medicinal plants is dependent on cultivation area, climatic conditions, developmental stage, genetic modifications and other factors, it is important to study flora present in different growing sites and geographical zones. This study was focused on screening of antioxidant activity of C. angustifolium harvested in six different locations in Lithuania. The total contents of phenolic compounds, flavonoids and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity were evaluated by spectrophotometric methods. A correlation between radical scavenging activity and total phenolic compounds content was observed (correlation coefficient 0.98). HPLC with online post-column DPPH radical scavenging reaction detection was used for the separation of extracts. Oenothein B, rutin and one unidentified compound were predominant. Volatile compounds were analysed using solid-phase microextraction coupled with gas chromatography-mass spectrometry. Based on the analysis of volatiles, all samples were classified into two chemotypes: (I) with predominant α- and β-caryophyllenes and (II) with predominant anethole.

Biotransformation of vine tea (Ampelopsis grossedentata) by solid-state fermentation using medicinal fungus Poria cocos

“…The DPPH radical scavenging capacity was calculated according to the Eq. (1) as followed (Kaskoniene et al 2015).…”

Cytotoxic attributes of antiviral plants rich in polyphenolic and volatile compounds

“…TFC was determined following the previously optimized procedure [10,22]. The stock solution was prepared and it consisted of 60.0 ml methanol, 3.0 ml acetic acid (33%), 12.0 ml hexamethylenetetramine (5%), 9.0 ml aluminum chloride (10%) and 60.0 ml bidistilled water.…”

“…Determination of total phenolic content (TPC). TPC was determined following the previously optimized Folin-Ciocalteu method [10,22]. 1.0 ml of the sodium carbonate (4%, pH 10) solution was mixed with 0.033 ml of the sample and 0.033 ml of the Folin-Ciocalteu reagent (2 N).…”

“…Spectrophotometric assays are also used. Such methods are capable of determining certain groups of compounds in the complex mixtures and such techniques help in screening the substances of interest [10,11].…”

“…Capillary electrophoresis is a method that is used for separation of soluble charged, or chargeable analytes [9]. Gas chromatography is mainly used for separation of volatile compounds [10]. Liquid chromatography in the reversed phase mode is mainly used for separating substances according to their different pollarity [11].…”