Screening of antibiotic residues in chicken meat in Bangladesh by thin layer chromatography

Sarker, Yousuf Ali; Hasan, Md. Mehedi; Paul, Torun Kumar; Rashid, Sm. Zamiul; Alam, Md. Nurul; Sikder, Mahmudul Hasan

doi:10.5455/javar.2018.e257

Cited by 60 publications

(61 citation statements)

References 23 publications

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“…Particular concern for human health is the inappropriate use of antibiotics in poultry production and the development of antibiotic resistant strains of bacteria (Sarker et al, 2018). Effective control systems are critical in ensuring product safety, and considerable information is available on how to minimize the risks (FAO, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Antibiotics are extensively used in poultry industry either as growth promoters or to control infectious diseases (Sarker et al, 2018). Concern about antibiotic resistance and its transmission to human is important because these resistant bacteria may colonize the human gastrointestinal tract and may contribute to the development of resistance genes to human through Rfactor, conjugative plasmids or chromosomal elements as reviewed by Kabir (2010).…”

Section: Introductionmentioning

confidence: 99%

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Molecular detection and characterization of Escherichia coli, Salmonella spp. and Campylobacter spp. isolated from broiler meat in Jamalpur, Tangail, Netrokona and Kishoreganj districts of Bangladesh

Islam¹,

Kabir²,

Haque³

et al. 2018

Afr. J. Microbiol. Res.

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The study was conducted to isolate, identify and characterize bacterial samples from broiler meat collected from 20 different upazilla markets of Jamalpur, Tangail, Netrokona and Kishoreganj districts of Bangladesh. A total of 20 samples were subjected to bacteriological isolation and identification, and the isolated bacteria were subjected to antimicrobial susceptibility testing using disc diffusion method. Among the samples, 75% (n=15) were contaminated with Campylobacter spp., 70% (n=14) were with Salmonella species and 85% (n=17) were contaminated with Escherichia coli. The Campylobacter spp., Salmonella spp. and E. coli were isolated and identified by culturing on Blood agar, Xylose-Lysine Deoxycholate (XLD) agar, and MacConky and eosin methylene blue (EMB) agar respectively. Isolates of Campylobacter spp., Salmonella spp. and E. coli were biochemically analyzed. Campylobacter specific 16S rRNA genes were amplified from the isolates. Campylobacter spp. and E. coli isolates were positive to 16S rRNA gene based polymerase chain reaction (PCR). Almost all isolates of Campylobacter spp., Salmonella spp. and E. coli showed highest susceptibility to ciprofloxacin, norfloxacin and gentamicin. However, most isolates were resistant to amoxicillin and erythromycin. Some isolates showed susceptibility to tetracycline, streptomycin and azithromycin. The findings of this study revealed that there is presence of multidrug resistant isolates of Campylobacter spp., Salmonella spp. and E. coli in broiler meat. Results of this research project demonstrated the high level of microbial contamination and occurrence of pathogenic bacteria in broiler meat sold in markets of Bangladesh.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular detection and characterization of Escherichia coli, Salmonella spp. and Campylobacter spp. isolated from broiler meat in Jamalpur, Tangail, Netrokona and Kishoreganj districts of Bangladesh

Islam¹,

Kabir²,

Haque³

et al. 2018

Afr. J. Microbiol. Res.

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“…Therefore, TMHMM server was applied for predicting transmembrane helices in mcr-1 genes of Salmonella isolates. TMHMM predicted that, there were five transmemebrane domain in the SAUVM-MCR-1 proteins; TMhelix1 (13-35), TMhelix 2 (50-72), TMhelix 3 (79-101), TMhelix 4 (123-145) and TMhelix 5 (158-180) which were spanned in the inner membrane region ( Figure 5 ). The structure of SAUVM-MCR-1 proteins were modeled using I-TASSER server, where N. meningitis EptA (PDB ID 5FGN) acted as the structural template.…”

Section: Resultsmentioning

confidence: 99%

“…It is very significant by virtue of the fact that, it can be transmitted vertically from parent to offspring; this makes its control a challenge. Although vaccination and good hygiene practices are most effective ways to prevent salmonellosis (11), antibiotics are extensively using either as a growth promoter or prophylaxis and therapeutics in poultry industry of Bangladesh (12, 13). Indeed, the widespread misapplication and nonjudicious use of antimicrobial drugs in poultry settings, culminating the development of antimicrobial resistant pathogens like Salmonella (Gyles, 2008, Cantas et al, 2013; Antunes et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Resistance Profile and mcr-1 Gene Detection in Salmonella Isolates from Poultry in Bangladesh: Molecular and Bioinformatics Characterization

Uddin

Smb

Hasan

et al. 2020

Preprint

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Antimicrobial resistance gene mcr-1 has been disseminated globally since its first discovery in Southern China in late 2015. However, the mcr-1 gene had not been identified previously in Salmonella isolates from poultry in Bangladesh. Here, we aimed to explore antimicrobial resistance gene mcr-1 in Salmonella isolates. Eighty two Salmonella isolates were isolated and characterized from suspected poultry specimens received from different zones of the country. A phenotypic disc diffusion assay with 15 antimicrobial agents was performed following CLSI standard. The disk diffusion assay showed that, all of the isolates presented high resistance to colistin (92.68%), oxytetracycline (86.59%), co-trimoxazole (76.83%), ciprofloxacin (73.17%) and enrofloxacin (65.85%). Further, randomly selected 10 Salmonella isolates were analyzed by polymerase chain reaction (PCR) targeting genus-specific invA and antimicrobial (colistin) resistance mcr-1 genes. Five were confirmed for the presence of the mcr-1 gene belonging to Salmonella spp. Further, sequencing followed by phylogenetic analysis revealed divergent evolutionary relation between the LptA and MCR proteins rendering them resistant to colistin. Three-dimensional homology structures of MCR-1 proteins were constructed and verified using different bioinformatics tools. Moreover, molecular docking interactions suggested that, MCR-1 and LptA share a similar substrate binding cavity which could be validated for the functional analysis. The results represent here is the first molecular and in silico analysis of colistin resistance mcr-1 gene of Salmonella in poultry in Bangladesh, which may emphasize the importance of the study on antibiotic resistance genes requiring for national monitoring and strategic surveillance in the country.

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“…In campylobacterial diarrhoea, most of the patients had diarrhoea with vomiting and fever and the onset was mostly sudden which usually lasted for less than a week (Goossens et al, 1990). In human and veterinary practices, microbial resistance to antibiotic agents is a matter of concern all over the world (Sarker et al, 2018). Resistance of Campylobacter species to antimicrobial agents have been reported worldwide (Isenbarger et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Screening of human diarrhoeal samples in Mymensingh city of Bangladesh for the isolation, identification and antimicrobial resistance profiles of Campylobacter spp.

Karmaker¹,

Kabir²,

Haque³

et al. 2018

Afr. J. Microbiol. Res.

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Campylobacter spp. (Campylobacter jejuni and Campylobacter coli) are one of the major cause of foodborne bacterial diarrhoea in human worldwide. This study was conducted for the isolation, identification and antimicrobial resistance profiling of Campylobacter spp. from diarrhoeal samples of human collected from Surya Kanta (SK) hospital, Mymensingh Medical College, Mymensingh during the period of August 2016 to October 2017. Using cultural and biochemical techniques, a total number of 150 samples were subjected to Campylobacter isolation and identification. The isolated Campylobacter species (C. jejuni and C. coli) were characterized by antimicrobial susceptibility testing. Among 40 positive Campylobacter isolates, 23 (57.50%) were C. jejuni and the rest 17 (42.50%) isolates were C. coli. Furthermore, out of 40 Campylobacter like organisms, 22 Campylobacter isolates were found in male patient and 18 Campylobacter isolates were found in female. 13 (16.04%) C. jejuni and 9 (11.11%) C. coli were found in male and 10 (14.49%) C. jejuni and 8 (11.59%) C. coli were found in female. Considering the different age groups, 5 (33.33%), 12 (27.91%), 19 (29.68%) and 4 (14.28%) Campylobacter isolates were found in 1 to 15, 16 to 30, 31 to 50 and above 50 years respectively during the period of August 2016 to October 2017. Majority of the Campylobacter jejuni were resistant to ampicillin, nalidixic acid, tetracycline and norfloxacin. However, majority of the Campylobacter jejuni were susceptible to gentamycin, chloramphenicol, ciprofloxacin, azithromycin and streptomycin. Furthermore, C. coli were resistant to ampicillin, tetracycline, erythromycin, nalidixic acid, norfloxacin and susceptible to streptomycin, chloramphenicol, gentamycin and ciprofloxacin. Out of 40 Campylobacter isolates 65.21% C. jejuni and 52.94% Campylobacter coli were detected as multidrug resistant. The findings of the study revealed the presence of multidrug resistant Campylobacter species in human diarrhoeal samples in Mymensingh.

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Screening of antibiotic residues in chicken meat in Bangladesh by thin layer chromatography

Cited by 60 publications

References 23 publications

Molecular detection and characterization of Escherichia coli, Salmonella spp. and Campylobacter spp. isolated from broiler meat in Jamalpur, Tangail, Netrokona and Kishoreganj districts of Bangladesh

Molecular detection and characterization of Escherichia coli, Salmonella spp. and Campylobacter spp. isolated from broiler meat in Jamalpur, Tangail, Netrokona and Kishoreganj districts of Bangladesh

Antimicrobial Resistance Profile and mcr-1 Gene Detection in Salmonella Isolates from Poultry in Bangladesh: Molecular and Bioinformatics Characterization

Screening of human diarrhoeal samples in Mymensingh city of Bangladesh for the isolation, identification and antimicrobial resistance profiles of Campylobacter spp.

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